Smarcad1 mediates microbiota-induced inflammation in mouse and coordinates gene expression in the intestinal epithelium

Detalhes bibliográficos
Autor(a) principal: Kazakevych, Juri
Data de Publicação: 2020
Outros Autores: Denizot, Jérémy, Liebert, Anke, Portovedo, Mariana, Mosavie, Mia, Jain, Payal, Stellato, Claudia, Fraser, Claire, Corrêa, Renan Oliveira, Célestine, Marina, Mattiuz, Raphaël, Okkenhaug, Hanneke, Miller, J. Ross, Vinolo, Marco Aurélio Ramirez, Veldhoen, Marc, Varga-Weisz, Patrick
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/49900
Resumo: © The Author(s). 2020. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
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spelling Smarcad1 mediates microbiota-induced inflammation in mouse and coordinates gene expression in the intestinal epithelium© The Author(s). 2020. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.Background: How intestinal epithelial cells interact with the microbiota and how this is regulated at the gene expression level are critical questions. Smarcad1 is a conserved chromatin remodeling factor with a poorly understood tissue function. As this factor is highly expressed in the stem and proliferative zones of the intestinal epithelium, we explore its role in this tissue. Results: Specific deletion of Smarcad1 in the mouse intestinal epithelium leads to colitis resistance and substantial changes in gene expression, including a striking increase of expression of several genes linked to innate immunity. Absence of Smarcad1 leads to changes in chromatin accessibility and significant changes in histone H3K9me3 over many sites, including genes that are differentially regulated upon Smarcad1 deletion. We identify candidate members of the gut microbiome that elicit a Smarcad1-dependent colitis response, including members of the poorly understood TM7 phylum. Conclusions: Our study sheds light onto the role of the chromatin remodeling machinery in intestinal epithelial cells in the colitis response and shows how a highly conserved chromatin remodeling factor has a distinct role in anti-microbial defense. This work highlights the importance of the intestinal epithelium in the colitis response and the potential of microbial species as pharmacological and probiotic targets in the context of inflammatory diseases.This work was supported by BBSRC Institute Strategic Programme Grants (BBS/E/B/000C0404, BBS/E/B/000C0405), a MRC project grant to PVW and MV (MR/N009398/1), a BBSRC-Fundação de Amparo à Pesquisa do Estado de São Paulo/FAPESP-Brazil Pump-priming award (BB/N013565/1,FAPESP#2015/50379-1), and a Newton Advanced Fellowship from the Royal Society to MARV and PVW (NAF\R1\180116).Springer NatureRepositório da Universidade de LisboaKazakevych, JuriDenizot, JérémyLiebert, AnkePortovedo, MarianaMosavie, MiaJain, PayalStellato, ClaudiaFraser, ClaireCorrêa, Renan OliveiraCélestine, MarinaMattiuz, RaphaëlOkkenhaug, HannekeMiller, J. RossVinolo, Marco Aurélio RamirezVeldhoen, MarcVarga-Weisz, Patrick2021-10-15T13:15:11Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/49900engGenome Biol. 2020 Mar 11;21(1):641474-760X10.1186/s13059-020-01976-71465-6906info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:53:52Zoai:repositorio.ul.pt:10451/49900Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:01:25.590775Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Smarcad1 mediates microbiota-induced inflammation in mouse and coordinates gene expression in the intestinal epithelium
title Smarcad1 mediates microbiota-induced inflammation in mouse and coordinates gene expression in the intestinal epithelium
spellingShingle Smarcad1 mediates microbiota-induced inflammation in mouse and coordinates gene expression in the intestinal epithelium
Kazakevych, Juri
title_short Smarcad1 mediates microbiota-induced inflammation in mouse and coordinates gene expression in the intestinal epithelium
title_full Smarcad1 mediates microbiota-induced inflammation in mouse and coordinates gene expression in the intestinal epithelium
title_fullStr Smarcad1 mediates microbiota-induced inflammation in mouse and coordinates gene expression in the intestinal epithelium
title_full_unstemmed Smarcad1 mediates microbiota-induced inflammation in mouse and coordinates gene expression in the intestinal epithelium
title_sort Smarcad1 mediates microbiota-induced inflammation in mouse and coordinates gene expression in the intestinal epithelium
author Kazakevych, Juri
author_facet Kazakevych, Juri
Denizot, Jérémy
Liebert, Anke
Portovedo, Mariana
Mosavie, Mia
Jain, Payal
Stellato, Claudia
Fraser, Claire
Corrêa, Renan Oliveira
Célestine, Marina
Mattiuz, Raphaël
Okkenhaug, Hanneke
Miller, J. Ross
Vinolo, Marco Aurélio Ramirez
Veldhoen, Marc
Varga-Weisz, Patrick
author_role author
author2 Denizot, Jérémy
Liebert, Anke
Portovedo, Mariana
Mosavie, Mia
Jain, Payal
Stellato, Claudia
Fraser, Claire
Corrêa, Renan Oliveira
Célestine, Marina
Mattiuz, Raphaël
Okkenhaug, Hanneke
Miller, J. Ross
Vinolo, Marco Aurélio Ramirez
Veldhoen, Marc
Varga-Weisz, Patrick
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Kazakevych, Juri
Denizot, Jérémy
Liebert, Anke
Portovedo, Mariana
Mosavie, Mia
Jain, Payal
Stellato, Claudia
Fraser, Claire
Corrêa, Renan Oliveira
Célestine, Marina
Mattiuz, Raphaël
Okkenhaug, Hanneke
Miller, J. Ross
Vinolo, Marco Aurélio Ramirez
Veldhoen, Marc
Varga-Weisz, Patrick
description © The Author(s). 2020. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
2021-10-15T13:15:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/49900
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dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv Genome Biol. 2020 Mar 11;21(1):64
1474-760X
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