Parallel Propagation of Toxoplasma gondii In Vivo, In Vitro and in Alternate Model: Towards Less Dependence on the Mice Model

Detalhes bibliográficos
Autor(a) principal: Gargaté, Maria João
Data de Publicação: 2022
Outros Autores: Vilares, Anabela, Ferreira, Idalina, Reis, Tânia, Martins, Susana, Mendonça, Joana, Borges, Vítor, Gomes, João Paulo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/8468
Resumo: This article belongs to the Special Issue Epidemiology and Management of Foodborne Parasitic Diseases.
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spelling Parallel Propagation of Toxoplasma gondii In Vivo, In Vitro and in Alternate Model: Towards Less Dependence on the Mice Model3RsToxoplasma gondiiIn vitro PropagationMice ModelStrain PropagationVirulenceInfecções Sistémicas e ZoonosesThis article belongs to the Special Issue Epidemiology and Management of Foodborne Parasitic Diseases.Toxoplasma gondii is an obligate intracellular protozoan. In pregnant women, it can lead to severe birth defects or intrauterine death of the fetus. Most of what is currently know on cell biology of T. gondii comes from studies relying on the RH strain propagated in mice. According to the recommendations concerning the animal welfare, we assayed in vitro/in vivo procedures to replace, or at least reduce, the demanding animal model for strain propagation. We evaluated the genetic and phenotypic stability of the RH strain throughout its parallel continuous propagation in mice, in human foreskin fibroblasts (HFF) and in an alternate fashion of these two procedures. We also assessed the virulence impact on the RH strain after different periods of its long-term propagation strictly in cells. The RH strain completely lost its virulence after long-term passage in HFF. Nevertheless, we obtained a successful outcome with the alternate passaging of the parasite in HFF and in mice as this approach enabled T. gondii to maintain the evaluated phenotypic properties, mainly its virulence potential. Also, no genetic changes were observed in genes known to be highly polymorphic or involved in pathoadaptation. In conclusion, the alternate model seems to be a feasible method for T. gondii propagation and maintenance, strongly impacting the number of sacrificed mice.This research received no external funding. This study was funded by Infectious Diseases Department of National Institute of Health (NIH) Dr Ricardo Jorge, Portugal.MDPIRepositório Científico do Instituto Nacional de SaúdeGargaté, Maria JoãoVilares, AnabelaFerreira, IdalinaReis, TâniaMartins, SusanaMendonça, JoanaBorges, VítorGomes, João Paulo2023-01-30T11:39:41Z2022-09-132022-09-13T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8468engPathogens. 2022 Sep 13;11(9):1038. doi: 10.3390/pathogens11091038.2076-081710.3390/pathogens11091038info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:35Zoai:repositorio.insa.pt:10400.18/8468Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:43:06.761617Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Parallel Propagation of Toxoplasma gondii In Vivo, In Vitro and in Alternate Model: Towards Less Dependence on the Mice Model
title Parallel Propagation of Toxoplasma gondii In Vivo, In Vitro and in Alternate Model: Towards Less Dependence on the Mice Model
spellingShingle Parallel Propagation of Toxoplasma gondii In Vivo, In Vitro and in Alternate Model: Towards Less Dependence on the Mice Model
Gargaté, Maria João
3Rs
Toxoplasma gondii
In vitro Propagation
Mice Model
Strain Propagation
Virulence
Infecções Sistémicas e Zoonoses
title_short Parallel Propagation of Toxoplasma gondii In Vivo, In Vitro and in Alternate Model: Towards Less Dependence on the Mice Model
title_full Parallel Propagation of Toxoplasma gondii In Vivo, In Vitro and in Alternate Model: Towards Less Dependence on the Mice Model
title_fullStr Parallel Propagation of Toxoplasma gondii In Vivo, In Vitro and in Alternate Model: Towards Less Dependence on the Mice Model
title_full_unstemmed Parallel Propagation of Toxoplasma gondii In Vivo, In Vitro and in Alternate Model: Towards Less Dependence on the Mice Model
title_sort Parallel Propagation of Toxoplasma gondii In Vivo, In Vitro and in Alternate Model: Towards Less Dependence on the Mice Model
author Gargaté, Maria João
author_facet Gargaté, Maria João
Vilares, Anabela
Ferreira, Idalina
Reis, Tânia
Martins, Susana
Mendonça, Joana
Borges, Vítor
Gomes, João Paulo
author_role author
author2 Vilares, Anabela
Ferreira, Idalina
Reis, Tânia
Martins, Susana
Mendonça, Joana
Borges, Vítor
Gomes, João Paulo
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Gargaté, Maria João
Vilares, Anabela
Ferreira, Idalina
Reis, Tânia
Martins, Susana
Mendonça, Joana
Borges, Vítor
Gomes, João Paulo
dc.subject.por.fl_str_mv 3Rs
Toxoplasma gondii
In vitro Propagation
Mice Model
Strain Propagation
Virulence
Infecções Sistémicas e Zoonoses
topic 3Rs
Toxoplasma gondii
In vitro Propagation
Mice Model
Strain Propagation
Virulence
Infecções Sistémicas e Zoonoses
description This article belongs to the Special Issue Epidemiology and Management of Foodborne Parasitic Diseases.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-13
2022-09-13T00:00:00Z
2023-01-30T11:39:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/8468
url http://hdl.handle.net/10400.18/8468
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pathogens. 2022 Sep 13;11(9):1038. doi: 10.3390/pathogens11091038.
2076-0817
10.3390/pathogens11091038
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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