Development of films based on bacterial biopolyesters produced from apple pulp waste
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/55076 |
Resumo: | The first objective of this thesis was to use apple pulp waste, a leftover from the fruit processing industry, for the cultivation of Pseudomonas citronellolis NNRL B-2504, Cupriavidus necator DSM 428 and a co-culture using both strains, culminating on the production of medium-chain-length polyhydroxyalkanoates (mcl-PHAs), short-chain-length polyhydroxyalkanoates (scl-PHAs) and a natural biosynthesized blend, respectively. PHAs are biopolyesters with versatile material properties, applicable in several fields. The soluble fraction of the apple pulp waste, rich in fructose, sucrose and glucose, was used for the batch bioreactor cultivation of P. citronellolis and C. necator. The first cultivation, P. citronellolis, reached a polymer content in the biomass of 30wt.% with a volumetric productivity of 0.61 g/L.day. The polymer was mainly composed of 3-hydroxydecanoate (68%) and 3-hydroxyoctanoate (22%), and had a molecular weight of 3.7×105 Da. It presented melting and thermal degradation temperatures of 53 and 296 °C, respectively, and a crystallinity degree of 15%. C. necator DSM 428 achieved a volumetric productivity of 1.59 g/L.day, reaching a polymer content 44 wt.% . The homopolymer, P(3HB) was obtained, with a molecular weight of 5.0×105 Da and a crystallinity degree of 43%. Moreover, the polymer had melting and thermal degradation temperatures of 179 and 293 °C, respectively. Finally, a third batch production using a co-culture was performed. The production presented a 34 wt.% of polymer content with a volumetric productivity of 0.96 g/L.day. The polymer produced was a physical mixture of mcl-PHA and P(3HB). These results demonstrated that apple pulp waste is a suitable feedstock for the production of different biopolymers with distinct physical-chemical properties. The aim of the second part of this thesis was to prepare films based on the PHAs produced from apple pulp waste, followed by their characterization, both physical and chemical. All films presented similar thermal, crystallinity and chemical features when compared to the neat polymer, which indicates that the procedure did not significantly impact on the polymers’ characteristics. Gas permeation tests were performed for both oxygen and carbon dioxide. mcl-PHA films presented the best results for both O2 and CO2 (1.1×10-09 and 5.3×10-09 cm3.cm/cm2.s.cmHg, respectively). The mechanical tests performed for all PHA films revealed that mcl-PHA and blends demonstrated higher deformation until break, and P(3HB) had superior tensile strength. |
id |
RCAP_65efef398bcaa02fd8aec9488a4fe4f0 |
---|---|
oai_identifier_str |
oai:run.unl.pt:10362/55076 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Development of films based on bacterial biopolyesters produced from apple pulp wasteApple pulp wasteshort-chain length polyhydroxyalkanoates (scl-PHA)medium-chain length polyhydroxyalkanoates (mcl-PHA)polymer blendsfilmsDomínio/Área Científica::Engenharia e Tecnologia::Engenharia QuímicaThe first objective of this thesis was to use apple pulp waste, a leftover from the fruit processing industry, for the cultivation of Pseudomonas citronellolis NNRL B-2504, Cupriavidus necator DSM 428 and a co-culture using both strains, culminating on the production of medium-chain-length polyhydroxyalkanoates (mcl-PHAs), short-chain-length polyhydroxyalkanoates (scl-PHAs) and a natural biosynthesized blend, respectively. PHAs are biopolyesters with versatile material properties, applicable in several fields. The soluble fraction of the apple pulp waste, rich in fructose, sucrose and glucose, was used for the batch bioreactor cultivation of P. citronellolis and C. necator. The first cultivation, P. citronellolis, reached a polymer content in the biomass of 30wt.% with a volumetric productivity of 0.61 g/L.day. The polymer was mainly composed of 3-hydroxydecanoate (68%) and 3-hydroxyoctanoate (22%), and had a molecular weight of 3.7×105 Da. It presented melting and thermal degradation temperatures of 53 and 296 °C, respectively, and a crystallinity degree of 15%. C. necator DSM 428 achieved a volumetric productivity of 1.59 g/L.day, reaching a polymer content 44 wt.% . The homopolymer, P(3HB) was obtained, with a molecular weight of 5.0×105 Da and a crystallinity degree of 43%. Moreover, the polymer had melting and thermal degradation temperatures of 179 and 293 °C, respectively. Finally, a third batch production using a co-culture was performed. The production presented a 34 wt.