Comparative proteomic analysis of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) tropical and temperate lineages
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/116514 |
Resumo: | The tick vector Rhipicephalus sanguineus is established as a complex of closely related species with high veterinary-medical significance, in which the presence of different genetic, morphological, and biological traits has resulted in the recognition of different lineages within taxa. One of the most striking differences in the "temperate" and "tropical" lineages of R. sanguineus (s.l.) is the vector competence to Ehrlichia canis, suggesting that these ticks tolerate and react differently to pathogen infection. The present study addresses the SG and MG proteome of the R. sanguineus tropical and temperate lineages and compares their proteomic profile during E. canis infection. Batches of nymphs from the two lineages were allowed to feed on naïve and experimentally E. canis infected dogs and after molting, adults were dissected, and salivary glands and midgut tissues separated. Samples were screened for the presence of E. canis before proteomic analyses. The representation of the proteins identified in infected and non-infected tissues of each lineage was compared and gene ontology used for protein classification. Results highlight important differences in those proteomic profiles that added to previous reported genetic, biological, behavioral, and morphological differences, strengthening the hypothesis of the existence of two different species. Comparing infected and non-infected tissues, the results show that, while in midgut tissues the response to E. canis infection is similar in the salivary glands, the two lineages show a different pattern of protein representation. Focusing on the proteins found only in the infected condition, the data suggests that the cement cone produced during tick feeding may be implicated in pathogen infection. This study adds useful information to the debate on the controversial R. sanguineus systematic status, to the discussion related with the different vectorial competence occurring between the two lineages and identifies potential targets for efficient tick and tick-borne disease control. |
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Comparative proteomic analysis of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) tropical and temperate lineagesuncovering differences during Ehrlichia canis infectionTicksProteomicsMialomeSialomeVector competenceEhrlichiaRhipicephalus sanguineusSDG 3 - Good Health and Well-beingSDG 15 - Life on LandThe tick vector Rhipicephalus sanguineus is established as a complex of closely related species with high veterinary-medical significance, in which the presence of different genetic, morphological, and biological traits has resulted in the recognition of different lineages within taxa. One of the most striking differences in the "temperate" and "tropical" lineages of R. sanguineus (s.l.) is the vector competence to Ehrlichia canis, suggesting that these ticks tolerate and react differently to pathogen infection. The present study addresses the SG and MG proteome of the R. sanguineus tropical and temperate lineages and compares their proteomic profile during E. canis infection. Batches of nymphs from the two lineages were allowed to feed on naïve and experimentally E. canis infected dogs and after molting, adults were dissected, and salivary glands and midgut tissues separated. Samples were screened for the presence of E. canis before proteomic analyses. The representation of the proteins identified in infected and non-infected tissues of each lineage was compared and gene ontology used for protein classification. Results highlight important differences in those proteomic profiles that added to previous reported genetic, biological, behavioral, and morphological differences, strengthening the hypothesis of the existence of two different species. Comparing infected and non-infected tissues, the results show that, while in midgut tissues the response to E. canis infection is similar in the salivary glands, the two lineages show a different pattern of protein representation. Focusing on the proteins found only in the infected condition, the data suggests that the cement cone produced during tick feeding may be implicated in pathogen infection. This study adds useful information to the debate on the controversial R. sanguineus systematic status, to the discussion related with the different vectorial competence occurring between the two lineages and identifies potential targets for efficient tick and tick-borne disease control.Vector borne diseases and pathogens (VBD)Global Health and Tropical Medicine (GHTM)Instituto de Higiene e Medicina Tropical (IHMT)RUNSanches, Gustavo SeronVillar, MargaritaCouto, JoanaFerrolho, J.Fernández de Mera, Isabel G.André, Marcos RogérioBarros-Battesti, Darci MoraesMachado, Rosangela ZacariasBechara, Gervásio HenriqueMateos-Hernández, LourdesDe La Fuente, JoséAntunes, S.Domingos, A.2021-04-30T22:44:06Z2021-012021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article20application/pdfhttp://hdl.handle.net/10362/116514eng2235-2988PURE: 27877324https://doi.org/10.3389/fcimb.2020.611113info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:52:19Zoai:run.unl.pt:10362/116514Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:52:19Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Comparative proteomic analysis of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) tropical and temperate lineages uncovering differences during Ehrlichia canis infection |
