Data Descriptor: Mountain hare transcriptome and diagnostic markers as resources to monitor hybridization with European hares

Detalhes bibliográficos
Autor(a) principal: Marques, João P.
Data de Publicação: 2017
Outros Autores: Ferreira, Mafalda, S., Farelo, Liliana, Callahan, Colin M., Hacklander, Klaus, Jenny, Hannes, Montgomery, W. Ian, Reid, Neil, Good, Jeffrey M., Alves, Paulo C., Melo-Ferreira, José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/10216/110951
Resumo: We report the first mountain hare (Lepus timidus) transcriptome, produced by de novo assembly of RNA-sequencing reads. Data were obtained from eight specimens sampled in two localities, Alps and Ireland. The mountain hare tends to be replaced by the invading European hare (Lepus europaeus) in their numerous contact zones where the species hybridize, which affects their gene pool to a yet unquantified degree. We characterize and annotate the mountain hare transcriptome, detect polymorphism in the two analysed populations and use previously published data on the European hare (three specimens, representing the European lineage of the species) to identify 4 672 putative diagnostic sites between the species. A subset of 85 random independent SNPs was successfully validated using PCR and Sanger sequencing. These valuable genomic resources can be used to design tools to assess population status and monitor hybridization between species.
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spelling Data Descriptor: Mountain hare transcriptome and diagnostic markers as resources to monitor hybridization with European haresWe report the first mountain hare (Lepus timidus) transcriptome, produced by de novo assembly of RNA-sequencing reads. Data were obtained from eight specimens sampled in two localities, Alps and Ireland. The mountain hare tends to be replaced by the invading European hare (Lepus europaeus) in their numerous contact zones where the species hybridize, which affects their gene pool to a yet unquantified degree. We characterize and annotate the mountain hare transcriptome, detect polymorphism in the two analysed populations and use previously published data on the European hare (three specimens, representing the European lineage of the species) to identify 4 672 putative diagnostic sites between the species. A subset of 85 random independent SNPs was successfully validated using PCR and Sanger sequencing. These valuable genomic resources can be used to design tools to assess population status and monitor hybridization between species.2017-12-052017-12-05T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/110951eng10.1038/sdata.2017.178Marques, João P.Ferreira, Mafalda, S.Farelo, LilianaCallahan, Colin M.Hacklander, KlausJenny, HannesMontgomery, W. IanReid, NeilGood, Jeffrey M.Alves, Paulo C.Melo-Ferreira, Joséinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T14:05:45Zoai:repositorio-aberto.up.pt:10216/110951Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:54:39.351584Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Data Descriptor: Mountain hare transcriptome and diagnostic markers as resources to monitor hybridization with European hares
title Data Descriptor: Mountain hare transcriptome and diagnostic markers as resources to monitor hybridization with European hares
spellingShingle Data Descriptor: Mountain hare transcriptome and diagnostic markers as resources to monitor hybridization with European hares
Marques, João P.
title_short Data Descriptor: Mountain hare transcriptome and diagnostic markers as resources to monitor hybridization with European hares
title_full Data Descriptor: Mountain hare transcriptome and diagnostic markers as resources to monitor hybridization with European hares
title_fullStr Data Descriptor: Mountain hare transcriptome and diagnostic markers as resources to monitor hybridization with European hares
title_full_unstemmed Data Descriptor: Mountain hare transcriptome and diagnostic markers as resources to monitor hybridization with European hares
title_sort Data Descriptor: Mountain hare transcriptome and diagnostic markers as resources to monitor hybridization with European hares
author Marques, João P.
author_facet Marques, João P.
Ferreira, Mafalda, S.
Farelo, Liliana
Callahan, Colin M.
Hacklander, Klaus
Jenny, Hannes
Montgomery, W. Ian
Reid, Neil
Good, Jeffrey M.
Alves, Paulo C.
Melo-Ferreira, José
author_role author
author2 Ferreira, Mafalda, S.
Farelo, Liliana
Callahan, Colin M.
Hacklander, Klaus
Jenny, Hannes
Montgomery, W. Ian
Reid, Neil
Good, Jeffrey M.
Alves, Paulo C.
Melo-Ferreira, José
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Marques, João P.
Ferreira, Mafalda, S.
Farelo, Liliana
Callahan, Colin M.
Hacklander, Klaus
Jenny, Hannes
Montgomery, W. Ian
Reid, Neil
Good, Jeffrey M.
Alves, Paulo C.
Melo-Ferreira, José
description We report the first mountain hare (Lepus timidus) transcriptome, produced by de novo assembly of RNA-sequencing reads. Data were obtained from eight specimens sampled in two localities, Alps and Ireland. The mountain hare tends to be replaced by the invading European hare (Lepus europaeus) in their numerous contact zones where the species hybridize, which affects their gene pool to a yet unquantified degree. We characterize and annotate the mountain hare transcriptome, detect polymorphism in the two analysed populations and use previously published data on the European hare (three specimens, representing the European lineage of the species) to identify 4 672 putative diagnostic sites between the species. A subset of 85 random independent SNPs was successfully validated using PCR and Sanger sequencing. These valuable genomic resources can be used to design tools to assess population status and monitor hybridization between species.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-05
2017-12-05T00:00:00Z
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language eng
dc.relation.none.fl_str_mv 10.1038/sdata.2017.178
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