Unveiling the Toxicity of Fine and Nano-Sized Airborne Particles Generated from Industrial Thermal Spraying Processes in Human Alveolar Epithelial Cells
| Autor(a) principal: | |
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| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.18/8451 |
Resumo: | This article belongs to the Special Issue Toxicity of Nanomaterials and Legacy Contaminants: Risks to the Environment and Human Health. |
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Unveiling the Toxicity of Fine and Nano-Sized Airborne Particles Generated from Industrial Thermal Spraying Processes in Human Alveolar Epithelial CellsA549Cell CycleCytotoxicityDNA DamageIn vitro ToxicityIncidental NanoparticlesProcess-generated NanoparticelsOccupational ExposureToxicologiaAr e Saúde OcupacionalThis article belongs to the Special Issue Toxicity of Nanomaterials and Legacy Contaminants: Risks to the Environment and Human Health.High-energy industrial processes have been associated with particle release into workplace air that can adversely affect workers’ health. The present study assessed the toxicity of incidental fine (PGFP) and nanoparticles (PGNP) emitted from atmospheric plasma (APS) and high-velocity oxy-fuel (HVOF) thermal spraying. Lactate dehydrogenase (LDH) release, 2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate (WST-1) metabolisation, intracellular reactive oxygen species (ROS) levels, cell cycle changes, histone H2AX phosphorylation (γ-H2AX) and DNA damage were evaluated in human alveolar epithelial cells at 24 h after exposure. Overall, HVOF particles were the most cytotoxic to human alveolar cells, with cell viability half-maximal inhibitory concentration (IC50) values of 20.18 µg/cm2 and 1.79 µg/cm2 for PGFP and PGNP, respectively. Only the highest tested concentration of APS-PGFP caused a slight decrease in cell viability. Particle uptake, cell cycle arrest at S + G2/M and γ-H2AX augmentation were observed after exposure to all tested particles. However, higher levels of γ-H2AX were found in cells exposed to APS-derived particles (~16%), while cells exposed to HVOF particles exhibited increased levels of oxidative damage (~17% tail intensity) and ROS (~184%). Accordingly, APS and HVOF particles seem to exert their genotoxic effects by different mechanisms, highlighting that the health risks of these process-generated particles at industrial settings should not be underestimated.This research was funded by the project CERASAFE with the support of ERA-NET SIINN (project id:16) and the Portuguese Foundation for Science and Technology (FCT; SIINN/0004/2014). This work was also supported by the project NanoBioBarriers (PTDC/MED-TOX/31162/2017), co financed by the Operational Program for Competitiveness and Internationalization (POCI) through European Regional Development Funds (FEDER/FNR) and FCT and the Spanish Ministry of Science and Innovation (projects PCIN-2015-173-C02-01 and CEX2018-000794-S-Severo Ochoa). M.J. Bessa (SFRH/BD/120646/2016) and F. Brandão (SFRH/BD/101060/2014) are recipients of FCT PhD scholarships under the framework of the Human Capital Operating Program (POCH) and European Union funding. The Doctoral Program in Biomedical Sciences of the ICBAS—University of Porto offered additional funds. S. Fraga thanks FCT for funding through program DL 57/2016—Norma transitória (Ref. DL-57/INSA-06/2018). Thanks are also due to FCT/MCTES for the financial support to EPIUnit and ITR (UIDB/04750/2020 and LA/P/0064/2020).MDPIRepositório Científico do Instituto Nacional de SaúdeBessa, Maria JoãoBrandão, FátimaFokkens, Paul H.B.Leseman, Daan L.A.C.Boere, A. John F.Cassee, Flemming R.Salmatonidis, ApostolosViana, MarMonfort, EliseoFraga, SóniaTeixeira, João Paulo2023-01-25T10:58:31Z2022-04-132022-04-13T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8451engInt J Mol Sci. 2022 Apr 13;23(8):4278. doi: 10.3390/ijms230842781661-659610.3390/ijms23084278info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:29Zoai:repositorio.insa.pt:10400.18/8451Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:57.124885Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Unveiling the Toxicity of Fine and Nano-Sized Airborne Particles Generated from Industrial Thermal Spraying Processes in Human Alveolar Epithelial Cells |
