Effect of hyperbaric storage on blood preservation

• Autor(a) principal: Martins, Ana Patrícia Lopes
• Data de Publicação: 2019
• Tipo de documento: Dissertação
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10773/27814
• topic: Hyperbaric Storage; Blood; Haemolysis; Preservation

This work aimed to evaluate the effect of a new preservation methodology, known as hyperbaric storage (HS) in combination with different temperatures as a possible alternative to the conventional method of storage (refrigeration) of whole blood. Two different solutions were added to the blood, an anticoagulant (Ethylenediaminetetraacetic Acid - EDTA) or an additive solution (Citrate Phosphate Dextrose Adenine - CPDA-1) and the samples were stored at atmospheric pressure at RT (0.1 MPa/RT), refrigerated temperatures (0.1 MPa/RF), 10 °C (0.1 MPa/10 °C) and under pressure (50 MPa/RT, 50 MPa/10 °C, 25 MPa/RT, 25 MPa/10 °C) up to 35 days. Samples with additive solution were also stored at 25 MPa/-5 °C for a period of 7 days. All the samples were analysed for microbial growth, haemolysis and pH variation during the storage period. The solution added to the blood showed to have impact in the haemolysis and microbial growth regardless the pressure-temperature combination during storage. The haemolysis was higher for the samples with only anticoagulant in the composition indicating the necessity of an additive solution in the blood. The samples stored under pressure revealed an increased haemolysis in the higher pressures (50 MPa/RT, 50 MPa/10 °C), but an inactivation effect regarding the microbial growth was verified. Lower temperatures had a positive impact in the blood when compared with the blood under the same pressure but at RT. At 25 MPa/-5 °C the lack of pH variation might open the possibility to storage blood without the production of lactic acid resulting from glycolysis. All the conditions were compared with the standard method of storage (refrigeration) and in none of the assays it was possible to increase the preservation time and keep a low haemolysis. Nevertheless, this thesis is the first work regarding the use of HS to preserve blood and more studies with other combinations of pressure-temperature must be tested.