Role of inter-species recombination of the ftsI gene in the dissemination of altered penicillin-binding-protein-3-mediated resistance in Haemophilus influenzae and Haemophilus haemolyticus
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/2164 |
Resumo: | Objectives: To screen the ftsI gene sequences obtained from clinical isolates of non-typeable H. influenzae (NTHi), and Haemophilus haemolyticus for the presence of mosaic ftsI gene structures, and to evaluate the role homologous recombination of the ftsI gene has on the formation and distribution of resistant ftsI genes in NTHi. Methods: The ftsI genes of 100 Haemophilus isolates comprising genetically defined β-lactamase-negative ampicillin-susceptible (gBLNAS) and β-lactamase-negative ampicillin-resistant (gBLNAR) isolates of NTHi (n=50) and H. haemolyticus (n=50) were analyzed in this study. The full-length ftsI gene sequences of all study isolates were screened for mosaic ftsI gene structures using H. influenzae Rd and H. haemolyticus ATCC 33390 as reference parental sequences, and were subjected to recombination analysis using bioinformatics software Mega5 and Simplot. Results: Of the 100 clinical isolates analyzed 34% (34/100) harbored mosaic ftsI gene structures containing distinct ftsI gene fragments similar to both reference parental sequences. Inter-species recombination events were predominantly encountered in the ftsI gene of gBLNAR isolates of NTHi and H. haemolyticus, and were always associated with the formation of a mosaic fragment at the 3’ end of the ftsI gene. There was no evidence supporting the horizontal gene transfer (HGT) of the entire ftsI gene among the clinical isolates in vivo. Conclusion: We provide evidence for the HGT and inter-species recombination of the ftsI gene among isolates of NTHi and H. haemolyticus in a clinical setting, highlighting the importance recombination of the ftsI gene has on the emergence of altered PBP3s and BLNAR mediated resistance. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Role of inter-species recombination of the ftsI gene in the dissemination of altered penicillin-binding-protein-3-mediated resistance in Haemophilus influenzae and Haemophilus haemolyticusBLNARβ-lactam ResistanceMosaic GenesHorizontal Gene TransferInfecções RespiratóriasObjectives: To screen the ftsI gene sequences obtained from clinical isolates of non-typeable H. influenzae (NTHi), and Haemophilus haemolyticus for the presence of mosaic ftsI gene structures, and to evaluate the role homologous recombination of the ftsI gene has on the formation and distribution of resistant ftsI genes in NTHi. Methods: The ftsI genes of 100 Haemophilus isolates comprising genetically defined β-lactamase-negative ampicillin-susceptible (gBLNAS) and β-lactamase-negative ampicillin-resistant (gBLNAR) isolates of NTHi (n=50) and H. haemolyticus (n=50) were analyzed in this study. The full-length ftsI gene sequences of all study isolates were screened for mosaic ftsI gene structures using H. influenzae Rd and H. haemolyticus ATCC 33390 as reference parental sequences, and were subjected to recombination analysis using bioinformatics software Mega5 and Simplot. Results: Of the 100 clinical isolates analyzed 34% (34/100) harbored mosaic ftsI gene structures containing distinct ftsI gene fragments similar to both reference parental sequences. Inter-species recombination events were predominantly encountered in the ftsI gene of gBLNAR isolates of NTHi and H. haemolyticus, and were always associated with the formation of a mosaic fragment at the 3’ end of the ftsI gene. There was no evidence supporting the horizontal gene transfer (HGT) of the entire ftsI gene among the clinical isolates in vivo. Conclusion: We provide evidence for the HGT and inter-species recombination of the ftsI gene among isolates of NTHi and H. haemolyticus in a clinical setting, highlighting the importance recombination of the ftsI gene has on the emergence of altered PBP3s and BLNAR mediated resistance.Oxford University Press/ British Society for Antimicrobial ChemotherapyRepositório Científico do Instituto Nacional de SaúdeWitherden, E.A.Bajanca-Lavado, Maria PaulaTristram, S.G.Nunes, A.2014-03-18T12:39:23Z2014-02-202014-02-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/2164engJ Antimicrob Chemother. 2014 Jun;69(6):1501-9. doi: 10.1093/jac/dku022. Epub 2014 Feb 20.0305-7453doi:10.1093/jac/dku022info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:39:07Zoai:repositorio.insa.pt:10400.18/2164Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:37:12.348814Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Role of inter-species recombination of the ftsI gene in the dissemination of altered penicillin-binding-protein-3-mediated resistance in Haemophilus influenzae and Haemophilus haemolyticus |
