Ady2p is essential for the acetate permease activity in the yeast Saccharomyces cerevisiae
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/2935 |
Resumo: | To identify new genes involved in acetate uptake in Saccharomyces cerevisiae, an analysis of the gene expression profiles of cells shifted from glucose to acetic acid was performed. The gene expression reprogramming of yeast adapting to a poor non-fermentable carbon source was observed, including dramatic metabolic changes, global activation of translation machinery, mitochondria biogenesis and the induction of known or putative transporters. Among them, the gene ADY2/YCR010c was identified as a new key element for acetate transport, being homologous to the Yarrowia lipolytica GPR1 gene, which has a role in acetic acid sensitivity. Disruption of ADY2 in S. cerevisiae abolished the active transport of acetate. Microarray analyses of ady2 strains showed that this gene is not a critical regulator of acetate response and that its role is directly connected to acetate transport. Ady2p is predicted to be a membrane protein and is a valuable acetate transporter candidate. |
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Ady2p is essential for the acetate permease activity in the yeast Saccharomyces cerevisiaeMonocarboxylate transportYeastAcetateGene expressionGPR1Science & TechnologyTo identify new genes involved in acetate uptake in Saccharomyces cerevisiae, an analysis of the gene expression profiles of cells shifted from glucose to acetic acid was performed. The gene expression reprogramming of yeast adapting to a poor non-fermentable carbon source was observed, including dramatic metabolic changes, global activation of translation machinery, mitochondria biogenesis and the induction of known or putative transporters. Among them, the gene ADY2/YCR010c was identified as a new key element for acetate transport, being homologous to the Yarrowia lipolytica GPR1 gene, which has a role in acetic acid sensitivity. Disruption of ADY2 in S. cerevisiae abolished the active transport of acetate. Microarray analyses of ady2 strains showed that this gene is not a critical regulator of acetate response and that its role is directly connected to acetate transport. Ady2p is predicted to be a membrane protein and is a valuable acetate transporter candidate.John Wiley and SonsUniversidade do MinhoPaiva, SandraDevaux, FredericBarbosa, SóniaJacq, ClaudeCasal, Margarida2004-022004-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/2935eng"Yeast". ISSN 1496-8426. 21:3 (2004) 201-210.1496-842610.1002/yea.105614968426http://www3.interscience.wiley.com/cgi-bin/fulltext/107612571/PDFSTARTinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:02:29Zoai:repositorium.sdum.uminho.pt:1822/2935Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:52:27.370020Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Ady2p is essential for the acetate permease activity in the yeast Saccharomyces cerevisiae |
title |
Ady2p is essential for the acetate permease activity in the yeast Saccharomyces cerevisiae |
spellingShingle |
Ady2p is essential for the acetate permease activity in the yeast Saccharomyces cerevisiae Paiva, Sandra Monocarboxylate transport Yeast Acetate Gene expression GPR1 Science & Technology |
title_short |
Ady2p is essential for the acetate permease activity in the yeast Saccharomyces cerevisiae |
title_full |
Ady2p is essential for the acetate permease activity in the yeast Saccharomyces cerevisiae |
title_fullStr |
Ady2p is essential for the acetate permease activity in the yeast Saccharomyces cerevisiae |
title_full_unstemmed |
Ady2p is essential for the acetate permease activity in the yeast Saccharomyces cerevisiae |
title_sort |
Ady2p is essential for the acetate permease activity in the yeast Saccharomyces cerevisiae |
author |
Paiva, Sandra |
author_facet |
Paiva, Sandra Devaux, Frederic Barbosa, Sónia Jacq, Claude Casal, Margarida |
author_role |
author |
author2 |
Devaux, Frederic Barbosa, Sónia Jacq, Claude Casal, Margarida |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Paiva, Sandra Devaux, Frederic Barbosa, Sónia Jacq, Claude Casal, Margarida |
dc.subject.por.fl_str_mv |
Monocarboxylate transport Yeast Acetate Gene expression GPR1 Science & Technology |
topic |
Monocarboxylate transport Yeast Acetate Gene expression GPR1 Science & Technology |
description |
To identify new genes involved in acetate uptake in Saccharomyces cerevisiae, an analysis of the gene expression profiles of cells shifted from glucose to acetic acid was performed. The gene expression reprogramming of yeast adapting to a poor non-fermentable carbon source was observed, including dramatic metabolic changes, global activation of translation machinery, mitochondria biogenesis and the induction of known or putative transporters. Among them, the gene ADY2/YCR010c was identified as a new key element for acetate transport, being homologous to the Yarrowia lipolytica GPR1 gene, which has a role in acetic acid sensitivity. Disruption of ADY2 in S. cerevisiae abolished the active transport of acetate. Microarray analyses of ady2 strains showed that this gene is not a critical regulator of acetate response and that its role is directly connected to acetate transport. Ady2p is predicted to be a membrane protein and is a valuable acetate transporter candidate. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-02 2004-02-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/2935 |
url |
http://hdl.handle.net/1822/2935 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Yeast". ISSN 1496-8426. 21:3 (2004) 201-210. 1496-8426 10.1002/yea.1056 14968426 http://www3.interscience.wiley.com/cgi-bin/fulltext/107612571/PDFSTART |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
John Wiley and Sons |
publisher.none.fl_str_mv |
John Wiley and Sons |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132301038190592 |