Modulating the unfolded protein response with ONC201 to impact on radiation response in prostate cancer cells

Detalhes bibliográficos
Autor(a) principal: Amoroso, Francesca
Data de Publicação: 2021
Outros Autores: Glass, Kimberley, Singh, Reema, Liberal, Francisco, Steele, Rebecca E., Maguire, Sarah, Tarapore, Rohinton, Allen, Joshua E., Van Schaeybroeck, Sandra, Butterworth, Karl T., Prise, Kevin, O’Sullivan, Joe M., Jain, Suneil, Waugh, David J., Mills, Ian G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/145942
Resumo: Funding Information: This work was supported by Movember Prostate Cancer UK Centre of Excellence (CEO13_2-004) to JOS and DJW and also by a grant from the Norwegian Research Council (230559) to IGM. IGM is also supported by the John Black Charitable Research Foundation. The authors acknowledge the Genomic Core Technology Unit, CCRCB, Queen’s University Belfast that performed the RNA-Seq Sequencing; the support of Fundação para a Ciência e Tecnologia (FCT-MCTES), Radiation Biology and Biophysics Doctoral Training Programme (RaBBiT, PD/00193/2012); and scholarships grants number SFRH/BD/114448/2016 to (FL). Publisher Copyright: © 2021, The Author(s).
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spelling Modulating the unfolded protein response with ONC201 to impact on radiation response in prostate cancer cellsGeneralSDG 3 - Good Health and Well-beingFunding Information: This work was supported by Movember Prostate Cancer UK Centre of Excellence (CEO13_2-004) to JOS and DJW and also by a grant from the Norwegian Research Council (230559) to IGM. IGM is also supported by the John Black Charitable Research Foundation. The authors acknowledge the Genomic Core Technology Unit, CCRCB, Queen’s University Belfast that performed the RNA-Seq Sequencing; the support of Fundação para a Ciência e Tecnologia (FCT-MCTES), Radiation Biology and Biophysics Doctoral Training Programme (RaBBiT, PD/00193/2012); and scholarships grants number SFRH/BD/114448/2016 to (FL). Publisher Copyright: © 2021, The Author(s).Prostate cancer (PCa) is the most common non-cutaneous cancer in men and a notable cause of cancer mortality when it metastasises. The unfolded protein response (UPR) can be cytoprotective but when acutely activated can lead to cell death. In this study, we sought to enhance the acute activation of the UPR using radiation and ONC201, an UPR activator. Treating PCa cells with ONC201 quickly increased the expression of all the key regulators of the UPR and reduced the oxidative phosphorylation, with cell death occurring 72 h later. We exploited this time lag to sensitize prostate cancer cells to radiation through short-term treatment with ONC201. To understand how priming occurred, we performed RNA-Seq analysis and found that ONC201 suppressed the expression of cell cycle and DNA repair factors. In conclusion, we have shown that ONC201 can prime enhanced radiation response.Faculdade de Ciências e Tecnologia (FCT)RUNAmoroso, FrancescaGlass, KimberleySingh, ReemaLiberal, FranciscoSteele, Rebecca E.Maguire, SarahTarapore, RohintonAllen, Joshua E.Van Schaeybroeck, SandraButterworth, Karl T.Prise, KevinO’Sullivan, Joe M.Jain, SuneilWaugh, David J.Mills, Ian G.2022-12-02T22:13:25Z2021-122021-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/145942eng2045-2322PURE: 45531474https://doi.org/10.1038/s41598-021-83215-yinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:26:45Zoai:run.unl.pt:10362/145942Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:52:21.354070Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Modulating the unfolded protein response with ONC201 to impact on radiation response in prostate cancer cells
title Modulating the unfolded protein response with ONC201 to impact on radiation response in prostate cancer cells
spellingShingle Modulating the unfolded protein response with ONC201 to impact on radiation response in prostate cancer cells
Amoroso, Francesca
General
SDG 3 - Good Health and Well-being
title_short Modulating the unfolded protein response with ONC201 to impact on radiation response in prostate cancer cells
title_full Modulating the unfolded protein response with ONC201 to impact on radiation response in prostate cancer cells
title_fullStr Modulating the unfolded protein response with ONC201 to impact on radiation response in prostate cancer cells
title_full_unstemmed Modulating the unfolded protein response with ONC201 to impact on radiation response in prostate cancer cells
title_sort Modulating the unfolded protein response with ONC201 to impact on radiation response in prostate cancer cells
author Amoroso, Francesca
author_facet Amoroso, Francesca
Glass, Kimberley
Singh, Reema
Liberal, Francisco
Steele, Rebecca E.
Maguire, Sarah
Tarapore, Rohinton
Allen, Joshua E.
Van Schaeybroeck, Sandra
Butterworth, Karl T.
Prise, Kevin
O’Sullivan, Joe M.
Jain, Suneil
Waugh, David J.
Mills, Ian G.
author_role author
author2 Glass, Kimberley
Singh, Reema
Liberal, Francisco
Steele, Rebecca E.
Maguire, Sarah
Tarapore, Rohinton
Allen, Joshua E.
Van Schaeybroeck, Sandra
Butterworth, Karl T.
Prise, Kevin
O’Sullivan, Joe M.
Jain, Suneil
Waugh, David J.
Mills, Ian G.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Faculdade de Ciências e Tecnologia (FCT)
RUN
dc.contributor.author.fl_str_mv Amoroso, Francesca
Glass, Kimberley
Singh, Reema
Liberal, Francisco
Steele, Rebecca E.
Maguire, Sarah
Tarapore, Rohinton
Allen, Joshua E.
Van Schaeybroeck, Sandra
Butterworth, Karl T.
Prise, Kevin
O’Sullivan, Joe M.
Jain, Suneil
Waugh, David J.
Mills, Ian G.
dc.subject.por.fl_str_mv General
SDG 3 - Good Health and Well-being
topic General
SDG 3 - Good Health and Well-being
description Funding Information: This work was supported by Movember Prostate Cancer UK Centre of Excellence (CEO13_2-004) to JOS and DJW and also by a grant from the Norwegian Research Council (230559) to IGM. IGM is also supported by the John Black Charitable Research Foundation. The authors acknowledge the Genomic Core Technology Unit, CCRCB, Queen’s University Belfast that performed the RNA-Seq Sequencing; the support of Fundação para a Ciência e Tecnologia (FCT-MCTES), Radiation Biology and Biophysics Doctoral Training Programme (RaBBiT, PD/00193/2012); and scholarships grants number SFRH/BD/114448/2016 to (FL). Publisher Copyright: © 2021, The Author(s).
publishDate 2021
dc.date.none.fl_str_mv 2021-12
2021-12-01T00:00:00Z
2022-12-02T22:13:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/145942
url http://hdl.handle.net/10362/145942
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2045-2322
PURE: 45531474
https://doi.org/10.1038/s41598-021-83215-y
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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