Genetic analysis of Schistosoma mansoni sensitive and resistant strains to praziquantel using rapd-pcr

Detalhes bibliográficos
Autor(a) principal: Mendes, Tiago
Data de Publicação: 2018
Outros Autores: Calado, Manuela, Clemente, Isabel, Belo, Silvana, Afonso, Ana
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.25761/anaisihmt.189
Resumo: Praziquantel (PZQ) is the drug of choice in the treatment of schistosomiasis due to its high cure rates and no significant side effects. Currently, more and more cases of resistance or increased tolerance to PZQ have been reported, consequently, there were recommendations for continuous monitoring of drug resistance. In our laboratory, we selected a parasitic line of Schistosoma mansoni, through constant drug pressure over several cycles, which was resistant to 120 mg/kg PZQ. Adult parasites of S. mansoni from the resistant and from a sensible strain were collected; the DNA was extracted and analyzed through Random Amplified Polymorphic DNAPolymerase Chain Reaction (RAPD-PCR). We observed genetic polymorphism in 7 of 10 primers tested. Once observed the polymorphic differences between susceptible and resistant parasites, we calculated the similarity coefficient (Dice’s coefficient). Understanding the genetic polymorphism associated with resistance may contribute to the future identification of related sequences with the resistance/tolerance of S. mansoni to PZQ treatment and for identification of resistant parasites in the field as well as for the development of new strategies to schistosomiasis control.
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spelling Genetic analysis of Schistosoma mansoni sensitive and resistant strains to praziquantel using rapd-pcrAnálise genética de estirpes de Schistosoma mansoni sensíveis e resistentes a praziquantel por rapd-pcrPraziquantel (PZQ) is the drug of choice in the treatment of schistosomiasis due to its high cure rates and no significant side effects. Currently, more and more cases of resistance or increased tolerance to PZQ have been reported, consequently, there were recommendations for continuous monitoring of drug resistance. In our laboratory, we selected a parasitic line of Schistosoma mansoni, through constant drug pressure over several cycles, which was resistant to 120 mg/kg PZQ. Adult parasites of S. mansoni from the resistant and from a sensible strain were collected; the DNA was extracted and analyzed through Random Amplified Polymorphic DNAPolymerase Chain Reaction (RAPD-PCR). We observed genetic polymorphism in 7 of 10 primers tested. Once observed the polymorphic differences between susceptible and resistant parasites, we calculated the similarity coefficient (Dice’s coefficient). Understanding the genetic polymorphism associated with resistance may contribute to the future identification of related sequences with the resistance/tolerance of S. mansoni to PZQ treatment and for identification of resistant parasites in the field as well as for the development of new strategies to schistosomiasis control.O praziquantel (PZQ) é o fármaco de eleição no tratamento da schistosomose, devido à sua alta taxa de cura e por não demostrar efeitos secundários significativos mas recentemente têm sido reportados cada vez mais casos de resistência ou de aumento de tolerância a este fármaco. Devido a estas observações, a OMS recomenda a monitorização contínua de Schistosoma spp potencialmente resistentes ao fármaco. No laboratório de Helmintologia foi seleccionada uma linha de Schistosoma mansoni, resistente a PZQ na dose de 120 mg/Kg, após pressão de fármaco constante ao longo de vários ciclos. Foram recolhidos parasitas adultos de S. mansoni da estirpe resistente e de uma estirpe sensível e procedeu-se à análise do DNA dos vermes por Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR). Foi possível observar polimorfismos em 7 dos 10 primers. Uma vez observadas as diferenças polimórficas entre parasitas sensíveis e resistentes, calculou-se o coeficiente de similaridade (Dice’s coefficient). A compreensão de polimorfismos genéticos associados à resistência poderão contribuir para a identificação futura de sequências relacionadas com a resistência/tolerância de S. mansoni ao tratamento por PZQ e para a identificação de parasitas resistentes no campo, assim como para o desenvolvimento de novas estratégias de controlo para a schistosomose.Universidade