title |
Comparative proteomic analysis of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) tropical and temperate lineages |
spellingShingle |
Comparative proteomic analysis of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) tropical and temperate lineages Sanches, Gustavo Seron Ticks Proteomics Mialome Sialome Vector competence Ehrlichia Rhipicephalus sanguineus SDG 3 - Good Health and Well-being SDG 15 - Life on Land |
title_short |
Comparative proteomic analysis of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) tropical and temperate lineages |
title_full |
Comparative proteomic analysis of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) tropical and temperate lineages |
title_fullStr |
Comparative proteomic analysis of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) tropical and temperate lineages |
title_full_unstemmed |
Comparative proteomic analysis of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) tropical and temperate lineages |
title_sort |
Comparative proteomic analysis of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) tropical and temperate lineages |
author |
Sanches, Gustavo Seron |
author_facet |
Sanches, Gustavo Seron Villar, Margarita Couto, Joana Ferrolho, J. Fernández de Mera, Isabel G. André, Marcos Rogério Barros-Battesti, Darci Moraes Machado, Rosangela Zacarias Bechara, Gervásio Henrique Mateos-Hernández, Lourdes De La Fuente, José Antunes, S. Domingos, A. |
author_role |
author |
author2 |
Villar, Margarita Couto, Joana Ferrolho, J. Fernández de Mera, Isabel G. André, Marcos Rogério Barros-Battesti, Darci Moraes Machado, Rosangela Zacarias Bechara, Gervásio Henrique Mateos-Hernández, Lourdes De La Fuente, José Antunes, S. Domingos, A. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Vector borne diseases and pathogens (VBD) Global Health and Tropical Medicine (GHTM) Instituto de Higiene e Medicina Tropical (IHMT) RUN |
dc.contributor.author.fl_str_mv |
Sanches, Gustavo Seron Villar, Margarita Couto, Joana Ferrolho, J. Fernández de Mera, Isabel G. André, Marcos Rogério Barros-Battesti, Darci Moraes Machado, Rosangela Zacarias Bechara, Gervásio Henrique Mateos-Hernández, Lourdes De La Fuente, José Antunes, S. Domingos, A. |
dc.subject.por.fl_str_mv |
Ticks Proteomics Mialome Sialome Vector competence Ehrlichia Rhipicephalus sanguineus SDG 3 - Good Health and Well-being SDG 15 - Life on Land |
topic |
Ticks Proteomics Mialome Sialome Vector competence Ehrlichia Rhipicephalus sanguineus SDG 3 - Good Health and Well-being SDG 15 - Life on Land |
description |
The tick vector Rhipicephalus sanguineus is established as a complex of closely related species with high veterinary-medical significance, in which the presence of different genetic, morphological, and biological traits has resulted in the recognition of different lineages within taxa. One of the most striking differences in the "temperate" and "tropical" lineages of R. sanguineus (s.l.) is the vector competence to Ehrlichia canis, suggesting that these ticks tolerate and react differently to pathogen infection. The present study addresses the SG and MG proteome of the R. sanguineus tropical and temperate lineages and compares their proteomic profile during E. canis infection. Batches of nymphs from the two lineages were allowed to feed on naïve and experimentally E. canis infected dogs and after molting, adults were dissected, and salivary glands and midgut tissues separated. Samples were screened for the presence of E. canis before proteomic analyses. The representation of the proteins identified in infected and non-infected tissues of each lineage was compared and gene ontology used for protein classification. Results highlight important differences in those proteomic profiles that added to previous reported genetic, biological, behavioral, and morphological differences, strengthening the hypothesis of the existence of two different species. Comparing infected and non-infected tissues, the results show that, while in midgut tissues the response to E. canis infection is similar in the salivary glands, the two lineages show a different pattern of protein representation. Focusing on the proteins found only in the infected condition, the data suggests that the cement cone produced during tick feeding may be implicated in pathogen infection. This study adds useful information to the debate on the controversial R. sanguineus systematic status, to the discussion related with the different vectorial competence occurring between the two lineages and identifies potential targets for efficient tick and tick-borne disease control. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-04-30T22:44:06Z 2021-01 2021-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/116514 |
url |
http://hdl.handle.net/10362/116514 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2235-2988 PURE: 27877324 https://doi.org/10.3389/fcimb.2020.611113 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
20 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817545793561690112 |