Hierarchical HRP-crosslinked silk fibroin/ZnSr-TCP scaffolds for osteochondral tissue regeneration: assessment of the mechanical and antibacterial properties

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Viviana Pinto
Data de Publicação: 2020
Outros Autores: Pina, Sandra Cristina Almeida, Gheduzzi, Sabina, Araújo, Ana C., Reis, R. L., Oliveira, Joaquim M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/64351
Resumo: "Article 49"
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spelling Hierarchical HRP-crosslinked silk fibroin/ZnSr-TCP scaffolds for osteochondral tissue regeneration: assessment of the mechanical and antibacterial propertiesSilk fibroinHorseradish peroxidaseZnSr-tricalcium phosphateHierarchical scaffoldsMechanical strengthAntibacterial adhesionOsteochondral regenerationScience & Technology"Article 49"The biomaterials requirements for osteochondral (OC) defects restoration simultaneously include adequate mechanical behavior, and the prevention of bacterial adherence and biofilm formation, without impairing local tissue integration. Bilayered and hierarchical scaffolds combining a cartilage-like layer interconnected to an underlying subchondral bone-like layer appeared as innovative technological solutions able to mimic the native OC tissue hierarchical architecture. This study is focused on the assessment of the combined compression-shear stresses and possible bacterial biofilm formation of hierarchical scaffolds prepared from a horseradish peroxidase-crosslinking reaction of silk fibroin (SF) combined with zinc (Zn) and strontium (Sr)-doped β-tricalcium phosphate (β-TCP) for OC tissue regeneration. Scaffolds with undoped-β-TCP incorporation were used as control. Results showed that the bilayered scaffolds presented suitable aptitude to support compression and shear loading for OC tissue, with better mechanical properties for the ZnSr-containing structures. Young and shear moduli presented values close to 0.01 MPa in the region 10â 20% strain. The investigation of biomaterials surface ability to prevent biofilm formation showed reduced bacterial adhesion of Escherichia coli (E. coli, gram-negative) and Staphylococcus aureus (S. aureus, gram-positive) on both scaffolds, thus suggesting that the proposed hierarchical scaffolds have a positive effect in preventing gram-positive and gram-negative bacteria proliferation.This research was funded by the Portuguese Foundation for Science and Technology for the Hierarchitech project (M-era-Net/0001/2014), and by the Research and Innovation Staff Exchanges (RISE) action (H2020 Marie Skłodowska-Curie actions) for the BAMOS project (H2020-MSCA-RISE-2016- 734156). The authors also thank the funds provided under the distinctions attributed to JO (IF/01285/2015) and SP (CEECIND/03673/2017).Frontiers MediaUniversidade do MinhoRibeiro, Viviana PintoPina, Sandra Cristina AlmeidaGheduzzi, SabinaAraújo, Ana C.Reis, R. L.Oliveira, Joaquim M.2020-032020-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/64351engRibeiro V. P., Pina S., Gheduzzi S., Araújo A. C., Reis R. L., Oliveira J. M. Hierarchical HRP-Crosslinked Silk Fibroin/ZnSr-TCP Scaffolds for Osteochondral Tissue Regeneration: Assessment of the Mechanical and Antibacterial Properties, Frontiers in Materials, Vol. 7, doi:10.3389/fmats.2020.00049, 20202296-80162296-801610.3389/fmats.2020.00049https://www.frontiersin.org/articles/10.3389/fmats.2020.00049/fullinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:18:06Zoai:repositorium.sdum.uminho.pt:1822/64351Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:10:50.242184Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Hierarchical HRP-crosslinked silk fibroin/ZnSr-TCP scaffolds for osteochondral tissue regeneration: assessment of the mechanical and antibacterial properties
title Hierarchical HRP-crosslinked silk fibroin/ZnSr-TCP scaffolds for osteochondral tissue regeneration: assessment of the mechanical and antibacterial properties
spellingShingle Hierarchical HRP-crosslinked silk fibroin/ZnSr-TCP scaffolds for osteochondral tissue regeneration: assessment of the mechanical and antibacterial properties
Ribeiro, Viviana Pinto
Silk fibroin
Horseradish peroxidase
ZnSr-tricalcium phosphate
Hierarchical scaffolds
Mechanical strength
Antibacterial adhesion
Osteochondral regeneration
Science & Technology
title_short Hierarchical HRP-crosslinked silk fibroin/ZnSr-TCP scaffolds for osteochondral tissue regeneration: assessment of the mechanical and antibacterial properties
title_full Hierarchical HRP-crosslinked silk fibroin/ZnSr-TCP scaffolds for osteochondral tissue regeneration: assessment of the mechanical and antibacterial properties
title_fullStr Hierarchical HRP-crosslinked silk fibroin/ZnSr-TCP scaffolds for osteochondral tissue regeneration: assessment of the mechanical and antibacterial properties
title_full_unstemmed Hierarchical HRP-crosslinked silk fibroin/ZnSr-TCP scaffolds for osteochondral tissue regeneration: assessment of the mechanical and antibacterial properties
title_sort Hierarchical HRP-crosslinked silk fibroin/ZnSr-TCP scaffolds for osteochondral tissue regeneration: assessment of the mechanical and antibacterial properties
author Ribeiro, Viviana Pinto
author_facet Ribeiro, Viviana Pinto
Pina, Sandra Cristina Almeida
Gheduzzi, Sabina
Araújo, Ana C.
Reis, R. L.
Oliveira, Joaquim M.
author_role author
author2 Pina, Sandra Cristina Almeida
Gheduzzi, Sabina
Araújo, Ana C.
Reis, R. L.
Oliveira, Joaquim M.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Ribeiro, Viviana Pinto
Pina, Sandra Cristina Almeida
Gheduzzi, Sabina
Araújo, Ana C.
Reis, R. L.
Oliveira, Joaquim M.
dc.subject.por.fl_str_mv Silk fibroin
Horseradish peroxidase
ZnSr-tricalcium phosphate
Hierarchical scaffolds
Mechanical strength
Antibacterial adhesion
Osteochondral regeneration
Science & Technology
topic Silk fibroin
Horseradish peroxidase
ZnSr-tricalcium phosphate
Hierarchical scaffolds
Mechanical strength
Antibacterial adhesion
Osteochondral regeneration
Science & Technology
description "Article 49"
publishDate 2020
dc.date.none.fl_str_mv 2020-03
2020-03-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/64351
url http://hdl.handle.net/1822/64351
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ribeiro V. P., Pina S., Gheduzzi S., Araújo A. C., Reis R. L., Oliveira J. M. Hierarchical HRP-Crosslinked Silk Fibroin/ZnSr-TCP Scaffolds for Osteochondral Tissue Regeneration: Assessment of the Mechanical and Antibacterial Properties, Frontiers in Materials, Vol. 7, doi:10.3389/fmats.2020.00049, 2020
2296-8016
2296-8016
10.3389/fmats.2020.00049
https://www.frontiersin.org/articles/10.3389/fmats.2020.00049/full
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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