Deacidification of endolysosomes by neuronal aging drives synapse loss

• Autor(a) principal: Burrinha, Tatiana
• Data de Publicação: 2023
• Outros Autores: Cunha, César, Hall, Michael J., Lopes-da-Silva, Mafalda, Seabra, Miguel C., Guimas Almeida, Cláudia
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10362/155155
• topic: aging; endolysosomal system; lysosome; neuron; synapses; Structural Biology; Biochemistry; Molecular Biology; Genetics; Cell Biology

Funding Information: The authors thank the lab members for technical assistance, helpful discussions and for the critical reading of the manuscript. The authors thank Dr. S. Miserey‐Lenkei (Institute Curie), Dr. D. Barral, Dr. O. Vieira and Dr. S. Tenreiro (NOVA Medical School), for the gift of antibodies and reagents. The authors thank Dr. A. Marques, Dr. C. Escrevente, Dr. C. Perdigão and Dr. L. Lopes for helpful discussions. The authors thank L. Almeida for excel macros. The authors thank Dr. D. Barral (NOVA Medical School) and Dr. O. Vieira (NOVA Medical School) for the critical reading of the manuscript. The authors thank Dr. A. Farinho (NOVA Medical School Histology Facility), Dr. S. Marques (NOVA Medical School Animal Facility), Dr. T. Pereira (NOVA Medical School Microscopy Platform) and the Electron Microscopy Facility (Instituto Gulbenkian de Ciência). This project has received funding from the Marie Curie Integration Grant (PCIG‐GA‐2012‐334366‐trafficinAD; Marie Curie Actions, EC); iNOVA4Health—UID/Multi/04462/2019, a program financially supported by Fundação para a Ciência e Tecnologia (FCT)/Ministério da Educação e Ciência, through national funds and cofunded by FEDER under the PT2020 Partnership Agreement; Maratona da Saúde 2016; Investigator FCT (IF/00998/2012, FCT); CEECIND/00410/2017 financed by FCT; ALZ AARG‐19‐618007 Alzheimer's Association; from the research infrastructure PPBI‐POCI‐01‐0145‐FEDER‐022122, co‐financed by FCT (Portugal) and Lisboa2020, under the PORTUGAL2020 agreement (European Regional Development Fund); the European Union's Horizon 2020 Research and Innovation Program under Grant agreement no. 811087 (Lysocil). TB has been the recipient of an FCT doctoral fellowship (SFRH/BD/131513/2017). MLS was funded by FCT‐CEECIND/01536/2018. Publisher Copyright: © 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.