Mating type gene analyses in the genus Diplodia: from cryptic sex to cryptic species

Detalhes bibliográficos
Autor(a) principal: Lopes, Anabela
Data de Publicação: 2018
Outros Autores: Linaldeddu, Benedetto T., Phillips, Alan J. L., Alves, Artur
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/37640
Resumo: Cryptic species are common in Diplodia, a genus that includes some well-known and economically important plant pathogens. Thus, species delimitation has been based on the phylogenetic species recognition approach using multigene genealogies. We assessed the potential of mating type (MAT) genes sequences as phylogenetic markers for species delimitation in the genus Diplodia. A PCR-based mating type diagnostic assay was developed that allowed amplification and sequencing of the MAT1-1-1 and MAT1-2-1 genes, and determination of the mating strategies used by different species. All species tested were shown to be heterothallic. Phylogenetic analyses were performed on both MAT genes and also, for comparative purposes, on concatenated sequences of the ribosomal internal transcribed spacer (ITS), translation elongation factor 1-alpha (tef1-α) and beta-tubulin (tub2). Individual phylogenies based on MAT genes clearly differentiated all species analysed and agree with the results obtained with the commonly used multilocus phylogenetic analysis approach. However, MAT genes genealogies were superior to multigene genealogies in resolving closely related cryptic species. The phylogenetic informativeness of each locus was evaluated revealing that MAT genes were the most informative loci followed by tef1-α. Hence, MAT genes can be successfully used to establish species boundaries in the genus Diplodia.
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spelling Mating type gene analyses in the genus Diplodia: from cryptic sex to cryptic speciesBotryosphaeriaceaeHeterothallismPCR-based assayPhylogenetic informativenessCryptic species are common in Diplodia, a genus that includes some well-known and economically important plant pathogens. Thus, species delimitation has been based on the phylogenetic species recognition approach using multigene genealogies. We assessed the potential of mating type (MAT) genes sequences as phylogenetic markers for species delimitation in the genus Diplodia. A PCR-based mating type diagnostic assay was developed that allowed amplification and sequencing of the MAT1-1-1 and MAT1-2-1 genes, and determination of the mating strategies used by different species. All species tested were shown to be heterothallic. Phylogenetic analyses were performed on both MAT genes and also, for comparative purposes, on concatenated sequences of the ribosomal internal transcribed spacer (ITS), translation elongation factor 1-alpha (tef1-α) and beta-tubulin (tub2). Individual phylogenies based on MAT genes clearly differentiated all species analysed and agree with the results obtained with the commonly used multilocus phylogenetic analysis approach. However, MAT genes genealogies were superior to multigene genealogies in resolving closely related cryptic species. The phylogenetic informativeness of each locus was evaluated revealing that MAT genes were the most informative loci followed by tef1-α. Hence, MAT genes can be successfully used to establish species boundaries in the genus Diplodia.Elsevier2023-05-10T14:33:51Z2018-07-01T00:00:00Z2018-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/37640eng1878-614610.1016/j.funbio.2018.03.012Lopes, AnabelaLinaldeddu, Benedetto T.Phillips, Alan J. L.Alves, Arturinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:12:49Zoai:ria.ua.pt:10773/37640Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:08:14.636827Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Mating type gene analyses in the genus Diplodia: from cryptic sex to cryptic species
title Mating type gene analyses in the genus Diplodia: from cryptic sex to cryptic species
spellingShingle Mating type gene analyses in the genus Diplodia: from cryptic sex to cryptic species
Lopes, Anabela
Botryosphaeriaceae
Heterothallism
PCR-based assay
Phylogenetic informativeness
title_short Mating type gene analyses in the genus Diplodia: from cryptic sex to cryptic species
title_full Mating type gene analyses in the genus Diplodia: from cryptic sex to cryptic species
title_fullStr Mating type gene analyses in the genus Diplodia: from cryptic sex to cryptic species
title_full_unstemmed Mating type gene analyses in the genus Diplodia: from cryptic sex to cryptic species
title_sort Mating type gene analyses in the genus Diplodia: from cryptic sex to cryptic species
author Lopes, Anabela
author_facet Lopes, Anabela
Linaldeddu, Benedetto T.
Phillips, Alan J. L.
Alves, Artur
author_role author
author2 Linaldeddu, Benedetto T.
Phillips, Alan J. L.
Alves, Artur
author2_role author
author
author
dc.contributor.author.fl_str_mv Lopes, Anabela
Linaldeddu, Benedetto T.
Phillips, Alan J. L.
Alves, Artur
dc.subject.por.fl_str_mv Botryosphaeriaceae
Heterothallism
PCR-based assay
Phylogenetic informativeness
topic Botryosphaeriaceae
Heterothallism
PCR-based assay
Phylogenetic informativeness
description Cryptic species are common in Diplodia, a genus that includes some well-known and economically important plant pathogens. Thus, species delimitation has been based on the phylogenetic species recognition approach using multigene genealogies. We assessed the potential of mating type (MAT) genes sequences as phylogenetic markers for species delimitation in the genus Diplodia. A PCR-based mating type diagnostic assay was developed that allowed amplification and sequencing of the MAT1-1-1 and MAT1-2-1 genes, and determination of the mating strategies used by different species. All species tested were shown to be heterothallic. Phylogenetic analyses were performed on both MAT genes and also, for comparative purposes, on concatenated sequences of the ribosomal internal transcribed spacer (ITS), translation elongation factor 1-alpha (tef1-α) and beta-tubulin (tub2). Individual phylogenies based on MAT genes clearly differentiated all species analysed and agree with the results obtained with the commonly used multilocus phylogenetic analysis approach. However, MAT genes genealogies were superior to multigene genealogies in resolving closely related cryptic species. The phylogenetic informativeness of each locus was evaluated revealing that MAT genes were the most informative loci followed by tef1-α. Hence, MAT genes can be successfully used to establish species boundaries in the genus Diplodia.
publishDate 2018
dc.date.none.fl_str_mv 2018-07-01T00:00:00Z
2018-07
2023-05-10T14:33:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/37640
url http://hdl.handle.net/10773/37640
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1878-6146
10.1016/j.funbio.2018.03.012
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
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