The ovine hepatic mitochondrial proteome: understanding seasonal weight loss tolerance in two distinct breeds
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.5/17538 |
Resumo: | Research Article |
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The ovine hepatic mitochondrial proteome: understanding seasonal weight loss tolerance in two distinct breedsovinebreedhepatic mitochondrial proteomeweight lossResearch ArticleSeasonal weight loss (SWL) is a primary constraint for farmers in the Mediterranean and tropics. One cost-effective solution to SWL is utilizing breeds like the Damara sheep that have adapted to deal with nutritional stress. Previous studies concluded that one of the adaptation mechanisms of SWL is a specialized fatty acid metabolism. Accordingly, hepatic-mitochondrial proteomes were compared across two different breeds (24 sheep total, Merino, n = 12 and Damara, n = 12) and two different diets (restricted vs unrestricted diet, 6 per breed, per diet, 24 total). Mitochondrial-proteins were isolated and relatively quantified using Blue native PAGE / 2D-electrophoresis and then analyzed via mass spectrometry. The tool ReviGO summarized the proteomes’ gene-ontology terms. A total of 50 proteins were identified with 7 changing significantly in abundance (ANOVA p-value<0.05). Specific abundance patterns of corticosteroid and inflammatory response-associated proteins such as annexin and glutamate dehydrogenase suggests that the Damara has an unusual inflammation response when subjected to SWL in addition to its unique metabolism. All significant proteins warrant further study; Annexin in particular shows promise as a potentially useful biomarkerPlos OneRepositório da Universidade de LisboaMiller, Blake A.Chapwanya, AspinasKilminster, TanyaScanion, TimMilton, JohnOsório, HugoOldham, ChrisGreeft, JohanBergfelt, Don R.Campos, Alexandre M.O.Almeida, André M.2019-03-08T09:46:10Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/17538engMiller BA, Chapwanya A, Kilminster T, Scanlon T, Milton J, Oso´rio H, et al. (2019) The ovine hepatic mitochondrial proteome: Understanding seasonal weight loss tolerance in two distinct breeds. PLoS ONE 14(2): e0212580https://doi.org/10.1371/journal.pone.0212580info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:47:13Zoai:www.repository.utl.pt:10400.5/17538Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:02:43.802002Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
The ovine hepatic mitochondrial proteome: understanding seasonal weight loss tolerance in two distinct breeds |
| title |
The ovine hepatic mitochondrial proteome: understanding seasonal weight loss tolerance in two distinct breeds |
| spellingShingle |
The ovine hepatic mitochondrial proteome: understanding seasonal weight loss tolerance in two distinct breeds Miller, Blake A. ovine breed hepatic mitochondrial proteome weight loss |
| title_short |
The ovine hepatic mitochondrial proteome: understanding seasonal weight loss tolerance in two distinct breeds |
| title_full |
The ovine hepatic mitochondrial proteome: understanding seasonal weight loss tolerance in two distinct breeds |
| title_fullStr |
The ovine hepatic mitochondrial proteome: understanding seasonal weight loss tolerance in two distinct breeds |
| title_full_unstemmed |
The ovine hepatic mitochondrial proteome: understanding seasonal weight loss tolerance in two distinct breeds |
| title_sort |
The ovine hepatic mitochondrial proteome: understanding seasonal weight loss tolerance in two distinct breeds |
| author |
Miller, Blake A. |
| author_facet |
Miller, Blake A. Chapwanya, Aspinas Kilminster, Tanya Scanion, Tim Milton, John Osório, Hugo Oldham, Chris Greeft, Johan Bergfelt, Don R. Campos, Alexandre M.O. Almeida, André M. |
| author_role |
author |
| author2 |
Chapwanya, Aspinas Kilminster, Tanya Scanion, Tim Milton, John Osório, Hugo Oldham, Chris Greeft, Johan Bergfelt, Don R. Campos, Alexandre M.O. Almeida, André M. |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Miller, Blake A. Chapwanya, Aspinas Kilminster, Tanya Scanion, Tim Milton, John Osório, Hugo Oldham, Chris Greeft, Johan Bergfelt, Don R. Campos, Alexandre M.O. Almeida, André M. |
| dc.subject.por.fl_str_mv |
ovine breed hepatic mitochondrial proteome weight loss |
| topic |
ovine breed hepatic mitochondrial proteome weight loss |
| description |
Research Article |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019-03-08T09:46:10Z 2019 2019-01-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/17538 |
| url |
http://hdl.handle.net/10400.5/17538 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Miller BA, Chapwanya A, Kilminster T, Scanlon T, Milton J, Oso´rio H, et al. (2019) The ovine hepatic mitochondrial proteome: Understanding seasonal weight loss tolerance in two distinct breeds. PLoS ONE 14(2): e0212580 https://doi.org/10.1371/journal.pone.0212580 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Plos One |
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Plos One |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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