Environmentally friendly bleaching of cotton using laccases

Detalhes bibliográficos
Autor(a) principal: Pereira, Luciana
Data de Publicação: 2005
Outros Autores: Bastos, C., Tzanov, Tzanko, Paulo, Artur Cavaco, Gübitz, Georg M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/12733
Resumo: A new strain of Trametes hirsuta was found to oxidize various cotton flavonoids. Here we show that laccases of this organism were responsible for oxidation of the flavonoids morin, luteolin, rutin and quercetin. Out of two laccases produced by T. hirsuta (60.7 and 51.0 kDa) the more prominent 60.7 kDa laccase was purified and showed Km and kcat values of 75.5, 20.9 and 49.4 μM and 72.5, 96.3 and 32.7 s−1, hours on ABTS, syringaldazide and DMP, respectively. Pretreatment of cotton with the T. hirsuta laccase resulted in a whiteness increase of 8.5%.
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spelling Environmentally friendly bleaching of cotton using laccasesBleaching of cottonTrametes hirsutalaccaseScience & TechnologyA new strain of Trametes hirsuta was found to oxidize various cotton flavonoids. Here we show that laccases of this organism were responsible for oxidation of the flavonoids morin, luteolin, rutin and quercetin. Out of two laccases produced by T. hirsuta (60.7 and 51.0 kDa) the more prominent 60.7 kDa laccase was purified and showed Km and kcat values of 75.5, 20.9 and 49.4 μM and 72.5, 96.3 and 32.7 s−1, hours on ABTS, syringaldazide and DMP, respectively. Pretreatment of cotton with the T. hirsuta laccase resulted in a whiteness increase of 8.5%.SpringerUniversidade do MinhoPereira, LucianaBastos, C.Tzanov, TzankoPaulo, Artur CavacoGübitz, Georg M.2005-112005-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/12733eng1610-365310.1007/s10311-005-0004-3http://www.springerlink.com/content/1610-3653/3/2/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:07:41Zoai:repositorium.sdum.uminho.pt:1822/12733Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:58:42.950193Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Environmentally friendly bleaching of cotton using laccases
title Environmentally friendly bleaching of cotton using laccases
spellingShingle Environmentally friendly bleaching of cotton using laccases
Pereira, Luciana
Bleaching of cotton
Trametes hirsuta
laccase
Science & Technology
title_short Environmentally friendly bleaching of cotton using laccases
title_full Environmentally friendly bleaching of cotton using laccases
title_fullStr Environmentally friendly bleaching of cotton using laccases
title_full_unstemmed Environmentally friendly bleaching of cotton using laccases
title_sort Environmentally friendly bleaching of cotton using laccases
author Pereira, Luciana
author_facet Pereira, Luciana
Bastos, C.
Tzanov, Tzanko
Paulo, Artur Cavaco
Gübitz, Georg M.
author_role author
author2 Bastos, C.
Tzanov, Tzanko
Paulo, Artur Cavaco
Gübitz, Georg M.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Pereira, Luciana
Bastos, C.
Tzanov, Tzanko
Paulo, Artur Cavaco
Gübitz, Georg M.
dc.subject.por.fl_str_mv Bleaching of cotton
Trametes hirsuta
laccase
Science & Technology
topic Bleaching of cotton
Trametes hirsuta
laccase
Science & Technology
description A new strain of Trametes hirsuta was found to oxidize various cotton flavonoids. Here we show that laccases of this organism were responsible for oxidation of the flavonoids morin, luteolin, rutin and quercetin. Out of two laccases produced by T. hirsuta (60.7 and 51.0 kDa) the more prominent 60.7 kDa laccase was purified and showed Km and kcat values of 75.5, 20.9 and 49.4 μM and 72.5, 96.3 and 32.7 s−1, hours on ABTS, syringaldazide and DMP, respectively. Pretreatment of cotton with the T. hirsuta laccase resulted in a whiteness increase of 8.5%.
publishDate 2005
dc.date.none.fl_str_mv 2005-11
2005-11-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/12733
url http://hdl.handle.net/1822/12733
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1610-3653
10.1007/s10311-005-0004-3
http://www.springerlink.com/content/1610-3653/3/2/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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