Achieving Enzyme Stability Using a Simple Fabrication Procedure: The Alcohol Dehydrogenase Example
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.17265/2328-2150/2018.02 |
Resumo: | The use of screen-printing biosensors has been updated in this article as a tool to analyze the electron transfer process involving redox proteins or enzymes. The aim of this research was to fabricate a simple apparatus which allowed the use of the enzymes (in the solid state) to maintain their stability. To prove this concept an enzyme in the solid state was mixed with the carbon ink and this mixture was used to print the working electrode. We choose as proving the alcohol dehydrogenase. The first reason is because it metabolizes the alcohol, which can be present in biological samples of blood, saliva and urine and also in the beverage; the second is that this enzyme is still a challenge to electrochemistry due to having lower stability in sensors. The results show that in this device the enzyme was active and stable during all the experiments and in the experimental conditions that could catalyze the ethanol to acetaldehyde. These devices have the advantage of being disposable, cheap and are easy to fabricate. And also, they do not need expensive tools to be fabricated, they only need 2 μL of electrolyte or sample, and they need lower amounts of enzyme to permit electrochemical studies. |
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Achieving Enzyme Stability Using a Simple Fabrication Procedure: The Alcohol Dehydrogenase ExamplePaper biosensorDisposableAlcohol dehydrogenaseMultiple useThe use of screen-printing biosensors has been updated in this article as a tool to analyze the electron transfer process involving redox proteins or enzymes. The aim of this research was to fabricate a simple apparatus which allowed the use of the enzymes (in the solid state) to maintain their stability. To prove this concept an enzyme in the solid state was mixed with the carbon ink and this mixture was used to print the working electrode. We choose as proving the alcohol dehydrogenase. The first reason is because it metabolizes the alcohol, which can be present in biological samples of blood, saliva and urine and also in the beverage; the second is that this enzyme is still a challenge to electrochemistry due to having lower stability in sensors. The results show that in this device the enzyme was active and stable during all the experiments and in the experimental conditions that could catalyze the ethanol to acetaldehyde. These devices have the advantage of being disposable, cheap and are easy to fabricate. And also, they do not need expensive tools to be fabricated, they only need 2 μL of electrolyte or sample, and they need lower amounts of enzyme to permit electrochemical studies.DCM - Departamento de Ciência dos MateriaisRUNRibau, I.Fortunato, E.2019-05-02T22:19:00Z2018-02-282018-02-28T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.17265/2328-2150/2018.02eng2328-2150PURE: 12744409http://www.davidpublisher.org/index.php/Home/Article/index?id=34499.htmlhttps://doi.org/10.17265/2328-2150/2018.02info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:32:20Zoai:run.unl.pt:10362/68441Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:34:46.395316Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Achieving Enzyme Stability Using a Simple Fabrication Procedure: The Alcohol Dehydrogenase Example |
title |
Achieving Enzyme Stability Using a Simple Fabrication Procedure: The Alcohol Dehydrogenase Example |
spellingShingle |
Achieving Enzyme Stability Using a Simple Fabrication Procedure: The Alcohol Dehydrogenase Example Ribau, I. Paper biosensor Disposable Alcohol dehydrogenase Multiple use |
title_short |
Achieving Enzyme Stability Using a Simple Fabrication Procedure: The Alcohol Dehydrogenase Example |
title_full |
Achieving Enzyme Stability Using a Simple Fabrication Procedure: The Alcohol Dehydrogenase Example |
title_fullStr |
Achieving Enzyme Stability Using a Simple Fabrication Procedure: The Alcohol Dehydrogenase Example |
title_full_unstemmed |
Achieving Enzyme Stability Using a Simple Fabrication Procedure: The Alcohol Dehydrogenase Example |
title_sort |
Achieving Enzyme Stability Using a Simple Fabrication Procedure: The Alcohol Dehydrogenase Example |
author |
Ribau, I. |
author_facet |
Ribau, I. Fortunato, E. |
author_role |
author |
author2 |
Fortunato, E. |
author2_role |
author |
dc.contributor.none.fl_str_mv |
DCM - Departamento de Ciência dos Materiais RUN |
dc.contributor.author.fl_str_mv |
Ribau, I. Fortunato, E. |
dc.subject.por.fl_str_mv |
Paper biosensor Disposable Alcohol dehydrogenase Multiple use |
topic |
Paper biosensor Disposable Alcohol dehydrogenase Multiple use |
description |
The use of screen-printing biosensors has been updated in this article as a tool to analyze the electron transfer process involving redox proteins or enzymes. The aim of this research was to fabricate a simple apparatus which allowed the use of the enzymes (in the solid state) to maintain their stability. To prove this concept an enzyme in the solid state was mixed with the carbon ink and this mixture was used to print the working electrode. We choose as proving the alcohol dehydrogenase. The first reason is because it metabolizes the alcohol, which can be present in biological samples of blood, saliva and urine and also in the beverage; the second is that this enzyme is still a challenge to electrochemistry due to having lower stability in sensors. The results show that in this device the enzyme was active and stable during all the experiments and in the experimental conditions that could catalyze the ethanol to acetaldehyde. These devices have the advantage of being disposable, cheap and are easy to fabricate. And also, they do not need expensive tools to be fabricated, they only need 2 μL of electrolyte or sample, and they need lower amounts of enzyme to permit electrochemical studies. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-02-28 2018-02-28T00:00:00Z 2019-05-02T22:19:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.17265/2328-2150/2018.02 |
url |
https://doi.org/10.17265/2328-2150/2018.02 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2328-2150 PURE: 12744409 http://www.davidpublisher.org/index.php/Home/Article/index?id=34499.html https://doi.org/10.17265/2328-2150/2018.02 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137969847664640 |