Raineya orbicola gen. nov., sp. nov. a slightly thermophilic bacterium of the phylum bacteroidetes and the description of raineyaceae fam. nov
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/10216/120374 |
Resumo: | An isolate, designated SPSPC-11T, with an optimum growth temperature of about 50°C and an optimum pH for growth between 7.5 and 8.0, was recovered from a hot spring in central Portugal. Based on phylogenetic analysis of its 16S rRNA sequence, the new organism is most closely related to the species of the genus Thermonema but with a pairwise sequence similarity of <85 %. The isolate was orange-pigmented, formed non-motile long filaments and rod-shaped cells that stain Gram-negative. The organism was strictly aerobic, oxidase-positive and catalase-positive. The major fatty acids were iso- C15:0, iso-C15: 0 2-OH and iso-C17: 0 3-OH. The major polar lipids were one aminophospholipid, two aminolipids and three unidentified lipids. Menaquinone 7 was the major respiratory quinone. The DNA G+C content of strain SPSPC-11T was 37.6 mol% (draft genome sequence). The high quality draft genome sequence corroborated many of the phenotypic characteristics of strain SPSPC-11T. Based on genotypic, phylogenetic, physiological and biochemical characterization we describe a new species of a novel genus represented by strain SPSPC-11T (=CECT 9012T=LMG 29233T) for which we propose the name Raineya orbicola gen. nov., sp. nov. We also describe the family Raineyaceae to accommodate this new genus and species. © 2018 IUMS. |
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Raineya orbicola gen. nov., sp. nov. a slightly thermophilic bacterium of the phylum bacteroidetes and the description of raineyaceae fam. novaminophospholipidcatalasefatty acidmenaquinoneoxidoreductasequinone derivativeRNA 16Sbacterial DNAfarnoquinonephospholipidvitamin MK 7Articlebacterial growthbacterium isolateBacteroidetesDNA base compositiongene sequencenonhumanpHphylogenyPortugalpriority journalRaineya orbicolaRNA sequencescanning electron microscopytemperaturethermal springthermophilic bacteriumtransmission electron microscopyanalogs and derivativesbacterium identificationBacteroideteschemistryclassificationDNA sequencegeneticsisolation and purificationmicrobiologyphylogenypigmentationBacterial Typing TechniquesBacteroidetesBase CompositionDNA, BacterialFatty AcidsHot SpringsPhospholipidsPhylogenyPigmentationPortugalRNA, Ribosomal, 16SSequence Analysis, DNAVitamin K 2An isolate, designated SPSPC-11T, with an optimum growth temperature of about 50°C and an optimum pH for growth between 7.5 and 8.0, was recovered from a hot spring in central Portugal. Based on phylogenetic analysis of its 16S rRNA sequence, the new organism is most closely related to the species of the genus Thermonema but with a pairwise sequence similarity of <85 %. The isolate was orange-pigmented, formed non-motile long filaments and rod-shaped cells that stain Gram-negative. The organism was strictly aerobic, oxidase-positive and catalase-positive. The major fatty acids were iso- C15:0, iso-C15: 0 2-OH and iso-C17: 0 3-OH. The major polar lipids were one aminophospholipid, two aminolipids and three unidentified lipids. Menaquinone 7 was the major respiratory quinone. The DNA G+C content of strain SPSPC-11T was 37.6 mol% (draft genome sequence). The high quality draft genome sequence corroborated many of the phenotypic characteristics of strain SPSPC-11T. Based on genotypic, phylogenetic, physiological and biochemical characterization we describe a new species of a novel genus represented by strain SPSPC-11T (=CECT 9012T=LMG 29233T) for which we propose the name Raineya orbicola gen. nov., sp. nov. We also describe the family Raineyaceae to accommodate this new genus and species. © 2018 IUMS.Microbiology Society20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/120374eng14665034, 1466502610.1099/ijsem.0.002556Albuquerque L.Polónia A.R.M.Barroso C.Froufe H.J.C.Lage O.Lobo-Da-Cunha A.Egas C.Da Costa M.S.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T13:59:26Zoai:repositorio-aberto.up.pt:10216/120374Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:51:45.568680Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Raineya orbicola gen. nov., sp. nov. a slightly thermophilic bacterium of the phylum bacteroidetes and the description of raineyaceae fam. nov |
title |
Raineya orbicola gen. nov., sp. nov. a slightly thermophilic bacterium of the phylum bacteroidetes and the description of raineyaceae fam. nov |
spellingShingle |
Raineya orbicola gen. nov., sp. nov. a slightly thermophilic bacterium of the phylum bacteroidetes and the description of raineyaceae fam. nov Albuquerque L. aminophospholipid catalase fatty acid menaquinone oxidoreductase quinone derivative RNA 16S bacterial DNA farnoquinone phospholipid vitamin MK 7 Article bacterial growth bacterium isolate Bacteroidetes DNA base composition gene sequence nonhuman pH phylogeny Portugal priority journal Raineya orbicola RNA sequence scanning electron microscopy temperature thermal spring thermophilic bacterium transmission electron microscopy analogs and derivatives bacterium identification Bacteroidetes chemistry classification DNA sequence genetics isolation and purification microbiology phylogeny pigmentation Bacterial Typing Techniques Bacteroidetes Base Composition DNA, Bacterial Fatty Acids Hot Springs Phospholipids Phylogeny Pigmentation Portugal RNA, Ribosomal, 16S Sequence Analysis, DNA Vitamin K 2 |
