L-threonine supplementation during colitis onset delays disease recovery
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.3389/fphys.2018.01247 |
Resumo: | Dietary nutrients have emerged as potential therapeutic adjuncts for inflammatory bowel disease (IBD) given their impact on intestinal homeostasis through the modulation of immune response, gut microbiota composition and epithelial barrier stability. Several nutrients have already been associated with a protective phenotype. Yet, there is a lack of knowledge toward the most promising ones as well as the most adequate phase of action. To unveil the most prominent therapy candidates we characterized the colon metabolic profile during colitis development. We have observed a twofold decrease in threonine levels in mice subjected to DSS-induced colitis. We then assessed the effect of threonine supplementation in the beginning of the inflammatory process (DSS + Thr) or when inflammation is already established (DSS + Thr D8). Colitis progression was similar between the treated groups and control colitic mice, yet threonine had a surprisingly detrimental effect when administered in the beginning of the disease, with mice displaying a delayed recovery when compared to control mice and mice supplemented with threonine after day 8. Although no major changes were found in their metabolic profile, DSS + Thr mice displayed altered expression in mucin-encoding genes, as well as in goblet cell counts, unveiling an impaired ability to produce mucus. Moreover, IL-22 secretion was decreased in DSS + Thr mice when compared to DSS + Thr D8 mice. Overall, these results suggest that supplementation with threonine during colitis induction impact goblet cell number and delays the recovery period. This reinforces the importance of a deeper understanding regarding threonine supplementation in IBD. |
id |
RCAP_5ed391819732a68c4b9eeefb6dc94a1f |
---|---|
oai_identifier_str |
oai:run.unl.pt:10362/67802 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
L-threonine supplementation during colitis onset delays disease recoveryDSS-induced colitisGoblet cellsIBDIL-22MetabolomicsMucinThreoninePhysiologyPhysiology (medical)Dietary nutrients have emerged as potential therapeutic adjuncts for inflammatory bowel disease (IBD) given their impact on intestinal homeostasis through the modulation of immune response, gut microbiota composition and epithelial barrier stability. Several nutrients have already been associated with a protective phenotype. Yet, there is a lack of knowledge toward the most promising ones as well as the most adequate phase of action. To unveil the most prominent therapy candidates we characterized the colon metabolic profile during colitis development. We have observed a twofold decrease in threonine levels in mice subjected to DSS-induced colitis. We then assessed the effect of threonine supplementation in the beginning of the inflammatory process (DSS + Thr) or when inflammation is already established (DSS + Thr D8). Colitis progression was similar between the treated groups and control colitic mice, yet threonine had a surprisingly detrimental effect when administered in the beginning of the disease, with mice displaying a delayed recovery when compared to control mice and mice supplemented with threonine after day 8. Although no major changes were found in their metabolic profile, DSS + Thr mice displayed altered expression in mucin-encoding genes, as well as in goblet cell counts, unveiling an impaired ability to produce mucus. Moreover, IL-22 secretion was decreased in DSS + Thr mice when compared to DSS + Thr D8 mice. Overall, these results suggest that supplementation with threonine during colitis induction impact goblet cell number and delays the recovery period. This reinforces the importance of a deeper understanding regarding threonine supplementation in IBD.Molecular, Structural and Cellular Microbiology (MOSTMICRO)Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNGaifem, JoanaGonçalves, Luís G.Dinis-Oliveira, Ricardo J.Cunha, CristinaCarvalho, AgostinhoTorrado, EgídioRodrigues, FernandoSaraiva, MargaridaCastro, António G.Silvestre, Ricardo2019-04-26T22:14:35Z2018-09-052018-09-05T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.3389/fphys.2018.01247eng1664-042XPURE: 12428541http://www.scopus.com/inward/record.url?scp=85053078274&partnerID=8YFLogxKhttps://doi.org/10.3389/fphys.2018.01247info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:32:02Zoai:run.unl.pt:10362/67802Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:34:40.115618Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
