Isolation and characterization of polysaccharides from the ascidian Styela clava
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/78228 |
Resumo: | <i>Styela clava</i> is an edible sea squirt farmed in Korea that has gradually invaded other seas, negatively impacting the ecology and economy of coastal areas. Extracts from <i>S. clava</i> have shown wide bioactivities, and ascidians have the unique capability among animals of biosynthesizing cellulose. Thus, <i>S. clava</i> is a relevant candidate for valorization. Herein, we aimed at surveying and characterizing polysaccharides in both tunic and flesh of this ascidian. To this end, we enzymatically hydrolyzed both tissues, recovering crystalline cellulose from the tunic with high aspect ratios, based on results from microscopy, X-ray diffraction, and infrared spectroscopy analyses. Alkaline hydroalcoholic precipitation was applied to isolate the polysaccharide fraction that was characterized by gel permeation chromatography (with light scattering detection) and NMR. These techniques allowed the identification of glycogen in the flesh with an estimated Mw of 7 MDa. Tunic polysaccharides consisted of two fractions of different Mw. Application of Diffusion-Ordered NMR allowed spectroscopically separating the low-molecular-weight fraction to analyze the major component of an estimated Mw of 40–66 kDa. We identified six different sugar residues, although its complexity prevented the determination of the complete structure and connectivities of the residues. The two more abundant residues were N-acetylated and possibly components of the glycosaminoglycan-like (GAG-like) family, showing the remaining similarities to sulfated galactans. Therefore, <i>Styela clava</i> appears as a source of nanocrystalline cellulose and GAG-like polysaccharides. |
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Isolation and characterization of polysaccharides from the ascidian Styela clavaStyela clavaAscidianCellulosePolysaccharidesTunicateGlycosaminoglycansValorizationScience & Technology<i>Styela clava</i> is an edible sea squirt farmed in Korea that has gradually invaded other seas, negatively impacting the ecology and economy of coastal areas. Extracts from <i>S. clava</i> have shown wide bioactivities, and ascidians have the unique capability among animals of biosynthesizing cellulose. Thus, <i>S. clava</i> is a relevant candidate for valorization. Herein, we aimed at surveying and characterizing polysaccharides in both tunic and flesh of this ascidian. To this end, we enzymatically hydrolyzed both tissues, recovering crystalline cellulose from the tunic with high aspect ratios, based on results from microscopy, X-ray diffraction, and infrared spectroscopy analyses. Alkaline hydroalcoholic precipitation was applied to isolate the polysaccharide fraction that was characterized by gel permeation chromatography (with light scattering detection) and NMR. These techniques allowed the identification of glycogen in the flesh with an estimated Mw of 7 MDa. Tunic polysaccharides consisted of two fractions of different Mw. Application of Diffusion-Ordered NMR allowed spectroscopically separating the low-molecular-weight fraction to analyze the major component of an estimated Mw of 40–66 kDa. We identified six different sugar residues, although its complexity prevented the determination of the complete structure and connectivities of the residues. The two more abundant residues were N-acetylated and possibly components of the glycosaminoglycan-like (GAG-like) family, showing the remaining similarities to sulfated galactans. Therefore, <i>Styela clava</i> appears as a source of nanocrystalline cellulose and GAG-like polysaccharides.This research was funded by Xunta de Galicia (Grupos de Potencial Crecimiento, IN607B 2021/11) and Fundação para a Ciência e a Tecnologia (Grant No. PTDC/QUI-POL/28117/2017 and CEECIND/00814/2017).Multidisciplinary Digital Publishing Institute (MDPI)Universidade do MinhoValcarcel, JesusVázquez, José AntonioVarela, Uxía R.Reis, R. L.Novoa-Carballal, Ramon20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/78228engValcarcel, J.; Vázquez, J.A.; Varela, U.R.; Reis, R.L.; Novoa-Carballal, R. Isolation and Characterization of Polysaccharides from the Ascidian Styela clava. Polymers 2022, 14, 16. https://doi.org/10.3390/polym140100162073-436010.3390/polym1401001616https://www.mdpi.com/2073-4360/14/1/16info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-09-16T01:16:31Zoai:repositorium.sdum.uminho.pt:1822/78228Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:51:55.411927Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Isolation and characterization of polysaccharides from the ascidian Styela clava |
