Ghrelin expression in human and rat fetal lungs and the effect of ghrelin administration in nitrofen-induced congenital diaphragmatic hernia
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/67942 |
Resumo: | Ghrelin is a strong physiologic growth hormone secretagogue that exhibits endocrine and non-endocrine actions. In this study, ghrelin expression in humans and rats was evaluated throughout development of normal and hypoplastic lungs associated with congenital diaphragmatic hernia (CDH). Additionally, the effect of antenatal treatment with ghrelin in the nitrofen-induced CDH rat model was tested. In normal lungs, ghrelin was expressed in the primitive epithelium at early stages of development and decreased in levels of expression with gestational age. In hypoplastic lungs ghrelin was overexpressed in both human and rat CDH fetuses when compared with controls. Exogenous administration of ghrelin to nitrofen-treated dams led to an attenuation of pulmonary hypoplasia of CDH pups. Furthermore, the growth hormone, secretagogue receptor (GHSR1a), could not be amplified from human or rat fetal lungs by RT-PCR. In conclusion, of all the lungs studied so far, the fetal lung is one of the first to express ghrelin during development and might be considered a new source of circulating fetal ghrelin. Overexpression of ghrelin in hypoplastic lungs and the effect of exogenous administration of ghrelin to nitrofen-treated dams strongly suggest a role for ghrelin in mechanisms involved in attenuation of fetal lung hypoplasia, most likely through a GHSR1a-independent pathway. |
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Ghrelin expression in human and rat fetal lungs and the effect of ghrelin administration in nitrofen-induced congenital diaphragmatic herniaAnimalsFetusGestational AgeGhrelinHerbicidesHernias, Diaphragmatic, CongenitalHumansIn Situ HybridizationLungPeptide HormonesPhenyl EthersRatsReceptors, G-Protein-CoupledReceptors, GhrelinHernia, DiaphragmaticCiências Médicas::Medicina ClínicaScience & TechnologyGhrelin is a strong physiologic growth hormone secretagogue that exhibits endocrine and non-endocrine actions. In this study, ghrelin expression in humans and rats was evaluated throughout development of normal and hypoplastic lungs associated with congenital diaphragmatic hernia (CDH). Additionally, the effect of antenatal treatment with ghrelin in the nitrofen-induced CDH rat model was tested. In normal lungs, ghrelin was expressed in the primitive epithelium at early stages of development and decreased in levels of expression with gestational age. In hypoplastic lungs ghrelin was overexpressed in both human and rat CDH fetuses when compared with controls. Exogenous administration of ghrelin to nitrofen-treated dams led to an attenuation of pulmonary hypoplasia of CDH pups. Furthermore, the growth hormone, secretagogue receptor (GHSR1a), could not be amplified from human or rat fetal lungs by RT-PCR. In conclusion, of all the lungs studied so far, the fetal lung is one of the first to express ghrelin during development and might be considered a new source of circulating fetal ghrelin. Overexpression of ghrelin in hypoplastic lungs and the effect of exogenous administration of ghrelin to nitrofen-treated dams strongly suggest a role for ghrelin in mechanisms involved in attenuation of fetal lung hypoplasia, most likely through a GHSR1a-independent pathway.Springer NatureUniversidade do MinhoSantos, MartaBastos, PedroGonzaga, SilviaRoriz, José-MárioBaptista, Maria J.Silva, Cristina Isabel NogueiraRocha, Gustavo Filipe Melo AlvesHenriques-Coelho, TiagoRoncon-Albuquerque, RobertoLeite-Moreira, Adelino F.De Krijger, Ronald R.Tibboel, DickRottier, RobbertCorreia-Pinto, Jorge2006-042006-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/67942engSantos, M., Bastos, P., Gonzaga, S., Roriz, J. M., Baptista, et. al.(2006). Ghrelin expression in human and rat fetal lungs and the effect of ghrelin administration in nitrofen-induced congenital diaphragmatic hernia. Pediatric research, 59(4), 531-5370031-39981530-044710.1203/01.pdr.0000202748.66359.a916549524https://www.nature.com/articles/pr2006102info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:46:02Zoai:repositorium.sdum.uminho.pt:1822/67942Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:43:57.491498Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Ghrelin expression in human and rat fetal lungs and the effect of ghrelin administration in nitrofen-induced congenital diaphragmatic hernia |
title |
Ghrelin expression in human and rat fetal lungs and the effect of ghrelin administration in nitrofen-induced congenital diaphragmatic hernia |
spellingShingle |
