The 1.4 Å resolution structure of Paracoccus pantotrophus pseudoazurin
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.1107/S1744309110013989 |
Resumo: | This work was supported in part by Fundação para a Ciência e a Tecnologia (Lisbon, Portugal) through project grant POCTI/QUI/64638/2006 and individual grant SFRH/BPD/20357/2004 (SN). The authors would like to thank Dr Cecilia Bonifacio for help with crystallization and Dr Jose Trincao for help with data collection. |
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The 1.4 Å resolution structure of Paracoccus pantotrophus pseudoazurinCopper proteinsElectron transferParacoccus pantotrophusPseudoazurinType 1 blue copper protein familyBiophysicsStructural BiologyBiochemistryGeneticsCondensed Matter PhysicsThis work was supported in part by Fundação para a Ciência e a Tecnologia (Lisbon, Portugal) through project grant POCTI/QUI/64638/2006 and individual grant SFRH/BPD/20357/2004 (SN). The authors would like to thank Dr Cecilia Bonifacio for help with crystallization and Dr Jose Trincao for help with data collection.Pseudoazurins are small type 1 copper proteins that are involved in the flow of electrons between various electron donors and acceptors in the bacterial periplasm, mostly under denitrifying conditions. The previously determined structure of Paracoccus pantotrophus pseudoazurin in the oxidized form was improved to a nominal resolution of 1.4 Å, with R and R free values of 0.188 and 0.206, respectively. This high-resolution structure makes it possible to analyze the interactions between the monomers and the solvent structure in detail. Analysis of the high-resolution structure revealed the structural regions that are responsible for monomer-monomer recognition during dimer formation and for protein-protein interaction and that are important for partner recognition. The pseudoazurin structure was compared with other structures of various type 1 copper proteins and these were grouped into families according to similarities in their secondary structure; this may be useful in the annotation of copper proteins in newly sequenced genomes and in the identification of novel copper proteins.DQ - Departamento de QuímicaCQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE)RUNNajmudin, ShabirPauleta, Sofia R.Moura, IsabelRomão, Maria J.2019-03-11T23:15:17Z2010-06-102010-06-10T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttps://doi.org/10.1107/S1744309110013989eng1744-3091PURE: 12010412http://www.scopus.com/inward/record.url?scp=77953142481&partnerID=8YFLogxKhttps://doi.org/10.1107/S1744309110013989info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:29:46Zoai:run.unl.pt:10362/62995Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:33:50.009667Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The 1.4 Å resolution structure of Paracoccus pantotrophus pseudoazurin |
title |
The 1.4 Å resolution structure of Paracoccus pantotrophus pseudoazurin |
spellingShingle |
The 1.4 Å resolution structure of Paracoccus pantotrophus pseudoazurin Najmudin, Shabir Copper proteins Electron transfer Paracoccus pantotrophus Pseudoazurin Type 1 blue copper protein family Biophysics Structural Biology Biochemistry Genetics Condensed Matter Physics |
title_short |
The 1.4 Å resolution structure of Paracoccus pantotrophus pseudoazurin |
title_full |
The 1.4 Å resolution structure of Paracoccus pantotrophus pseudoazurin |
title_fullStr |
The 1.4 Å resolution structure of Paracoccus pantotrophus pseudoazurin |
title_full_unstemmed |
The 1.4 Å resolution structure of Paracoccus pantotrophus pseudoazurin |
title_sort |
The 1.4 Å resolution structure of Paracoccus pantotrophus pseudoazurin |
author |
Najmudin, Shabir |
author_facet |
Najmudin, Shabir Pauleta, Sofia R. Moura, Isabel Romão, Maria J. |
author_role |
author |
author2 |
Pauleta, Sofia R. Moura, Isabel Romão, Maria J. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
DQ - Departamento de Química CQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE) RUN |
dc.contributor.author.fl_str_mv |
Najmudin, Shabir Pauleta, Sofia R. Moura, Isabel Romão, Maria J. |
dc.subject.por.fl_str_mv |
Copper proteins Electron transfer Paracoccus pantotrophus Pseudoazurin Type 1 blue copper protein family Biophysics Structural Biology Biochemistry Genetics Condensed Matter Physics |
topic |
Copper proteins Electron transfer Paracoccus pantotrophus Pseudoazurin Type 1 blue copper protein family Biophysics Structural Biology Biochemistry Genetics Condensed Matter Physics |
description |
This work was supported in part by Fundação para a Ciência e a Tecnologia (Lisbon, Portugal) through project grant POCTI/QUI/64638/2006 and individual grant SFRH/BPD/20357/2004 (SN). The authors would like to thank Dr Cecilia Bonifacio for help with crystallization and Dr Jose Trincao for help with data collection. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-06-10 2010-06-10T00:00:00Z 2019-03-11T23:15:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1107/S1744309110013989 |
url |
https://doi.org/10.1107/S1744309110013989 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1744-3091 PURE: 12010412 http://www.scopus.com/inward/record.url?scp=77953142481&partnerID=8YFLogxK https://doi.org/10.1107/S1744309110013989 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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9 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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