title |
Isolation and characterization of polysaccharides from the ascidian Styela clava |
spellingShingle |
Isolation and characterization of polysaccharides from the ascidian Styela clava Valcarcel, Jesus Styela clava Ascidian Cellulose Polysaccharides Tunicate Glycosaminoglycans Valorization Science & Technology |
title_short |
Isolation and characterization of polysaccharides from the ascidian Styela clava |
title_full |
Isolation and characterization of polysaccharides from the ascidian Styela clava |
title_fullStr |
Isolation and characterization of polysaccharides from the ascidian Styela clava |
title_full_unstemmed |
Isolation and characterization of polysaccharides from the ascidian Styela clava |
title_sort |
Isolation and characterization of polysaccharides from the ascidian Styela clava |
author |
Valcarcel, Jesus |
author_facet |
Valcarcel, Jesus Vázquez, José Antonio Varela, Uxía R. Reis, R. L. Novoa-Carballal, Ramon |
author_role |
author |
author2 |
Vázquez, José Antonio Varela, Uxía R. Reis, R. L. Novoa-Carballal, Ramon |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Valcarcel, Jesus Vázquez, José Antonio Varela, Uxía R. Reis, R. L. Novoa-Carballal, Ramon |
dc.subject.por.fl_str_mv |
Styela clava Ascidian Cellulose Polysaccharides Tunicate Glycosaminoglycans Valorization Science & Technology |
topic |
Styela clava Ascidian Cellulose Polysaccharides Tunicate Glycosaminoglycans Valorization Science & Technology |
description |
<i>Styela clava</i> is an edible sea squirt farmed in Korea that has gradually invaded other seas, negatively impacting the ecology and economy of coastal areas. Extracts from <i>S. clava</i> have shown wide bioactivities, and ascidians have the unique capability among animals of biosynthesizing cellulose. Thus, <i>S. clava</i> is a relevant candidate for valorization. Herein, we aimed at surveying and characterizing polysaccharides in both tunic and flesh of this ascidian. To this end, we enzymatically hydrolyzed both tissues, recovering crystalline cellulose from the tunic with high aspect ratios, based on results from microscopy, X-ray diffraction, and infrared spectroscopy analyses. Alkaline hydroalcoholic precipitation was applied to isolate the polysaccharide fraction that was characterized by gel permeation chromatography (with light scattering detection) and NMR. These techniques allowed the identification of glycogen in the flesh with an estimated Mw of 7 MDa. Tunic polysaccharides consisted of two fractions of different Mw. Application of Diffusion-Ordered NMR allowed spectroscopically separating the low-molecular-weight fraction to analyze the major component of an estimated Mw of 40–66 kDa. We identified six different sugar residues, although its complexity prevented the determination of the complete structure and connectivities of the residues. The two more abundant residues were N-acetylated and possibly components of the glycosaminoglycan-like (GAG-like) family, showing the remaining similarities to sulfated galactans. Therefore, <i>Styela clava</i> appears as a source of nanocrystalline cellulose and GAG-like polysaccharides. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022 2022-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/78228 |
url |
https://hdl.handle.net/1822/78228 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Valcarcel, J.; Vázquez, J.A.; Varela, U.R.; Reis, R.L.; Novoa-Carballal, R. Isolation and Characterization of Polysaccharides from the Ascidian Styela clava. Polymers 2022, 14, 16. https://doi.org/10.3390/polym14010016 2073-4360 10.3390/polym14010016 16 https://www.mdpi.com/2073-4360/14/1/16 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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