Analysis of the expression of putative heat-stress related genes in relation to thermotolerance of cork oak
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/20.500.12207/679 |
Resumo: | Cork oak (Quercus suber L.) is a research priority in the Mediterranean area and because of cork oaks’ dis-tribution these stands are experiencing daily stress. Based on projections of intensifying climate change and considering the key role of exploring the recovery abilities, cork oak seedlings were subjected to a cumulative temperature increase from 25 ◦C to 55 ◦C and subsequent recovery. CO2 assimilation rate, chlorophyll fluorescence, anthocyanins, proline and lipid peroxidation were used to evaluate plant per-formance, while the relative abundance of seven genes encoding for proteins of cork oak with a putative role in thermal/stress regulation (POX1, POX2, HSP10.4, HSP17a.22, CHS, MTL and RBC) was analyzed by qPCR (quantitative Polymerase Chain Reaction). A temperature change to 35 ◦C showed abundance alterations in the tested genes; at 45 ◦C, the molecular changes were associated with an antioxidant response, possibly modulated by anthocyanins. At 55 ◦C, HSP17a.22, MTL and proline accumulation were evident. After recovery, physiological balance was restored, whereas POX1, HSP10.4 and MTL abundances were suggested to be involved in increased thermotolerance. The data presented here are expected to pinpoint some pathways changes occurring during such stress and further recovery in this particular Mediterranean species. |
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Analysis of the expression of putative heat-stress related genes in relation to thermotolerance of cork oakHigh temperaturePlant performanceQuercus suberRecoveryCork oak (Quercus suber L.) is a research priority in the Mediterranean area and because of cork oaks’ dis-tribution these stands are experiencing daily stress. Based on projections of intensifying climate change and considering the key role of exploring the recovery abilities, cork oak seedlings were subjected to a cumulative temperature increase from 25 ◦C to 55 ◦C and subsequent recovery. CO2 assimilation rate, chlorophyll fluorescence, anthocyanins, proline and lipid peroxidation were used to evaluate plant per-formance, while the relative abundance of seven genes encoding for proteins of cork oak with a putative role in thermal/stress regulation (POX1, POX2, HSP10.4, HSP17a.22, CHS, MTL and RBC) was analyzed by qPCR (quantitative Polymerase Chain Reaction). A temperature change to 35 ◦C showed abundance alterations in the tested genes; at 45 ◦C, the molecular changes were associated with an antioxidant response, possibly modulated by anthocyanins. At 55 ◦C, HSP17a.22, MTL and proline accumulation were evident. After recovery, physiological balance was restored, whereas POX1, HSP10.4 and MTL abundances were suggested to be involved in increased thermotolerance. The data presented here are expected to pinpoint some pathways changes occurring during such stress and further recovery in this particular Mediterranean species.Elsevier2014-02-25T13:04:48Z2014-02-24T00:00:00Z2014-03-15T00:00:00Z2014-03-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/20.500.12207/679engmetadata only accessinfo:eu-repo/semantics/openAccessCorreia, BarbaraRodriguez, José LuisValledor, LuisAlmeida, TâniaSantos, ConceiçãoCanal, Maria JesúsPinto, Glóriareponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-06-23T07:46:38Zoai:repositorio.ipbeja.pt:20.500.12207/679Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T14:58:25.816590Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Analysis of the expression of putative heat-stress related genes in relation to thermotolerance of cork oak |
title |
Analysis of the expression of putative heat-stress related genes in relation to thermotolerance of cork oak |
spellingShingle |
Analysis of the expression of putative heat-stress related genes in relation to thermotolerance of cork oak Correia, Barbara High temperature Plant performance Quercus suber Recovery |
title_short |
Analysis of the expression of putative heat-stress related genes in relation to thermotolerance of cork oak |
title_full |
Analysis of the expression of putative heat-stress related genes in relation to thermotolerance of cork oak |
title_fullStr |
Analysis of the expression of putative heat-stress related genes in relation to thermotolerance of cork oak |
title_full_unstemmed |
Analysis of the expression of putative heat-stress related genes in relation to thermotolerance of cork oak |
title_sort |
Analysis of the expression of putative heat-stress related genes in relation to thermotolerance of cork oak |
author |
Correia, Barbara |
author_facet |
Correia, Barbara Rodriguez, José Luis Valledor, Luis Almeida, Tânia Santos, Conceição Canal, Maria Jesús Pinto, Glória |
author_role |
author |
author2 |
Rodriguez, José Luis Valledor, Luis Almeida, Tânia Santos, Conceição Canal, Maria Jesús Pinto, Glória |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Correia, Barbara Rodriguez, José Luis Valledor, Luis Almeida, Tânia Santos, Conceição Canal, Maria Jesús Pinto, Glória |
dc.subject.por.fl_str_mv |
High temperature Plant performance Quercus suber Recovery |
topic |
High temperature Plant performance Quercus suber Recovery |
description |
Cork oak (Quercus suber L.) is a research priority in the Mediterranean area and because of cork oaks’ dis-tribution these stands are experiencing daily stress. Based on projections of intensifying climate change and considering the key role of exploring the recovery abilities, cork oak seedlings were subjected to a cumulative temperature increase from 25 ◦C to 55 ◦C and subsequent recovery. CO2 assimilation rate, chlorophyll fluorescence, anthocyanins, proline and lipid peroxidation were used to evaluate plant per-formance, while the relative abundance of seven genes encoding for proteins of cork oak with a putative role in thermal/stress regulation (POX1, POX2, HSP10.4, HSP17a.22, CHS, MTL and RBC) was analyzed by qPCR (quantitative Polymerase Chain Reaction). A temperature change to 35 ◦C showed abundance alterations in the tested genes; at 45 ◦C, the molecular changes were associated with an antioxidant response, possibly modulated by anthocyanins. At 55 ◦C, HSP17a.22, MTL and proline accumulation were evident. After recovery, physiological balance was restored, whereas POX1, HSP10.4 and MTL abundances were suggested to be involved in increased thermotolerance. The data presented here are expected to pinpoint some pathways changes occurring during such stress and further recovery in this particular Mediterranean species. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02-25T13:04:48Z 2014-02-24T00:00:00Z 2014-03-15T00:00:00Z 2014-03-15T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/20.500.12207/679 |
url |
http://hdl.handle.net/20.500.12207/679 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799129857985085440 |