Production of fish protein hydrolysates by a marine proteolytic strain

Detalhes bibliográficos
Autor(a) principal: Godinho, Irina Sofia Marques
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/6475
Resumo: Mestrado em Engenharia Alimentar - Instituto Superior de Agronomia
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spelling Production of fish protein hydrolysates by a marine proteolytic strainfermentative protein hydrolysateproteolytic strainfish by-productsantiradical activitychelating activityreducing powerMestrado em Engenharia Alimentar - Instituto Superior de AgronomiaThe preparation of fish protein hydrolysates by a fermentative process is an alternative to classical preparation using added proteolytic enzymes, taking advantage of the wide spectrum of proteolytic enzymes produced by microorganisms and increasing the complexity of the peptide mixture obtained. Thus, the objective of this work was to prepare fermentative protein hydrolysates (FPH) from a commercial cod protein hydrolysate (CPH) using an indigenous marine proteolytic strain. The bacterial growth was performed in liquid culture media, containing three concentrations of CPH (1, 2 and 4%), yeast extract (0.5%) and sucrose (0.5%), with continuous shaking, at 30 ºC for 24, 50 and 72 hours. The parameters evaluated were the culture growth, enzymatic activity, protein content and recovery, peptide profile, degree of hydrolysis and antioxidant activity, measured by DPPH and ABTS radical scavenging activity, reducing power and metal chelating activity. Bacterial growth and enzymatic activity reached their maximums after 24 hours of fermentation, and all FPH showed the disappearance of peptides in the range of 200 – 400 Da. In the set of samples, 4 % FPH recorded the highest values of protein content and recovery, but 1 % and 2 % FPH were the samples that presented improved antioxidant activity.ISAPedro, Sónia Cristina CorreiaMartins, Maria Luísa LouroRepositório da Universidade de LisboaGodinho, Irina Sofia Marques2014-02-07T10:45:43Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.5/6475TID:201920999porGodinho, I.S.M. - Production of fish protein hydrolysates by a marine proteolytic strain. Lisboa: ISA, 2013, 87 p.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:37:19Zoai:www.repository.utl.pt:10400.5/6475Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:53:51.003723Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Production of fish protein hydrolysates by a marine proteolytic strain
title Production of fish protein hydrolysates by a marine proteolytic strain
spellingShingle Production of fish protein hydrolysates by a marine proteolytic strain
Godinho, Irina Sofia Marques
fermentative protein hydrolysate
proteolytic strain
fish by-products
antiradical activity
chelating activity
reducing power
title_short Production of fish protein hydrolysates by a marine proteolytic strain
title_full Production of fish protein hydrolysates by a marine proteolytic strain
title_fullStr Production of fish protein hydrolysates by a marine proteolytic strain
title_full_unstemmed Production of fish protein hydrolysates by a marine proteolytic strain
title_sort Production of fish protein hydrolysates by a marine proteolytic strain
author Godinho, Irina Sofia Marques
author_facet Godinho, Irina Sofia Marques
author_role author
dc.contributor.none.fl_str_mv Pedro, Sónia Cristina Correia
Martins, Maria Luísa Louro
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Godinho, Irina Sofia Marques
dc.subject.por.fl_str_mv fermentative protein hydrolysate
proteolytic strain
fish by-products
antiradical activity
chelating activity
reducing power
topic fermentative protein hydrolysate
proteolytic strain
fish by-products
antiradical activity
chelating activity
reducing power
description Mestrado em Engenharia Alimentar - Instituto Superior de Agronomia
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
2014-02-07T10:45:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/6475
TID:201920999
url http://hdl.handle.net/10400.5/6475
identifier_str_mv TID:201920999
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Godinho, I.S.M. - Production of fish protein hydrolysates by a marine proteolytic strain. Lisboa: ISA, 2013, 87 p.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ISA
publisher.none.fl_str_mv ISA
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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