Data from the manuscript [A role of supraspinal galanin in behavioural hyperalgesia in the rat]
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Conjunto de dados |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/30587 |
Resumo: | Manuscript: "A role of supraspinal galanin in behavioural hyperalgesia in the rat" – by Amorim et al, PLoS One. Available at: http://hdl.handle.net/1822/32480 |
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Data from the manuscript [A role of supraspinal galanin in behavioural hyperalgesia in the rat]experimental monoarthritispronociception, galaningalanin receptor type-1dorsomedial hypothalamic nucleusrostral ventromedial medullaManuscript: "A role of supraspinal galanin in behavioural hyperalgesia in the rat" – by Amorim et al, PLoS One. Available at: http://hdl.handle.net/1822/32480In chronic pain disorders, galanin (GAL) is able to either facilitate or inhibit nociception in the spinal cord but the contribution of supraspinal galanin to pain signalling is mostly unknown. The dorsomedial nucleus of the hypothalamus (DMH) is rich in galanin receptors (GALR) and is involved in behavioural hyperalgesia. In this study, we evaluated the contribution of supraspinal GAL to behavioural hyperalgesia in experimental monoarthritis. In Wistar-Han males with a four week kaolin/carrageenan-induced monoarthritis (ARTH), paw-withdrawal latency (PWL) was assessed before and after DMH administration of exogenous GAL, a non-specific GALR antagonist (M40), a specific GALR1 agonist (M617) and a specific GALR2 antagonist (M871). Additionally, the analysis of c-Fos expression after GAL injection in the DMH was used to investigate the potential involvement of brainstem pain control centres. Finally, electrophysiological recordings were performed to evaluate whether pronociceptive On- or antinociceptive Off-like cells in the rostral ventromedial medulla (RVM) relay the effect of GAL. Exogenous GAL in the DMH decreased PWL in ARTH and SHAM animals, an effect that was mimicked by a GALR1 agonist (M617). In SHAM animals, an unselective GALR antagonist (M40) increased PWL, while a GALR2 antagonist (M871) decreased PWL. M40 or M871 failed to influence PWL in ARTH animals. Exogenous GAL increased c-Fos expression in the RVM and dorsal raphe nucleus (DRN), with effects being more prominent in SHAM than ARTH animals. Exogenous GAL failed to influence activity of RVM On- or Off-like cells of SHAM and ARTH animals. Overall, exogenous GAL in the DMH had a pronociceptive effect that is mediated by GALR1 in healthy and arthritic animals and is associated with alterations of c-Fos expression in RVM and DRN that are serotonergic brainstem nuclei known to be involved in the regulation of pain.This study was supported by grants from the Portuguese Science Foundation (FCT) Project nu PTDC/SAU-NEU/108557/2008, FEDER-COMPETE, by the Academy of Finland and the Sigrid Jusélius Foundation, Helsinki, Finland. DA was supported by FCT grant SFRH/BD/71219/2010 and ADP was supported by FCT grant SFRH/BD/90374/2012. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Universidade do MinhoAmorim, DianaDavid-Pereira, AnaMarques, PatríciaPuga, Sónia Andreia SilvaRebelo, PatríciaCosta, Patrício SoaresPertovaara, AnttiAlmeida, ArmandoPinto-Ribeiro, Filipa20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/datasetapplication/octet-streamhttp://hdl.handle.net/1822/30587enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:51:18Zoai:repositorium.sdum.uminho.pt:1822/30587Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:50:10.174884Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Data from the manuscript [A role of supraspinal galanin in behavioural hyperalgesia in the rat] |
title |
Data from the manuscript [A role of supraspinal galanin in behavioural hyperalgesia in the rat] |
spellingShingle |
Data from the manuscript [A role of supraspinal galanin in behavioural hyperalgesia in the rat] Amorim, Diana experimental monoarthritis pronociception, galanin galanin receptor type-1 dorsomedial hypothalamic nucleus rostral ventromedial medulla |
title_short |
Data from the manuscript [A role of supraspinal galanin in behavioural hyperalgesia in the rat] |
title_full |
Data from the manuscript [A role of supraspinal galanin in behavioural hyperalgesia in the rat] |
title_fullStr |
Data from the manuscript [A role of supraspinal galanin in behavioural hyperalgesia in the rat] |
title_full_unstemmed |
Data from the manuscript [A role of supraspinal galanin in behavioural hyperalgesia in the rat] |
title_sort |
Data from the manuscript [A role of supraspinal galanin in behavioural hyperalgesia in the rat] |
author |
Amorim, Diana |
author_facet |
Amorim, Diana David-Pereira, Ana Marques, Patrícia Puga, Sónia Andreia Silva Rebelo, Patrícia Costa, Patrício Soares Pertovaara, Antti Almeida, Armando Pinto-Ribeiro, Filipa |
author_role |
author |
author2 |
David-Pereira, Ana Marques, Patrícia Puga, Sónia Andreia Silva Rebelo, Patrícia Costa, Patrício Soares Pertovaara, Antti Almeida, Armando Pinto-Ribeiro, Filipa |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Amorim, Diana David-Pereira, Ana Marques, Patrícia Puga, Sónia Andreia Silva Rebelo, Patrícia Costa, Patrício Soares Pertovaara, Antti Almeida, Armando Pinto-Ribeiro, Filipa |
dc.subject.por.fl_str_mv |
experimental monoarthritis pronociception, galanin galanin receptor type-1 dorsomedial hypothalamic nucleus rostral ventromedial medulla |
topic |
experimental monoarthritis pronociception, galanin galanin receptor type-1 dorsomedial hypothalamic nucleus rostral ventromedial medulla |
description |
Manuscript: "A role of supraspinal galanin in behavioural hyperalgesia in the rat" – by Amorim et al, PLoS One. Available at: http://hdl.handle.net/1822/32480 |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/dataset |
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dataset |
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http://hdl.handle.net/1822/30587 |
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http://hdl.handle.net/1822/30587 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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