Ghrelin expression in human and rat fetal lungs and the effect of ghrelin administration in nitrofen-induced congenital diaphragmatic hernia Santos, Marta Animals Fetus Gestational Age Ghrelin Herbicides Hernias, Diaphragmatic, Congenital Humans In Situ Hybridization Lung Peptide Hormones Phenyl Ethers Rats Receptors, G-Protein-Coupled Receptors, Ghrelin Hernia, Diaphragmatic Ciências Médicas::Medicina Clínica Science & Technology |
title_short |
Ghrelin expression in human and rat fetal lungs and the effect of ghrelin administration in nitrofen-induced congenital diaphragmatic hernia |
title_full |
Ghrelin expression in human and rat fetal lungs and the effect of ghrelin administration in nitrofen-induced congenital diaphragmatic hernia |
title_fullStr |
Ghrelin expression in human and rat fetal lungs and the effect of ghrelin administration in nitrofen-induced congenital diaphragmatic hernia |
title_full_unstemmed |
Ghrelin expression in human and rat fetal lungs and the effect of ghrelin administration in nitrofen-induced congenital diaphragmatic hernia |
title_sort |
Ghrelin expression in human and rat fetal lungs and the effect of ghrelin administration in nitrofen-induced congenital diaphragmatic hernia |
author |
Santos, Marta |
author_facet |
Santos, Marta Bastos, Pedro Gonzaga, Silvia Roriz, José-Mário Baptista, Maria J. Silva, Cristina Isabel Nogueira Rocha, Gustavo Filipe Melo Alves Henriques-Coelho, Tiago Roncon-Albuquerque, Roberto Leite-Moreira, Adelino F. De Krijger, Ronald R. Tibboel, Dick Rottier, Robbert Correia-Pinto, Jorge |
author_role |
author |
author2 |
Bastos, Pedro Gonzaga, Silvia Roriz, José-Mário Baptista, Maria J. Silva, Cristina Isabel Nogueira Rocha, Gustavo Filipe Melo Alves Henriques-Coelho, Tiago Roncon-Albuquerque, Roberto Leite-Moreira, Adelino F. De Krijger, Ronald R. Tibboel, Dick Rottier, Robbert Correia-Pinto, Jorge |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Santos, Marta Bastos, Pedro Gonzaga, Silvia Roriz, José-Mário Baptista, Maria J. Silva, Cristina Isabel Nogueira Rocha, Gustavo Filipe Melo Alves Henriques-Coelho, Tiago Roncon-Albuquerque, Roberto Leite-Moreira, Adelino F. De Krijger, Ronald R. Tibboel, Dick Rottier, Robbert Correia-Pinto, Jorge |
dc.subject.por.fl_str_mv |
Animals Fetus Gestational Age Ghrelin Herbicides Hernias, Diaphragmatic, Congenital Humans In Situ Hybridization Lung Peptide Hormones Phenyl Ethers Rats Receptors, G-Protein-Coupled Receptors, Ghrelin Hernia, Diaphragmatic Ciências Médicas::Medicina Clínica Science & Technology |
topic |
Animals Fetus Gestational Age Ghrelin Herbicides Hernias, Diaphragmatic, Congenital Humans In Situ Hybridization Lung Peptide Hormones Phenyl Ethers Rats Receptors, G-Protein-Coupled Receptors, Ghrelin Hernia, Diaphragmatic Ciências Médicas::Medicina Clínica Science & Technology |
description |
Ghrelin is a strong physiologic growth hormone secretagogue that exhibits endocrine and non-endocrine actions. In this study, ghrelin expression in humans and rats was evaluated throughout development of normal and hypoplastic lungs associated with congenital diaphragmatic hernia (CDH). Additionally, the effect of antenatal treatment with ghrelin in the nitrofen-induced CDH rat model was tested. In normal lungs, ghrelin was expressed in the primitive epithelium at early stages of development and decreased in levels of expression with gestational age. In hypoplastic lungs ghrelin was overexpressed in both human and rat CDH fetuses when compared with controls. Exogenous administration of ghrelin to nitrofen-treated dams led to an attenuation of pulmonary hypoplasia of CDH pups. Furthermore, the growth hormone, secretagogue receptor (GHSR1a), could not be amplified from human or rat fetal lungs by RT-PCR. In conclusion, of all the lungs studied so far, the fetal lung is one of the first to express ghrelin during development and might be considered a new source of circulating fetal ghrelin. Overexpression of ghrelin in hypoplastic lungs and the effect of exogenous administration of ghrelin to nitrofen-treated dams strongly suggest a role for ghrelin in mechanisms involved in attenuation of fetal lung hypoplasia, most likely through a GHSR1a-independent pathway. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-04 2006-04-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/67942 |
url |
http://hdl.handle.net/1822/67942 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Santos, M., Bastos, P., Gonzaga, S., Roriz, J. M., Baptista, et. al.(2006). Ghrelin expression in human and rat fetal lungs and the effect of ghrelin administration in nitrofen-induced congenital diaphragmatic hernia. Pediatric research, 59(4), 531-537 0031-3998 1530-0447 10.1203/01.pdr.0000202748.66359.a9 16549524 https://www.nature.com/articles/pr2006102 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer Nature |
publisher.none.fl_str_mv |
Springer Nature |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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