Serine-based surfactants as effective antimicrobial agents against multiresistant bacteria
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/20583 |
Resumo: | The antimicrobial activity of two serine derived gemini cationic surfactants, amide (12Ser)2CON12 and ester (12Ser)2COO12, was tested using sensitive, E. coli ATCC 25922 and S. aureus ATCC 6538, and resistant, E. coli CTX M2, E. coli TEM CTX M9 and S. aureus ATCC 6538 and S. aureus MRSA ATCC 43300 Gram-positive and Gram-negative bacteria strains. Very low MIC values (5 μM) were found for the two resistant strains E.coli TEM CTX M9 and S. aureus MRSA ATCC 43300, in the case of the amide derivative, and for S. aureus MRSA ATCC 43300, in the case of the ester derivative. The interaction of the serine amphiphiles with lipid-model membranes (DPPG and DPPC) was investigated using Langmuir monolayers. A more pronounced effect on the DPPG than on the DPPC monolayer was observed. The effect induced by the surfactants on bacteria membrane was explored by Atomic Force Microscopy. A clear disruption of the bacteria membrane was observed for E. coli TEM CTX M9 upon treatment with (12ser)2CON12, whereas for the S. aureus MRSA few observable changes in cell morphology were found after treatment with either of the two surfactants. The cytotoxicity of the two compounds was assessed by hemolysis assay on human red blood cells (RBC). The compounds were shown to be non-cytotoxic up to 10 μM. Overall, the results reveal a promising potential, in particular of the amide derivative, as antimicrobial agent for two strains of antibiotic resistant bacteria. |
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Serine-based surfactants as effective antimicrobial agents against multiresistant bacteriaGemini surfactantsSerineAntimicrobial activityLangmuir monolayersHemolytic activityThe antimicrobial activity of two serine derived gemini cationic surfactants, amide (12Ser)2CON12 and ester (12Ser)2COO12, was tested using sensitive, E. coli ATCC 25922 and S. aureus ATCC 6538, and resistant, E. coli CTX M2, E. coli TEM CTX M9 and S. aureus ATCC 6538 and S. aureus MRSA ATCC 43300 Gram-positive and Gram-negative bacteria strains. Very low MIC values (5 μM) were found for the two resistant strains E.coli TEM CTX M9 and S. aureus MRSA ATCC 43300, in the case of the amide derivative, and for S. aureus MRSA ATCC 43300, in the case of the ester derivative. The interaction of the serine amphiphiles with lipid-model membranes (DPPG and DPPC) was investigated using Langmuir monolayers. A more pronounced effect on the DPPG than on the DPPC monolayer was observed. The effect induced by the surfactants on bacteria membrane was explored by Atomic Force Microscopy. A clear disruption of the bacteria membrane was observed for E. coli TEM CTX M9 upon treatment with (12ser)2CON12, whereas for the S. aureus MRSA few observable changes in cell morphology were found after treatment with either of the two surfactants. The cytotoxicity of the two compounds was assessed by hemolysis assay on human red blood cells (RBC). The compounds were shown to be non-cytotoxic up to 10 μM. Overall, the results reveal a promising potential, in particular of the amide derivative, as antimicrobial agent for two strains of antibiotic resistant bacteria.ElsevierRepositório Científico do Instituto Politécnico do PortoSilva, Sandra G.Pinheiro, MarinaPereira, RuiDias, Ana RitaFerraz, RicardoPrudêncio, CristinaEaton, Peter J.Reis, SaletteVale, M. Luísa C. do2022-06-02T15:03:51Z2022-05-162022-05-16T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdftext/plainhttp://hdl.handle.net/10400.22/20583engSilva, S. G., Pinheiro, M., Pereira, R., Dias, A. R., Ferraz, R., Prudêncio, C., Eaton, P. J., Reis, S., & do Vale, M. L. C. (2022). Serine-based surfactants as effective antimicrobial agents against multiresistant bacteria. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1864(9), 183969. https://doi.org/https://doi.org/10.1016/j.bbamem.2022.1839690005-273610.1016/j.bbamem.2022.1839691879-2642metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T13:16:05Zoai:recipp.ipp.pt:10400.22/20583Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:40:37.577742Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Serine-based surfactants as effective antimicrobial agents against multiresistant bacteria |