| title |
Unveiling the Toxicity of Fine and Nano-Sized Airborne Particles Generated from Industrial Thermal Spraying Processes in Human Alveolar Epithelial Cells |
| spellingShingle |
Unveiling the Toxicity of Fine and Nano-Sized Airborne Particles Generated from Industrial Thermal Spraying Processes in Human Alveolar Epithelial Cells Bessa, Maria João A549 Cell Cycle Cytotoxicity DNA Damage In vitro Toxicity Incidental Nanoparticles Process-generated Nanoparticels Occupational Exposure Toxicologia Ar e Saúde Ocupacional |
| title_short |
Unveiling the Toxicity of Fine and Nano-Sized Airborne Particles Generated from Industrial Thermal Spraying Processes in Human Alveolar Epithelial Cells |
| title_full |
Unveiling the Toxicity of Fine and Nano-Sized Airborne Particles Generated from Industrial Thermal Spraying Processes in Human Alveolar Epithelial Cells |
| title_fullStr |
Unveiling the Toxicity of Fine and Nano-Sized Airborne Particles Generated from Industrial Thermal Spraying Processes in Human Alveolar Epithelial Cells |
| title_full_unstemmed |
Unveiling the Toxicity of Fine and Nano-Sized Airborne Particles Generated from Industrial Thermal Spraying Processes in Human Alveolar Epithelial Cells |
| title_sort |
Unveiling the Toxicity of Fine and Nano-Sized Airborne Particles Generated from Industrial Thermal Spraying Processes in Human Alveolar Epithelial Cells |
| author |
Bessa, Maria João |
| author_facet |
Bessa, Maria João Brandão, Fátima Fokkens, Paul H.B. Leseman, Daan L.A.C. Boere, A. John F. Cassee, Flemming R. Salmatonidis, Apostolos Viana, Mar Monfort, Eliseo Fraga, Sónia Teixeira, João Paulo |
| author_role |
author |
| author2 |
Brandão, Fátima Fokkens, Paul H.B. Leseman, Daan L.A.C. Boere, A. John F. Cassee, Flemming R. Salmatonidis, Apostolos Viana, Mar Monfort, Eliseo Fraga, Sónia Teixeira, João Paulo |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
| dc.contributor.author.fl_str_mv |
Bessa, Maria João Brandão, Fátima Fokkens, Paul H.B. Leseman, Daan L.A.C. Boere, A. John F. Cassee, Flemming R. Salmatonidis, Apostolos Viana, Mar Monfort, Eliseo Fraga, Sónia Teixeira, João Paulo |
| dc.subject.por.fl_str_mv |
A549 Cell Cycle Cytotoxicity DNA Damage In vitro Toxicity Incidental Nanoparticles Process-generated Nanoparticels Occupational Exposure Toxicologia Ar e Saúde Ocupacional |
| topic |
A549 Cell Cycle Cytotoxicity DNA Damage In vitro Toxicity Incidental Nanoparticles Process-generated Nanoparticels Occupational Exposure Toxicologia Ar e Saúde Ocupacional |
| description |
This article belongs to the Special Issue Toxicity of Nanomaterials and Legacy Contaminants: Risks to the Environment and Human Health. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-04-13 2022-04-13T00:00:00Z 2023-01-25T10:58:31Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10400.18/8451 |
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http://hdl.handle.net/10400.18/8451 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
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Int J Mol Sci. 2022 Apr 13;23(8):4278. doi: 10.3390/ijms23084278 1661-6596 10.3390/ijms23084278 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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MDPI |
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MDPI |
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