Agrobacterium cavarae sp. nov., isolated from maize (Zea mays L.) roots
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/31906 https://doi.org/10.1099/ijsem.0.004441 |
Resumo: | A bacterial strain designated as RZME10T was isolated from a Zea mays L. root collected in Spain. Results of analysis of the 16S rRNA gene sequence showed that this strain belongs to the genus Agrobacterium with Agrobacterium larrymoorei ATCC 51759T being the most closely related species with 99.9 % sequence similarity. The similarity values of the rpoB, recA, gyrB, atpD and glnII genes between strain RZME10T and A. larrymoorei ATCC 51759T were 93.5, 90.0, 88.7, 87.9 and 90.1 %, respectively. The estimated average nucleotide identity using blast and digital DNA–DNA hybridization values between these two strains were 80.4 and 30.2 %, respectively. The major fatty acids of strain RZME10T are those from summed feature 8 (C18 : 1 ω6c/C18 : 1 ω7c) and C16 : 0. Pathogenicity tests on tomato and carrot roots showed that strain RZME10T was not able to induce plant tumours. Based on the results of genomic, chemotaxonomic and phenotypic analyses, we propose that strain RZME10T represents a novel species named Agrobacterium cavarae sp. nov. (type strain RZME10T=CECT 9795T=LMG 31257T). |
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Agrobacterium cavarae sp. nov., isolated from maize (Zea mays L.) rootsA bacterial strain designated as RZME10T was isolated from a Zea mays L. root collected in Spain. Results of analysis of the 16S rRNA gene sequence showed that this strain belongs to the genus Agrobacterium with Agrobacterium larrymoorei ATCC 51759T being the most closely related species with 99.9 % sequence similarity. The similarity values of the rpoB, recA, gyrB, atpD and glnII genes between strain RZME10T and A. larrymoorei ATCC 51759T were 93.5, 90.0, 88.7, 87.9 and 90.1 %, respectively. The estimated average nucleotide identity using blast and digital DNA–DNA hybridization values between these two strains were 80.4 and 30.2 %, respectively. The major fatty acids of strain RZME10T are those from summed feature 8 (C18 : 1 ω6c/C18 : 1 ω7c) and C16 : 0. Pathogenicity tests on tomato and carrot roots showed that strain RZME10T was not able to induce plant tumours. Based on the results of genomic, chemotaxonomic and phenotypic analyses, we propose that strain RZME10T represents a novel species named Agrobacterium cavarae sp. nov. (type strain RZME10T=CECT 9795T=LMG 31257T).Microbiology Society2022-04-27T15:06:25Z2022-04-272020-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/31906http://hdl.handle.net/10174/31906https://doi.org/10.1099/ijsem.0.004441porFlores-Félix, J. D., Menéndez, E., Ramírez-Bahena, M. H., Peix, A., García-Fraile, P., & Velázquez, E. (2020). Agrobacterium cavarae sp. nov., isolated from maize (Zea mays L.) roots. International Journal of Systematic and Evolutionary Microbiology, 70(10), 5512-5519.ndesthermenendez@uevora.ptndndndndFlores-Felix, Jose DMenendez, EstherRamirez-Bahena, MartaHPeix, AlvaroGarcia-Fraile, PaulaVelázquez, Encarnainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:31:21Zoai:dspace.uevora.pt:10174/31906Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:20:42.953079Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Agrobacterium cavarae sp. nov., isolated from maize (Zea mays L.) roots |
title |
Agrobacterium cavarae sp. nov., isolated from maize (Zea mays L.) roots |
spellingShingle |
Agrobacterium cavarae sp. nov., isolated from maize (Zea mays L.) roots Flores-Felix, Jose D |
title_short |
Agrobacterium cavarae sp. nov., isolated from maize (Zea mays L.) roots |
title_full |
Agrobacterium cavarae sp. nov., isolated from maize (Zea mays L.) roots |
title_fullStr |
Agrobacterium cavarae sp. nov., isolated from maize (Zea mays L.) roots |
title_full_unstemmed |
Agrobacterium cavarae sp. nov., isolated from maize (Zea mays L.) roots |
title_sort |
Agrobacterium cavarae sp. nov., isolated from maize (Zea mays L.) roots |
author |
Flores-Felix, Jose D |
author_facet |
Flores-Felix, Jose D Menendez, Esther Ramirez-Bahena, MartaH Peix, Alvaro Garcia-Fraile, Paula Velázquez, Encarna |
author_role |
author |
author2 |
Menendez, Esther Ramirez-Bahena, MartaH Peix, Alvaro Garcia-Fraile, Paula Velázquez, Encarna |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Flores-Felix, Jose D Menendez, Esther Ramirez-Bahena, MartaH Peix, Alvaro Garcia-Fraile, Paula Velázquez, Encarna |
description |
A bacterial strain designated as RZME10T was isolated from a Zea mays L. root collected in Spain. Results of analysis of the 16S rRNA gene sequence showed that this strain belongs to the genus Agrobacterium with Agrobacterium larrymoorei ATCC 51759T being the most closely related species with 99.9 % sequence similarity. The similarity values of the rpoB, recA, gyrB, atpD and glnII genes between strain RZME10T and A. larrymoorei ATCC 51759T were 93.5, 90.0, 88.7, 87.9 and 90.1 %, respectively. The estimated average nucleotide identity using blast and digital DNA–DNA hybridization values between these two strains were 80.4 and 30.2 %, respectively. The major fatty acids of strain RZME10T are those from summed feature 8 (C18 : 1 ω6c/C18 : 1 ω7c) and C16 : 0. Pathogenicity tests on tomato and carrot roots showed that strain RZME10T was not able to induce plant tumours. Based on the results of genomic, chemotaxonomic and phenotypic analyses, we propose that strain RZME10T represents a novel species named Agrobacterium cavarae sp. nov. (type strain RZME10T=CECT 9795T=LMG 31257T). |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-01T00:00:00Z 2022-04-27T15:06:25Z 2022-04-27 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/31906 http://hdl.handle.net/10174/31906 https://doi.org/10.1099/ijsem.0.004441 |
url |
http://hdl.handle.net/10174/31906 https://doi.org/10.1099/ijsem.0.004441 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Flores-Félix, J. D., Menéndez, E., Ramírez-Bahena, M. H., Peix, A., García-Fraile, P., & Velázquez, E. (2020). Agrobacterium cavarae sp. nov., isolated from maize (Zea mays L.) roots. International Journal of Systematic and Evolutionary Microbiology, 70(10), 5512-5519. nd esthermenendez@uevora.pt nd nd nd nd |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Microbiology Society |
publisher.none.fl_str_mv |
Microbiology Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136688907223040 |