Assessment of markers of bone formation under controlled environmental factors and their correlation with serum minerals in adult sheep as a model for orthopaedic research
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10348/1467 |
Resumo: | Eighteen healthy skeletally mature (3 years old) ewes, with an average weight of 45 kg, of the Portuguese Churra da Terra Quente breed were used to evaluate the normal values of total and bone-specific isoform of alkaline phosphatase serum activities (ALP and BALP, respectively) and serum osteocalcin (OC) and their correlation with the serum minerals – calcium (Ca), phosphorus (P), magnesium (Mg) and ionized calcium (Ca2þ). The sheep were maintained under controlled environmental conditions (constant diurnal photoperiod cycle and identical husbandry and feeding) for six weeks before the collection of the blood samples. The measurement of the total ALP and serum minerals was performed with automated biochemistry analysers using the BioMe´rieuxw kits, the serum electrolyte Ca2þ Diametrics Medical, Incw specific cassettes and the BALP and OC METRATM kits from QUIDELw Corporation. The mean+standard deviation values obtained were: total ALP 90.17+ 85.72 U/L, BALP 15.0+5.44 U/L, ratio BALP/ total ALP 29.28+24.22, OC 13.02+ 1.87 ng/mL, Ca 2.57+0.37 mmol/L, P 2.13+0.42 mmol/L, Mg 1.04+0.13 mmol/L, Ca2þ 1.29+0.04 mmol/L. Significant correlations were observed between the total ALP and Ca (r ¼ 0.5939; P ¼ 0.05) and OC and Ca (r ¼ 0.5706; P ¼ 0.05). Reference to the serum values of bone turnover parameters in sheep could be of great value in research and could provide complementary non-invasive information on the bone healing process, particularly with regard to obtaining an early prognosis of fracture healing. |
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Assessment of markers of bone formation under controlled environmental factors and their correlation with serum minerals in adult sheep as a model for orthopaedic researchtotal and bone-specific alkaline phosphataseosteocalcinmineralsserumsheepEighteen healthy skeletally mature (3 years old) ewes, with an average weight of 45 kg, of the Portuguese Churra da Terra Quente breed were used to evaluate the normal values of total and bone-specific isoform of alkaline phosphatase serum activities (ALP and BALP, respectively) and serum osteocalcin (OC) and their correlation with the serum minerals – calcium (Ca), phosphorus (P), magnesium (Mg) and ionized calcium (Ca2þ). The sheep were maintained under controlled environmental conditions (constant diurnal photoperiod cycle and identical husbandry and feeding) for six weeks before the collection of the blood samples. The measurement of the total ALP and serum minerals was performed with automated biochemistry analysers using the BioMe´rieuxw kits, the serum electrolyte Ca2þ Diametrics Medical, Incw specific cassettes and the BALP and OC METRATM kits from QUIDELw Corporation. The mean+standard deviation values obtained were: total ALP 90.17+ 85.72 U/L, BALP 15.0+5.44 U/L, ratio BALP/ total ALP 29.28+24.22, OC 13.02+ 1.87 ng/mL, Ca 2.57+0.37 mmol/L, P 2.13+0.42 mmol/L, Mg 1.04+0.13 mmol/L, Ca2þ 1.29+0.04 mmol/L. Significant correlations were observed between the total ALP and Ca (r ¼ 0.5939; P ¼ 0.05) and OC and Ca (r ¼ 0.5706; P ¼ 0.05). Reference to the serum values of bone turnover parameters in sheep could be of great value in research and could provide complementary non-invasive information on the bone healing process, particularly with regard to obtaining an early prognosis of fracture healing.2011-12-28T10:17:33Z2008-01-01T00:00:00Z2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10348/1467engmetadata only accessinfo:eu-repo/semantics/openAccessDias, Maria Isabel R.Viegas, Carlos A.Azevedo, Jorge Manuel Teixeira deCosta, E. M.Lourenço, P.Rodrigues, A.Cabrita, António M. Silvérioreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-02T12:57:05Zoai:repositorio.utad.pt:10348/1467Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:06:34.774880Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Assessment of markers of bone formation under controlled environmental factors and their correlation with serum minerals in adult sheep as a model for orthopaedic research |