Nova de Lisboa2018-09-08T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.25761/anaisihmt.189oai:ojs.anaisihmt.com:article/189Anais do Instituto de Higiene e Medicina Tropical; Vol 12 (2013): 2º Congresso Nacional de Medicina Tropical; 41-45Anais do Instituto de Higiene e Medicina Tropical; v. 12 (2013): 2º Congresso Nacional de Medicina Tropical; 41-452184-23100303-7762reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPporhttp://anaisihmt.com/index.php/ihmt/article/view/189https://doi.org/10.25761/anaisihmt.189http://anaisihmt.com/index.php/ihmt/article/view/189/153Mendes, TiagoCalado, ManuelaClemente, IsabelBelo, SilvanaAfonso, Anainfo:eu-repo/semantics/openAccess2022-09-23T15:30:22Zoai:ojs.anaisihmt.com:article/189Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:03:56.449544Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genetic analysis of Schistosoma mansoni sensitive and resistant strains to praziquantel using rapd-pcr
Análise genética de estirpes de Schistosoma mansoni sensíveis e resistentes a praziquantel por rapd-pcr
title Genetic analysis of Schistosoma mansoni sensitive and resistant strains to praziquantel using rapd-pcr
spellingShingle Genetic analysis of Schistosoma mansoni sensitive and resistant strains to praziquantel using rapd-pcr
Mendes, Tiago
title_short Genetic analysis of Schistosoma mansoni sensitive and resistant strains to praziquantel using rapd-pcr
title_full Genetic analysis of Schistosoma mansoni sensitive and resistant strains to praziquantel using rapd-pcr
title_fullStr Genetic analysis of Schistosoma mansoni sensitive and resistant strains to praziquantel using rapd-pcr
title_full_unstemmed Genetic analysis of Schistosoma mansoni sensitive and resistant strains to praziquantel using rapd-pcr
title_sort Genetic analysis of Schistosoma mansoni sensitive and resistant strains to praziquantel using rapd-pcr
author Mendes, Tiago
author_facet Mendes, Tiago
Calado, Manuela
Clemente, Isabel
Belo, Silvana
Afonso, Ana
author_role author
author2 Calado, Manuela
Clemente, Isabel
Belo, Silvana
Afonso, Ana
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Mendes, Tiago
Calado, Manuela
Clemente, Isabel
Belo, Silvana
Afonso, Ana
description Praziquantel (PZQ) is the drug of choice in the treatment of schistosomiasis due to its high cure rates and no significant side effects. Currently, more and more cases of resistance or increased tolerance to PZQ have been reported, consequently, there were recommendations for continuous monitoring of drug resistance. In our laboratory, we selected a parasitic line of Schistosoma mansoni, through constant drug pressure over several cycles, which was resistant to 120 mg/kg PZQ. Adult parasites of S. mansoni from the resistant and from a sensible strain were collected; the DNA was extracted and analyzed through Random Amplified Polymorphic DNAPolymerase Chain Reaction (RAPD-PCR). We observed genetic polymorphism in 7 of 10 primers tested. Once observed the polymorphic differences between susceptible and resistant parasites, we calculated the similarity coefficient (Dice’s coefficient). Understanding the genetic polymorphism associated with resistance may contribute to the future identification of related sequences with the resistance/tolerance of S. mansoni to PZQ treatment and for identification of resistant parasites in the field as well as for the development of new strategies to schistosomiasis control.
publishDate 2018
dc.date.none.fl_str_mv 2018-09-08T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.25761/anaisihmt.189
oai:ojs.anaisihmt.com:article/189
url https://doi.org/10.25761/anaisihmt.189
identifier_str_mv oai:ojs.anaisihmt.com:article/189
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://anaisihmt.com/index.php/ihmt/article/view/189
https://doi.org/10.25761/anaisihmt.189
http://anaisihmt.com/index.php/ihmt/article/view/189/153
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Nova de Lisboa
publisher.none.fl_str_mv Universidade Nova de Lisboa
dc.source.none.fl_str_mv Anais do Instituto de Higiene e Medicina Tropical; Vol 12 (2013): 2º Congresso Nacional de Medicina Tropical; 41-45
Anais do Instituto de Higiene e Medicina Tropical; v. 12 (2013): 2º Congresso Nacional de Medicina Tropical; 41-45
2184-2310
0303-7762
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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