title |
Role of inter-species recombination of the ftsI gene in the dissemination of altered penicillin-binding-protein-3-mediated resistance in Haemophilus influenzae and Haemophilus haemolyticus |
spellingShingle |
Role of inter-species recombination of the ftsI gene in the dissemination of altered penicillin-binding-protein-3-mediated resistance in Haemophilus influenzae and Haemophilus haemolyticus Witherden, E.A. BLNAR β-lactam Resistance Mosaic Genes Horizontal Gene Transfer Infecções Respiratórias |
title_short |
Role of inter-species recombination of the ftsI gene in the dissemination of altered penicillin-binding-protein-3-mediated resistance in Haemophilus influenzae and Haemophilus haemolyticus |
title_full |
Role of inter-species recombination of the ftsI gene in the dissemination of altered penicillin-binding-protein-3-mediated resistance in Haemophilus influenzae and Haemophilus haemolyticus |
title_fullStr |
Role of inter-species recombination of the ftsI gene in the dissemination of altered penicillin-binding-protein-3-mediated resistance in Haemophilus influenzae and Haemophilus haemolyticus |
title_full_unstemmed |
Role of inter-species recombination of the ftsI gene in the dissemination of altered penicillin-binding-protein-3-mediated resistance in Haemophilus influenzae and Haemophilus haemolyticus |
title_sort |
Role of inter-species recombination of the ftsI gene in the dissemination of altered penicillin-binding-protein-3-mediated resistance in Haemophilus influenzae and Haemophilus haemolyticus |
author |
Witherden, E.A. |
author_facet |
Witherden, E.A. Bajanca-Lavado, Maria Paula Tristram, S.G. Nunes, A. |
author_role |
author |
author2 |
Bajanca-Lavado, Maria Paula Tristram, S.G. Nunes, A. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Witherden, E.A. Bajanca-Lavado, Maria Paula Tristram, S.G. Nunes, A. |
dc.subject.por.fl_str_mv |
BLNAR β-lactam Resistance Mosaic Genes Horizontal Gene Transfer Infecções Respiratórias |
topic |
BLNAR β-lactam Resistance Mosaic Genes Horizontal Gene Transfer Infecções Respiratórias |
description |
Objectives: To screen the ftsI gene sequences obtained from clinical isolates of non-typeable H. influenzae (NTHi), and Haemophilus haemolyticus for the presence of mosaic ftsI gene structures, and to evaluate the role homologous recombination of the ftsI gene has on the formation and distribution of resistant ftsI genes in NTHi. Methods: The ftsI genes of 100 Haemophilus isolates comprising genetically defined β-lactamase-negative ampicillin-susceptible (gBLNAS) and β-lactamase-negative ampicillin-resistant (gBLNAR) isolates of NTHi (n=50) and H. haemolyticus (n=50) were analyzed in this study. The full-length ftsI gene sequences of all study isolates were screened for mosaic ftsI gene structures using H. influenzae Rd and H. haemolyticus ATCC 33390 as reference parental sequences, and were subjected to recombination analysis using bioinformatics software Mega5 and Simplot. Results: Of the 100 clinical isolates analyzed 34% (34/100) harbored mosaic ftsI gene structures containing distinct ftsI gene fragments similar to both reference parental sequences. Inter-species recombination events were predominantly encountered in the ftsI gene of gBLNAR isolates of NTHi and H. haemolyticus, and were always associated with the formation of a mosaic fragment at the 3’ end of the ftsI gene. There was no evidence supporting the horizontal gene transfer (HGT) of the entire ftsI gene among the clinical isolates in vivo. Conclusion: We provide evidence for the HGT and inter-species recombination of the ftsI gene among isolates of NTHi and H. haemolyticus in a clinical setting, highlighting the importance recombination of the ftsI gene has on the emergence of altered PBP3s and BLNAR mediated resistance. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-03-18T12:39:23Z 2014-02-20 2014-02-20T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/2164 |
url |
http://hdl.handle.net/10400.18/2164 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
J Antimicrob Chemother. 2014 Jun;69(6):1501-9. doi: 10.1093/jac/dku022. Epub 2014 Feb 20. 0305-7453 doi:10.1093/jac/dku022 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Oxford University Press/ British Society for Antimicrobial Chemotherapy |
publisher.none.fl_str_mv |
Oxford University Press/ British Society for Antimicrobial Chemotherapy |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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