L-threonine supplementation during colitis onset delays disease recovery |
title |
L-threonine supplementation during colitis onset delays disease recovery |
spellingShingle |
L-threonine supplementation during colitis onset delays disease recovery Gaifem, Joana DSS-induced colitis Goblet cells IBD IL-22 Metabolomics Mucin Threonine Physiology Physiology (medical) |
title_short |
L-threonine supplementation during colitis onset delays disease recovery |
title_full |
L-threonine supplementation during colitis onset delays disease recovery |
title_fullStr |
L-threonine supplementation during colitis onset delays disease recovery |
title_full_unstemmed |
L-threonine supplementation during colitis onset delays disease recovery |
title_sort |
L-threonine supplementation during colitis onset delays disease recovery |
author |
Gaifem, Joana |
author_facet |
Gaifem, Joana Gonçalves, Luís G. Dinis-Oliveira, Ricardo J. Cunha, Cristina Carvalho, Agostinho Torrado, Egídio Rodrigues, Fernando Saraiva, Margarida Castro, António G. Silvestre, Ricardo |
author_role |
author |
author2 |
Gonçalves, Luís G. Dinis-Oliveira, Ricardo J. Cunha, Cristina Carvalho, Agostinho Torrado, Egídio Rodrigues, Fernando Saraiva, Margarida Castro, António G. Silvestre, Ricardo |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Molecular, Structural and Cellular Microbiology (MOSTMICRO) Instituto de Tecnologia Química e Biológica António Xavier (ITQB) RUN |
dc.contributor.author.fl_str_mv |
Gaifem, Joana Gonçalves, Luís G. Dinis-Oliveira, Ricardo J. Cunha, Cristina Carvalho, Agostinho Torrado, Egídio Rodrigues, Fernando Saraiva, Margarida Castro, António G. Silvestre, Ricardo |
dc.subject.por.fl_str_mv |
DSS-induced colitis Goblet cells IBD IL-22 Metabolomics Mucin Threonine Physiology Physiology (medical) |
topic |
DSS-induced colitis Goblet cells IBD IL-22 Metabolomics Mucin Threonine Physiology Physiology (medical) |
description |
Dietary nutrients have emerged as potential therapeutic adjuncts for inflammatory bowel disease (IBD) given their impact on intestinal homeostasis through the modulation of immune response, gut microbiota composition and epithelial barrier stability. Several nutrients have already been associated with a protective phenotype. Yet, there is a lack of knowledge toward the most promising ones as well as the most adequate phase of action. To unveil the most prominent therapy candidates we characterized the colon metabolic profile during colitis development. We have observed a twofold decrease in threonine levels in mice subjected to DSS-induced colitis. We then assessed the effect of threonine supplementation in the beginning of the inflammatory process (DSS + Thr) or when inflammation is already established (DSS + Thr D8). Colitis progression was similar between the treated groups and control colitic mice, yet threonine had a surprisingly detrimental effect when administered in the beginning of the disease, with mice displaying a delayed recovery when compared to control mice and mice supplemented with threonine after day 8. Although no major changes were found in their metabolic profile, DSS + Thr mice displayed altered expression in mucin-encoding genes, as well as in goblet cell counts, unveiling an impaired ability to produce mucus. Moreover, IL-22 secretion was decreased in DSS + Thr mice when compared to DSS + Thr D8 mice. Overall, these results suggest that supplementation with threonine during colitis induction impact goblet cell number and delays the recovery period. This reinforces the importance of a deeper understanding regarding threonine supplementation in IBD. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-09-05 2018-09-05T00:00:00Z 2019-04-26T22:14:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.3389/fphys.2018.01247 |
url |
https://doi.org/10.3389/fphys.2018.01247 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1664-042X PURE: 12428541 http://www.scopus.com/inward/record.url?scp=85053078274&partnerID=8YFLogxK https://doi.org/10.3389/fphys.2018.01247 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799137968189865984 |