% of polymer content with a volumetric productivity of 0.96 g/L.day. The polymer produced was a physical mixture of mcl-PHA and P(3HB). These results demonstrated that apple pulp waste is a suitable feedstock for the production of different biopolymers with distinct physical-chemical properties. The aim of the second part of this thesis was to prepare films based on the PHAs produced from apple pulp waste, followed by their characterization, both physical and chemical. All films presented similar thermal, crystallinity and chemical features when compared to the neat polymer, which indicates that the procedure did not significantly impact on the polymers’ characteristics. Gas permeation tests were performed for both oxygen and carbon dioxide. mcl-PHA films presented the best results for both O2 and CO2 (1.1×10-09 and 5.3×10-09 cm3.cm/cm2.s.cmHg, respectively). The mechanical tests performed for all PHA films revealed that mcl-PHA and blends demonstrated higher deformation until break, and P(3HB) had superior tensile strength.Freitas, FilomenaNeves, LuísaRUNRebocho, Ana Teresa Mataloto2018-12-19T15:48:59Z2018-1120182018-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/55076enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:26:56Zoai:run.unl.pt:10362/55076Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:32:49.845239Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Development of films based on bacterial biopolyesters produced from apple pulp waste |
title |
Development of films based on bacterial biopolyesters produced from apple pulp waste |
spellingShingle |
Development of films based on bacterial biopolyesters produced from apple pulp waste Rebocho, Ana Teresa Mataloto Apple pulp waste short-chain length polyhydroxyalkanoates (scl-PHA) medium-chain length polyhydroxyalkanoates (mcl-PHA) polymer blends films Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Química |
title_short |
Development of films based on bacterial biopolyesters produced from apple pulp waste |
title_full |
Development of films based on bacterial biopolyesters produced from apple pulp waste |
title_fullStr |
Development of films based on bacterial biopolyesters produced from apple pulp waste |
title_full_unstemmed |
Development of films based on bacterial biopolyesters produced from apple pulp waste |
title_sort |
Development of films based on bacterial biopolyesters produced from apple pulp waste |
author |
Rebocho, Ana Teresa Mataloto |
author_facet |
Rebocho, Ana Teresa Mataloto |
author_role |
author |
dc.contributor.none.fl_str_mv |
Freitas, Filomena Neves, Luísa RUN |
dc.contributor.author.fl_str_mv |
Rebocho, Ana Teresa Mataloto |
dc.subject.por.fl_str_mv |
Apple pulp waste short-chain length polyhydroxyalkanoates (scl-PHA) medium-chain length polyhydroxyalkanoates (mcl-PHA) polymer blends films Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Química |
topic |
Apple pulp waste short-chain length polyhydroxyalkanoates (scl-PHA) medium-chain length polyhydroxyalkanoates (mcl-PHA) polymer blends films Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Química |
description |
The first objective of this thesis was to use apple pulp waste, a leftover from the fruit processing industry, for the cultivation of Pseudomonas citronellolis NNRL B-2504, Cupriavidus necator DSM 428 and a co-culture using both strains, culminating on the production of medium-chain-length polyhydroxyalkanoates (mcl-PHAs), short-chain-length polyhydroxyalkanoates (scl-PHAs) and a natural biosynthesized blend, respectively. PHAs are biopolyesters with versatile material properties, applicable in several fields. The soluble fraction of the apple pulp waste, rich in fructose, sucrose and glucose, was used for the batch bioreactor cultivation of P. citronellolis and C. necator. The first cultivation, P. citronellolis, reached a polymer content in the biomass of 30wt.% with a volumetric productivity of 0.61 g/L.day. The polymer was mainly composed of 3-hydroxydecanoate (68%) and 3-hydroxyoctanoate (22%), and had a molecular weight of 3.7×105 Da. It presented melting and thermal degradation temperatures of 53 and 296 °C, respectively, and a crystallinity degree of 15%. C. necator DSM 428 achieved a volumetric productivity of 1.59 g/L.day, reaching a polymer content 44 wt.% . The homopolymer, P(3HB) was obtained, with a molecular weight of 5.0×105 Da and a crystallinity degree of 43%. Moreover, the polymer had melting and thermal degradation temperatures of 179 and 293 °C, respectively. Finally, a third batch production using a co-culture was performed. The production presented a 34 wt.% of polymer content with a volumetric productivity of 0.96 g/L.day. The polymer produced was a physical mixture of mcl-PHA and P(3HB). These results demonstrated that apple pulp waste is a suitable feedstock for the production of different biopolymers with distinct physical-chemical properties. The aim of the second part of this thesis was to prepare films based on the PHAs produced from apple pulp waste, followed by their characterization, both physical and chemical. All films presented similar thermal, crystallinity and chemical features when compared to the neat polymer, which indicates that the procedure did not significantly impact on the polymers’ characteristics. Gas permeation tests were performed for both oxygen and carbon dioxide. mcl-PHA films presented the best results for both O2 and CO2 (1.1×10-09 and 5.3×10-09 cm3.cm/cm2.s.cmHg, respectively). The mechanical tests performed for all PHA films revealed that mcl-PHA and blends demonstrated higher deformation until break, and P(3HB) had superior tensile strength. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-19T15:48:59Z 2018-11 2018 2018-11-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/55076 |
url |
http://hdl.handle.net/10362/55076 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799137950185816064 |