Anaerobic degradation of oleic acid by suspended and granular sludge : identification of palmitic acid as a key intermediate

Detalhes bibliográficos
Autor(a) principal: Pereira, M. A.
Data de Publicação: 2002
Outros Autores: Pires, O. C., Mota, M., Alves, M. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/1055
Resumo: The aim of the present work was to study the maximum potential methane production in batch assays of sludge samples taken along the operation of two EGSB reactors (RI inoculated with granular sludge and RII inoculated with suspended sludge) fed with increasing oleic acid concentrations between 2 and 8 gCOD/l (HRT = 1 day). After removing the residual substrate, the sludge was incubated in batch vials without any added carbon source. A maximum methane production rate of 152 ± 21 mlCH4(STP)/gVS.day was obtained for the suspended sludge taken on day 70, when oleate at a concentration of 2 g COD/l was fed with a co-substrate (50% COD). The maximum plateau achieved in the methane production curve was 1145 ± 307 mlCH4(STP)/gVS, obtained for the suspended sludge taken on day 162, when oleate was fed as the sole carbon source at 6 g COD/l. The methanization rate of the adsorbed substrate was enhanced under stirring conditions and was inhibited by adding oleic acid. Extraction and GC analysis confirmed that the main adsorbed substrate was palmitate, and not oleate. Accumulated palmitate adsorbed onto the sludge and further β-oxidation was inhibited when in the presence of oleic acid. If oleic acid was removed from the medium β-oxidation proceeded with methane production. Suspended sludge was more efficient than granular sludge.
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spelling Anaerobic degradation of oleic acid by suspended and granular sludge : identification of palmitic acid as a key intermediateEGSBGranular sludgeLCFAOleic acidPalmitic acidSuspended sludgeScience & TechnologyThe aim of the present work was to study the maximum potential methane production in batch assays of sludge samples taken along the operation of two EGSB reactors (RI inoculated with granular sludge and RII inoculated with suspended sludge) fed with increasing oleic acid concentrations between 2 and 8 gCOD/l (HRT = 1 day). After removing the residual substrate, the sludge was incubated in batch vials without any added carbon source. A maximum methane production rate of 152 ± 21 mlCH4(STP)/gVS.day was obtained for the suspended sludge taken on day 70, when oleate at a concentration of 2 g COD/l was fed with a co-substrate (50% COD). The maximum plateau achieved in the methane production curve was 1145 ± 307 mlCH4(STP)/gVS, obtained for the suspended sludge taken on day 162, when oleate was fed as the sole carbon source at 6 g COD/l. The methanization rate of the adsorbed substrate was enhanced under stirring conditions and was inhibited by adding oleic acid. Extraction and GC analysis confirmed that the main adsorbed substrate was palmitate, and not oleate. Accumulated palmitate adsorbed onto the sludge and further β-oxidation was inhibited when in the presence of oleic acid. If oleic acid was removed from the medium β-oxidation proceeded with methane production. Suspended sludge was more efficient than granular sludge.IWA PublishingUniversidade do MinhoPereira, M. A.Pires, O. C.Mota, M.Alves, M. M.20022002-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/1055eng“Water Science & Technology”. 45:10 (2002) 139-144.0273-122312188534info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:10:54Zoai:repositorium.sdum.uminho.pt:1822/1055Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:02:35.855673Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Anaerobic degradation of oleic acid by suspended and granular sludge : identification of palmitic acid as a key intermediate
title Anaerobic degradation of oleic acid by suspended and granular sludge : identification of palmitic acid as a key intermediate
spellingShingle Anaerobic degradation of oleic acid by suspended and granular sludge : identification of palmitic acid as a key intermediate
Pereira, M. A.
EGSB
Granular sludge
LCFA
Oleic acid
Palmitic acid
Suspended sludge
Science & Technology
title_short Anaerobic degradation of oleic acid by suspended and granular sludge : identification of palmitic acid as a key intermediate
title_full Anaerobic degradation of oleic acid by suspended and granular sludge : identification of palmitic acid as a key intermediate
title_fullStr Anaerobic degradation of oleic acid by suspended and granular sludge : identification of palmitic acid as a key intermediate
title_full_unstemmed Anaerobic degradation of oleic acid by suspended and granular sludge : identification of palmitic acid as a key intermediate
title_sort Anaerobic degradation of oleic acid by suspended and granular sludge : identification of palmitic acid as a key intermediate
author Pereira, M. A.
author_facet Pereira, M. A.
Pires, O. C.
Mota, M.
Alves, M. M.
author_role author
author2 Pires, O. C.
Mota, M.
Alves, M. M.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Pereira, M. A.
Pires, O. C.
Mota, M.
Alves, M. M.
dc.subject.por.fl_str_mv EGSB
Granular sludge
LCFA
Oleic acid
Palmitic acid
Suspended sludge
Science & Technology
topic EGSB
Granular sludge
LCFA
Oleic acid
Palmitic acid
Suspended sludge
Science & Technology
description The aim of the present work was to study the maximum potential methane production in batch assays of sludge samples taken along the operation of two EGSB reactors (RI inoculated with granular sludge and RII inoculated with suspended sludge) fed with increasing oleic acid concentrations between 2 and 8 gCOD/l (HRT = 1 day). After removing the residual substrate, the sludge was incubated in batch vials without any added carbon source. A maximum methane production rate of 152 ± 21 mlCH4(STP)/gVS.day was obtained for the suspended sludge taken on day 70, when oleate at a concentration of 2 g COD/l was fed with a co-substrate (50% COD). The maximum plateau achieved in the methane production curve was 1145 ± 307 mlCH4(STP)/gVS, obtained for the suspended sludge taken on day 162, when oleate was fed as the sole carbon source at 6 g COD/l. The methanization rate of the adsorbed substrate was enhanced under stirring conditions and was inhibited by adding oleic acid. Extraction and GC analysis confirmed that the main adsorbed substrate was palmitate, and not oleate. Accumulated palmitate adsorbed onto the sludge and further β-oxidation was inhibited when in the presence of oleic acid. If oleic acid was removed from the medium β-oxidation proceeded with methane production. Suspended sludge was more efficient than granular sludge.
publishDate 2002
dc.date.none.fl_str_mv 2002
2002-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/1055
url http://hdl.handle.net/1822/1055
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv “Water Science & Technology”. 45:10 (2002) 139-144.
0273-1223
12188534
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv IWA Publishing
publisher.none.fl_str_mv IWA Publishing
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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