title |
Assessment of markers of bone formation under controlled environmental factors and their correlation with serum minerals in adult sheep as a model for orthopaedic research |
spellingShingle |
Assessment of markers of bone formation under controlled environmental factors and their correlation with serum minerals in adult sheep as a model for orthopaedic research Dias, Maria Isabel R. total and bone-specific alkaline phosphatase osteocalcin minerals serum sheep |
title_short |
Assessment of markers of bone formation under controlled environmental factors and their correlation with serum minerals in adult sheep as a model for orthopaedic research |
title_full |
Assessment of markers of bone formation under controlled environmental factors and their correlation with serum minerals in adult sheep as a model for orthopaedic research |
title_fullStr |
Assessment of markers of bone formation under controlled environmental factors and their correlation with serum minerals in adult sheep as a model for orthopaedic research |
title_full_unstemmed |
Assessment of markers of bone formation under controlled environmental factors and their correlation with serum minerals in adult sheep as a model for orthopaedic research |
title_sort |
Assessment of markers of bone formation under controlled environmental factors and their correlation with serum minerals in adult sheep as a model for orthopaedic research |
author |
Dias, Maria Isabel R. |
author_facet |
Dias, Maria Isabel R. Viegas, Carlos A. Azevedo, Jorge Manuel Teixeira de Costa, E. M. Lourenço, P. Rodrigues, A. Cabrita, António M. Silvério |
author_role |
author |
author2 |
Viegas, Carlos A. Azevedo, Jorge Manuel Teixeira de Costa, E. M. Lourenço, P. Rodrigues, A. Cabrita, António M. Silvério |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Dias, Maria Isabel R. Viegas, Carlos A. Azevedo, Jorge Manuel Teixeira de Costa, E. M. Lourenço, P. Rodrigues, A. Cabrita, António M. Silvério |
dc.subject.por.fl_str_mv |
total and bone-specific alkaline phosphatase osteocalcin minerals serum sheep |
topic |
total and bone-specific alkaline phosphatase osteocalcin minerals serum sheep |
description |
Eighteen healthy skeletally mature (3 years old) ewes, with an average weight of 45 kg, of the Portuguese Churra da Terra Quente breed were used to evaluate the normal values of total and bone-specific isoform of alkaline phosphatase serum activities (ALP and BALP, respectively) and serum osteocalcin (OC) and their correlation with the serum minerals – calcium (Ca), phosphorus (P), magnesium (Mg) and ionized calcium (Ca2þ). The sheep were maintained under controlled environmental conditions (constant diurnal photoperiod cycle and identical husbandry and feeding) for six weeks before the collection of the blood samples. The measurement of the total ALP and serum minerals was performed with automated biochemistry analysers using the BioMe´rieuxw kits, the serum electrolyte Ca2þ Diametrics Medical, Incw specific cassettes and the BALP and OC METRATM kits from QUIDELw Corporation. The mean+standard deviation values obtained were: total ALP 90.17+ 85.72 U/L, BALP 15.0+5.44 U/L, ratio BALP/ total ALP 29.28+24.22, OC 13.02+ 1.87 ng/mL, Ca 2.57+0.37 mmol/L, P 2.13+0.42 mmol/L, Mg 1.04+0.13 mmol/L, Ca2þ 1.29+0.04 mmol/L. Significant correlations were observed between the total ALP and Ca (r ¼ 0.5939; P ¼ 0.05) and OC and Ca (r ¼ 0.5706; P ¼ 0.05). Reference to the serum values of bone turnover parameters in sheep could be of great value in research and could provide complementary non-invasive information on the bone healing process, particularly with regard to obtaining an early prognosis of fracture healing. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01T00:00:00Z 2008 2011-12-28T10:17:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10348/1467 |
url |
http://hdl.handle.net/10348/1467 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137154064973824 |