title |
Serine-based surfactants as effective antimicrobial agents against multiresistant bacteria |
spellingShingle |
Serine-based surfactants as effective antimicrobial agents against multiresistant bacteria Silva, Sandra G. Gemini surfactants Serine Antimicrobial activity Langmuir monolayers Hemolytic activity |
title_short |
Serine-based surfactants as effective antimicrobial agents against multiresistant bacteria |
title_full |
Serine-based surfactants as effective antimicrobial agents against multiresistant bacteria |
title_fullStr |
Serine-based surfactants as effective antimicrobial agents against multiresistant bacteria |
title_full_unstemmed |
Serine-based surfactants as effective antimicrobial agents against multiresistant bacteria |
title_sort |
Serine-based surfactants as effective antimicrobial agents against multiresistant bacteria |
author |
Silva, Sandra G. |
author_facet |
Silva, Sandra G. Pinheiro, Marina Pereira, Rui Dias, Ana Rita Ferraz, Ricardo Prudêncio, Cristina Eaton, Peter J. Reis, Salette Vale, M. Luísa C. do |
author_role |
author |
author2 |
Pinheiro, Marina Pereira, Rui Dias, Ana Rita Ferraz, Ricardo Prudêncio, Cristina Eaton, Peter J. Reis, Salette Vale, M. Luísa C. do |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Silva, Sandra G. Pinheiro, Marina Pereira, Rui Dias, Ana Rita Ferraz, Ricardo Prudêncio, Cristina Eaton, Peter J. Reis, Salette Vale, M. Luísa C. do |
dc.subject.por.fl_str_mv |
Gemini surfactants Serine Antimicrobial activity Langmuir monolayers Hemolytic activity |
topic |
Gemini surfactants Serine Antimicrobial activity Langmuir monolayers Hemolytic activity |
description |
The antimicrobial activity of two serine derived gemini cationic surfactants, amide (12Ser)2CON12 and ester (12Ser)2COO12, was tested using sensitive, E. coli ATCC 25922 and S. aureus ATCC 6538, and resistant, E. coli CTX M2, E. coli TEM CTX M9 and S. aureus ATCC 6538 and S. aureus MRSA ATCC 43300 Gram-positive and Gram-negative bacteria strains. Very low MIC values (5 μM) were found for the two resistant strains E.coli TEM CTX M9 and S. aureus MRSA ATCC 43300, in the case of the amide derivative, and for S. aureus MRSA ATCC 43300, in the case of the ester derivative. The interaction of the serine amphiphiles with lipid-model membranes (DPPG and DPPC) was investigated using Langmuir monolayers. A more pronounced effect on the DPPG than on the DPPC monolayer was observed. The effect induced by the surfactants on bacteria membrane was explored by Atomic Force Microscopy. A clear disruption of the bacteria membrane was observed for E. coli TEM CTX M9 upon treatment with (12ser)2CON12, whereas for the S. aureus MRSA few observable changes in cell morphology were found after treatment with either of the two surfactants. The cytotoxicity of the two compounds was assessed by hemolysis assay on human red blood cells (RBC). The compounds were shown to be non-cytotoxic up to 10 μM. Overall, the results reveal a promising potential, in particular of the amide derivative, as antimicrobial agent for two strains of antibiotic resistant bacteria. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-06-02T15:03:51Z 2022-05-16 2022-05-16T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/20583 |
url |
http://hdl.handle.net/10400.22/20583 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Silva, S. G., Pinheiro, M., Pereira, R., Dias, A. R., Ferraz, R., Prudêncio, C., Eaton, P. J., Reis, S., & do Vale, M. L. C. (2022). Serine-based surfactants as effective antimicrobial agents against multiresistant bacteria. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1864(9), 183969. https://doi.org/https://doi.org/10.1016/j.bbamem.2022.183969 0005-2736 10.1016/j.bbamem.2022.183969 1879-2642 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
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metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/plain |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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