Nanoencapsulation of Gla-Rich Protein (GRP) as a Novel Approach to Target Inflammation
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/143302 |
Resumo: | LA/P/0101/2020 LA/P/0140/2020 AAC nº 41/ALG/2020—Project nº 072583—NUTRISAFE |
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Nanoencapsulation of Gla-Rich Protein (GRP) as a Novel Approach to Target Inflammationchronic inflammatory diseases (CIDs)Gla-rich protein (GRP)inflammationnanoparticlesvitamin K-dependent protein (VKDP)CatalysisMolecular BiologySpectroscopyComputer Science ApplicationsPhysical and Theoretical ChemistryOrganic ChemistryInorganic ChemistrySDG 3 - Good Health and Well-beingLA/P/0101/2020 LA/P/0140/2020 AAC nº 41/ALG/2020—Project nº 072583—NUTRISAFEChronic inflammation is a major driver of chronic inflammatory diseases (CIDs), with a tremendous impact worldwide. Besides its function as a pathological calcification inhibitor, vitamin K-dependent protein Gla-rich protein (GRP) was shown to act as an anti-inflammatory agent independently of its gamma-carboxylation status. Although GRP’s therapeutic potential has been highlighted, its low solubility at physiological pH still constitutes a major challenge for its biomedical application. In this work, we produced fluorescein-labeled chitosan-tripolyphosphate nanoparticles containing non-carboxylated GRP (ucGRP) (FCNG) via ionotropic gelation, increasing its bioavail-ability, stability, and anti-inflammatory potential. The results indicate the nanosized nature of FCNG with PDI and a zeta potential suitable for biomedical applications. FCNG’s anti-inflammatory activity was studied in macrophage-differentiated THP1 cells, and in primary vascular smooth muscle cells and chondrocytes, inflamed with LPS, TNFα and IL-1β, respectively. In all these in vitro human cell systems, FCNG treatments resulted in increased intra and extracellular GRP levels, and decreased pro-inflammatory responses of target cells, by decreasing pro-inflammatory cytokines and inflammation mediators. These results suggest the retained anti-inflammatory bioactivity of ucGRP in FCNG, strengthening the potential use of ucGRP as an anti-inflammatory agent with a wide spectrum of application, and opening up perspectives for its therapeutic application in CIDs.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaInstituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNViegas, Carla S. B.Araújo, NunaCarreira, JoanaPontes, Jorge F.Macedo, Anjos L.Vinhas, MauríciaMoreira, Ana S.Faria, Tiago Q.Grenha, Anade Matos, António A.Schurgers, LeonVermeer, CeesSimes, Dina Costa2022-08-25T22:20:28Z2022-04-272022-04-27T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article18application/pdfhttp://hdl.handle.net/10362/143302eng1661-6596PURE: 43766516https://doi.org/10.3390/ijms23094813info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:21:36Zoai:run.unl.pt:10362/143302Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:50:49.197750Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Nanoencapsulation of Gla-Rich Protein (GRP) as a Novel Approach to Target Inflammation |
| title |
Nanoencapsulation of Gla-Rich Protein (GRP) as a Novel Approach to Target Inflammation |
| spellingShingle |
Nanoencapsulation of Gla-Rich Protein (GRP) as a Novel Approach to Target Inflammation Viegas, Carla S. B. chronic inflammatory diseases (CIDs) Gla-rich protein (GRP) inflammation nanoparticles vitamin K-dependent protein (VKDP) Catalysis Molecular Biology Spectroscopy Computer Science Applications Physical and Theoretical Chemistry Organic Chemistry Inorganic Chemistry SDG 3 - Good Health and Well-being |
| title_short |
Nanoencapsulation of Gla-Rich Protein (GRP) as a Novel Approach to Target Inflammation |
| title_full |
Nanoencapsulation of Gla-Rich Protein (GRP) as a Novel Approach to Target Inflammation |
| title_fullStr |
Nanoencapsulation of Gla-Rich Protein (GRP) as a Novel Approach to Target Inflammation |
| title_full_unstemmed |
Nanoencapsulation of Gla-Rich Protein (GRP) as a Novel Approach to Target Inflammation |
| title_sort |
Nanoencapsulation of Gla-Rich Protein (GRP) as a Novel Approach to Target Inflammation |
| author |
Viegas, Carla S. B. |
| author_facet |
Viegas, Carla S. B. Araújo, Nuna Carreira, Joana Pontes, Jorge F. Macedo, Anjos L. Vinhas, Maurícia Moreira, Ana S. Faria, Tiago Q. Grenha, Ana de Matos, António A. Schurgers, Leon Vermeer, Cees Simes, Dina Costa |
| author_role |
author |
| author2 |
Araújo, Nuna Carreira, Joana Pontes, Jorge F. Macedo, Anjos L. Vinhas, Maurícia Moreira, Ana S. Faria, Tiago Q. Grenha, Ana de Matos, António A. Schurgers, Leon Vermeer, Cees Simes, Dina Costa |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
UCIBIO - Applied Molecular Biosciences Unit DQ - Departamento de Química Instituto de Tecnologia Química e Biológica António Xavier (ITQB) RUN |
| dc.contributor.author.fl_str_mv |
Viegas, Carla S. B. Araújo, Nuna Carreira, Joana Pontes, Jorge F. Macedo, Anjos L. Vinhas, Maurícia Moreira, Ana S. Faria, Tiago Q. Grenha, Ana de Matos, António A. Schurgers, Leon Vermeer, Cees Simes, Dina Costa |
| dc.subject.por.fl_str_mv |
chronic inflammatory diseases (CIDs) Gla-rich protein (GRP) inflammation nanoparticles vitamin K-dependent protein (VKDP) Catalysis Molecular Biology Spectroscopy Computer Science Applications Physical and Theoretical Chemistry Organic Chemistry Inorganic Chemistry SDG 3 - Good Health and Well-being |
| topic |
chronic inflammatory diseases (CIDs) Gla-rich protein (GRP) inflammation nanoparticles vitamin K-dependent protein (VKDP) Catalysis Molecular Biology Spectroscopy Computer Science Applications Physical and Theoretical Chemistry Organic Chemistry Inorganic Chemistry SDG 3 - Good Health and Well-being |
| description |
LA/P/0101/2020 LA/P/0140/2020 AAC nº 41/ALG/2020—Project nº 072583—NUTRISAFE |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-08-25T22:20:28Z 2022-04-27 2022-04-27T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/143302 |
| url |
http://hdl.handle.net/10362/143302 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
1661-6596 PURE: 43766516 https://doi.org/10.3390/ijms23094813 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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18 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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