Denervation impairs regeneration of amputated zebrafish fins

Detalhes bibliográficos
Autor(a) principal: Simões, Mariana G.
Data de Publicação: 2014
Outros Autores: Bensimon-Brito, Anabela, Fonseca, Mariana, Farinho, Ana, Valério, F., Afonso, Nuno, Kumar, A., Jacinto, António
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/24234
Resumo: info:eu-repo/grantAgreement/FCT/3599-PPCDT/126111/PT We thank to the IMM Fish Facility manager Lara Carvalho for support with fish care, to the CEDOC Fish Facility, to the IMM Bioimaging Unit and to the IGC Unit of Imaging and Cytometry. We thank to Leonor Saude, Henry Roehl and Kenneth Poss for providing probes. We thank Jeremy Brockes, Jacqueline Geraudie, Rita Fior, Elsa Abranches, Joaquin Rodriguez Leon, Nuno Afonso, Rita Mateus and Pedro Alves for reading the manuscript and for insightful discussions that lead to this work. Mariana Guedes Simoes was supported by a PhD grant from Fundacao para a Ciencia e Tecnologia (FCT), Portugal. Anabela Bensimon-Brito was suported by the ERC Reseal project (2007-StG-208631). The research presented in this manuscript has been funded by FCT research grants (PTDC/SAU-GMG/101537/2008 PTDC/SAU-OBD/100200/2008 PTDC/BEX-BID/1176/2012).
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spelling Denervation impairs regeneration of amputated zebrafish finsIN-SITU HYBRIDIZATIONURODELE LIMBPECTORAL FINGENE-EXPRESSIONAMPHIBIAN LIMB REGENERATIONRETINOIC ACIDDANIO-RERIOAMBYSTOMA-MEXICANUMNERVE DEPENDENCESONIC-HEDGEHOGinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/126111/PT We thank to the IMM Fish Facility manager Lara Carvalho for support with fish care, to the CEDOC Fish Facility, to the IMM Bioimaging Unit and to the IGC Unit of Imaging and Cytometry. We thank to Leonor Saude, Henry Roehl and Kenneth Poss for providing probes. We thank Jeremy Brockes, Jacqueline Geraudie, Rita Fior, Elsa Abranches, Joaquin Rodriguez Leon, Nuno Afonso, Rita Mateus and Pedro Alves for reading the manuscript and for insightful discussions that lead to this work. Mariana Guedes Simoes was supported by a PhD grant from Fundacao para a Ciencia e Tecnologia (FCT), Portugal. Anabela Bensimon-Brito was suported by the ERC Reseal project (2007-StG-208631). The research presented in this manuscript has been funded by FCT research grants (PTDC/SAU-GMG/101537/2008 PTDC/SAU-OBD/100200/2008 PTDC/BEX-BID/1176/2012).Background: Zebrafish are able to regenerate many of its tissues and organs after damage. In amphibians this process is regulated by nerve fibres present at the site of injury, which have been proposed to release factors into the amputated limbs/fins, promoting and sustaining the proliferation of blastemal cells. Although some candidate factors have been proposed to mediate the nerve dependency of regeneration, the molecular mechanisms involved in this process remain unclear. Results: We have used zebrafish as a model system to address the role of nerve fibres in fin regeneration. We have developed a protocol for pectoral fin denervation followed by amputation and analysed the regenerative process under this experimental conditions. Upon denervation fins were able to close the wound and form a wound epidermis, but could not establish a functional apical epithelial cap, with a posterior failure of blastema formation and outgrowth, and the accumulation of several defects. The expression patterns of genes known to be key players during fin regeneration were altered upon denervation, suggesting that nerves can contribute to the regulation of the Fgf, Wnt and Shh pathways during zebrafish fin regeneration. Conclusions: Our results demonstrate that proper innervation of the zebrafish pectoral fin is essential for a successful regenerative process, and establish this organism as a useful model to understand the molecular and cellular mechanisms of nerve dependence, during vertebrate regeneration.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNSimões, Mariana G.Bensimon-Brito, AnabelaFonseca, MarianaFarinho, AnaValério, F.Afonso, NunoKumar, A.Jacinto, António2017-10-16T22:01:49Z2014-122014-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article21application/pdfhttp://hdl.handle.net/10362/24234eng1471-213XPURE: 459207https://doi.org/10.1186/s12861-014-0049-2info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:12:34Zoai:run.unl.pt:10362/24234Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:28:01.158518Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Denervation impairs regeneration of amputated zebrafish fins
title Denervation impairs regeneration of amputated zebrafish fins
spellingShingle Denervation impairs regeneration of amputated zebrafish fins
Simões, Mariana G.