title_short |
Raineya orbicola gen. nov., sp. nov. a slightly thermophilic bacterium of the phylum bacteroidetes and the description of raineyaceae fam. nov |
title_full |
Raineya orbicola gen. nov., sp. nov. a slightly thermophilic bacterium of the phylum bacteroidetes and the description of raineyaceae fam. nov |
title_fullStr |
Raineya orbicola gen. nov., sp. nov. a slightly thermophilic bacterium of the phylum bacteroidetes and the description of raineyaceae fam. nov |
title_full_unstemmed |
Raineya orbicola gen. nov., sp. nov. a slightly thermophilic bacterium of the phylum bacteroidetes and the description of raineyaceae fam. nov |
title_sort |
Raineya orbicola gen. nov., sp. nov. a slightly thermophilic bacterium of the phylum bacteroidetes and the description of raineyaceae fam. nov |
author |
Albuquerque L. |
author_facet |
Albuquerque L. Polónia A.R.M. Barroso C. Froufe H.J.C. Lage O. Lobo-Da-Cunha A. Egas C. Da Costa M.S. |
author_role |
author |
author2 |
Polónia A.R.M. Barroso C. Froufe H.J.C. Lage O. Lobo-Da-Cunha A. Egas C. Da Costa M.S. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Albuquerque L. Polónia A.R.M. Barroso C. Froufe H.J.C. Lage O. Lobo-Da-Cunha A. Egas C. Da Costa M.S. |
dc.subject.por.fl_str_mv |
aminophospholipid catalase fatty acid menaquinone oxidoreductase quinone derivative RNA 16S bacterial DNA farnoquinone phospholipid vitamin MK 7 Article bacterial growth bacterium isolate Bacteroidetes DNA base composition gene sequence nonhuman pH phylogeny Portugal priority journal Raineya orbicola RNA sequence scanning electron microscopy temperature thermal spring thermophilic bacterium transmission electron microscopy analogs and derivatives bacterium identification Bacteroidetes chemistry classification DNA sequence genetics isolation and purification microbiology phylogeny pigmentation Bacterial Typing Techniques Bacteroidetes Base Composition DNA, Bacterial Fatty Acids Hot Springs Phospholipids Phylogeny Pigmentation Portugal RNA, Ribosomal, 16S Sequence Analysis, DNA Vitamin K 2 |
topic |
aminophospholipid catalase fatty acid menaquinone oxidoreductase quinone derivative RNA 16S bacterial DNA farnoquinone phospholipid vitamin MK 7 Article bacterial growth bacterium isolate Bacteroidetes DNA base composition gene sequence nonhuman pH phylogeny Portugal priority journal Raineya orbicola RNA sequence scanning electron microscopy temperature thermal spring thermophilic bacterium transmission electron microscopy analogs and derivatives bacterium identification Bacteroidetes chemistry classification DNA sequence genetics isolation and purification microbiology phylogeny pigmentation Bacterial Typing Techniques Bacteroidetes Base Composition DNA, Bacterial Fatty Acids Hot Springs Phospholipids Phylogeny Pigmentation Portugal RNA, Ribosomal, 16S Sequence Analysis, DNA Vitamin K 2 |
description |
An isolate, designated SPSPC-11T, with an optimum growth temperature of about 50°C and an optimum pH for growth between 7.5 and 8.0, was recovered from a hot spring in central Portugal. Based on phylogenetic analysis of its 16S rRNA sequence, the new organism is most closely related to the species of the genus Thermonema but with a pairwise sequence similarity of <85 %. The isolate was orange-pigmented, formed non-motile long filaments and rod-shaped cells that stain Gram-negative. The organism was strictly aerobic, oxidase-positive and catalase-positive. The major fatty acids were iso- C15:0, iso-C15: 0 2-OH and iso-C17: 0 3-OH. The major polar lipids were one aminophospholipid, two aminolipids and three unidentified lipids. Menaquinone 7 was the major respiratory quinone. The DNA G+C content of strain SPSPC-11T was 37.6 mol% (draft genome sequence). The high quality draft genome sequence corroborated many of the phenotypic characteristics of strain SPSPC-11T. Based on genotypic, phylogenetic, physiological and biochemical characterization we describe a new species of a novel genus represented by strain SPSPC-11T (=CECT 9012T=LMG 29233T) for which we propose the name Raineya orbicola gen. nov., sp. nov. We also describe the family Raineyaceae to accommodate this new genus and species. © 2018 IUMS. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 2018-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/10216/120374 |
url |
https://hdl.handle.net/10216/120374 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
14665034, 14665026 10.1099/ijsem.0.002556 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Microbiology Society |
publisher.none.fl_str_mv |
Microbiology Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799135837848338432 |