IN-SITU HYBRIDIZATION
URODELE LIMB
PECTORAL FIN
GENE-EXPRESSION
AMPHIBIAN LIMB REGENERATION
RETINOIC ACID
DANIO-RERIO
AMBYSTOMA-MEXICANUM
NERVE DEPENDENCE
SONIC-HEDGEHOG
title_short Denervation impairs regeneration of amputated zebrafish fins
title_full Denervation impairs regeneration of amputated zebrafish fins
title_fullStr Denervation impairs regeneration of amputated zebrafish fins
title_full_unstemmed Denervation impairs regeneration of amputated zebrafish fins
title_sort Denervation impairs regeneration of amputated zebrafish fins
author Simões, Mariana G.
author_facet Simões, Mariana G.
Bensimon-Brito, Anabela
Fonseca, Mariana
Farinho, Ana
Valério, F.
Afonso, Nuno
Kumar, A.
Jacinto, António
author_role author
author2 Bensimon-Brito, Anabela
Fonseca, Mariana
Farinho, Ana
Valério, F.
Afonso, Nuno
Kumar, A.
Jacinto, António
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Simões, Mariana G.
Bensimon-Brito, Anabela
Fonseca, Mariana
Farinho, Ana
Valério, F.
Afonso, Nuno
Kumar, A.
Jacinto, António
dc.subject.por.fl_str_mv IN-SITU HYBRIDIZATION
URODELE LIMB
PECTORAL FIN
GENE-EXPRESSION
AMPHIBIAN LIMB REGENERATION
RETINOIC ACID
DANIO-RERIO
AMBYSTOMA-MEXICANUM
NERVE DEPENDENCE
SONIC-HEDGEHOG
topic IN-SITU HYBRIDIZATION
URODELE LIMB
PECTORAL FIN
GENE-EXPRESSION
AMPHIBIAN LIMB REGENERATION
RETINOIC ACID
DANIO-RERIO
AMBYSTOMA-MEXICANUM
NERVE DEPENDENCE
SONIC-HEDGEHOG
description info:eu-repo/grantAgreement/FCT/3599-PPCDT/126111/PT We thank to the IMM Fish Facility manager Lara Carvalho for support with fish care, to the CEDOC Fish Facility, to the IMM Bioimaging Unit and to the IGC Unit of Imaging and Cytometry. We thank to Leonor Saude, Henry Roehl and Kenneth Poss for providing probes. We thank Jeremy Brockes, Jacqueline Geraudie, Rita Fior, Elsa Abranches, Joaquin Rodriguez Leon, Nuno Afonso, Rita Mateus and Pedro Alves for reading the manuscript and for insightful discussions that lead to this work. Mariana Guedes Simoes was supported by a PhD grant from Fundacao para a Ciencia e Tecnologia (FCT), Portugal. Anabela Bensimon-Brito was suported by the ERC Reseal project (2007-StG-208631). The research presented in this manuscript has been funded by FCT research grants (PTDC/SAU-GMG/101537/2008 PTDC/SAU-OBD/100200/2008 PTDC/BEX-BID/1176/2012).
publishDate 2014
dc.date.none.fl_str_mv 2014-12
2014-12-01T00:00:00Z
2017-10-16T22:01:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/24234
url http://hdl.handle.net/10362/24234
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1471-213X
PURE: 459207
https://doi.org/10.1186/s12861-014-0049-2
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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