Comparison of culture- and quantitative PCR-Based indicators of antibiotic resistance in wastewater, recycled water, and tap water
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/28705 |
Resumo: | Standardized methods are needed to support monitoring of antibiotic resistance in environmental samples. Culture-based methods target species of human-health relevance, while the direct quantification of antibiotic resistance genes (ARGs) measures the antibiotic resistance potential in the microbial community. This study compared measurements of tetracycline-, sulphonamide-, and cefotaxime-resistant presumptive total and fecal coliforms and presumptive enterococci versus a suite of ARGs quantified by quantitative polymerase chain reaction (qPCR) across waste-, recycled-, tap-, and freshwater. Cross-laboratory comparison of results involved measurements on samples collected and analysed in the US and Portugal. The same DNA extracts analysed in the US and Portugal produced comparable qPCR results (variation <28%), except for blaOXA-1 gene (0%–57%). Presumptive total and fecal coliforms and cefotaxime-resistant total coliforms strongly correlated with blaCTX-M and intI1 (0.725 ≤ R2 ≤ 0.762; p < 0.0001). Further, presumptive total and fecal coliforms correlated with the Escherichia coli-specific biomarkers, gadAB, and uidA, suggesting that both methods captured fecal-sourced bacteria. The genes encoding resistance to sulphonamides (sul1 and sul2) were the most abundant, followed by genes encoding resistance to tetracyclines (tet(A) and tet(O)) and β-lactams (blaOXA-1 and, blaCTX-M), which was in agreement with the culture-based enumerations. The findings can help inform future application of methods being considered for international antibiotic resistance surveillance in the environment. |
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Comparison of culture- and quantitative PCR-Based indicators of antibiotic resistance in wastewater, recycled water, and tap waterAntibiotic resistance monitoringAntibiotic resistant coliformsWater qualityStandardized methods are needed to support monitoring of antibiotic resistance in environmental samples. Culture-based methods target species of human-health relevance, while the direct quantification of antibiotic resistance genes (ARGs) measures the antibiotic resistance potential in the microbial community. This study compared measurements of tetracycline-, sulphonamide-, and cefotaxime-resistant presumptive total and fecal coliforms and presumptive enterococci versus a suite of ARGs quantified by quantitative polymerase chain reaction (qPCR) across waste-, recycled-, tap-, and freshwater. Cross-laboratory comparison of results involved measurements on samples collected and analysed in the US and Portugal. The same DNA extracts analysed in the US and Portugal produced comparable qPCR results (variation <28%), except for blaOXA-1 gene (0%–57%). Presumptive total and fecal coliforms and cefotaxime-resistant total coliforms strongly correlated with blaCTX-M and intI1 (0.725 ≤ R2 ≤ 0.762; p < 0.0001). Further, presumptive total and fecal coliforms correlated with the Escherichia coli-specific biomarkers, gadAB, and uidA, suggesting that both methods captured fecal-sourced bacteria. The genes encoding resistance to sulphonamides (sul1 and sul2) were the most abundant, followed by genes encoding resistance to tetracyclines (tet(A) and tet(O)) and β-lactams (blaOXA-1 and, blaCTX-M), which was in agreement with the culture-based enumerations. The findings can help inform future application of methods being considered for international antibiotic resistance surveillance in the environment.MDPIVeritati - Repositório Institucional da Universidade Católica PortuguesaRocha, JaquelineFernandes, TelmaRiquelme, Maria V.Zhu, NiPruden, AmyManaia, Célia M.2019-11-12T19:30:56Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/28705engRocha, J., Fernandes, T., Riquelme, M. V., Zhu, N., Pruden, A., & Manaia, C. M. (2019). Comparison of culture- and quantitative PCR-Based indicators of antibiotic resistance in wastewater, recycled water, and tap water. International Journal of Environmental Research and Public Health, 16(21), 4217. https://doi.org/10.3390/ijerph1621421710.3390/ijerph162142171660-460185074343266PMC686266431671709000498842000163info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-03T01:42:04Zoai:repositorio.ucp.pt:10400.14/28705Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:23:02.632286Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Comparison of culture- and quantitative PCR-Based indicators of antibiotic resistance in wastewater, recycled water, and tap water |
title |
Comparison of culture- and quantitative PCR-Based indicators of antibiotic resistance in wastewater, recycled water, and tap water |
spellingShingle |
Comparison of culture- and quantitative PCR-Based indicators of antibiotic resistance in wastewater, recycled water, and tap water Rocha, Jaqueline Antibiotic resistance monitoring Antibiotic resistant coliforms Water quality |
title_short |
Comparison of culture- and quantitative PCR-Based indicators of antibiotic resistance in wastewater, recycled water, and tap water |
title_full |
Comparison of culture- and quantitative PCR-Based indicators of antibiotic resistance in wastewater, recycled water, and tap water |
title_fullStr |
Comparison of culture- and quantitative PCR-Based indicators of antibiotic resistance in wastewater, recycled water, and tap water |
title_full_unstemmed |
Comparison of culture- and quantitative PCR-Based indicators of antibiotic resistance in wastewater, recycled water, and tap water |
title_sort |
Comparison of culture- and quantitative PCR-Based indicators of antibiotic resistance in wastewater, recycled water, and tap water |
author |
Rocha, Jaqueline |
author_facet |
Rocha, Jaqueline Fernandes, Telma Riquelme, Maria V. Zhu, Ni Pruden, Amy Manaia, Célia M. |
author_role |
author |
author2 |
Fernandes, Telma Riquelme, Maria V. Zhu, Ni Pruden, Amy Manaia, Célia M. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Rocha, Jaqueline Fernandes, Telma Riquelme, Maria V. Zhu, Ni Pruden, Amy Manaia, Célia M. |
dc.subject.por.fl_str_mv |
Antibiotic resistance monitoring Antibiotic resistant coliforms Water quality |
topic |
Antibiotic resistance monitoring Antibiotic resistant coliforms Water quality |
description |
Standardized methods are needed to support monitoring of antibiotic resistance in environmental samples. Culture-based methods target species of human-health relevance, while the direct quantification of antibiotic resistance genes (ARGs) measures the antibiotic resistance potential in the microbial community. This study compared measurements of tetracycline-, sulphonamide-, and cefotaxime-resistant presumptive total and fecal coliforms and presumptive enterococci versus a suite of ARGs quantified by quantitative polymerase chain reaction (qPCR) across waste-, recycled-, tap-, and freshwater. Cross-laboratory comparison of results involved measurements on samples collected and analysed in the US and Portugal. The same DNA extracts analysed in the US and Portugal produced comparable qPCR results (variation <28%), except for blaOXA-1 gene (0%–57%). Presumptive total and fecal coliforms and cefotaxime-resistant total coliforms strongly correlated with blaCTX-M and intI1 (0.725 ≤ R2 ≤ 0.762; p < 0.0001). Further, presumptive total and fecal coliforms correlated with the Escherichia coli-specific biomarkers, gadAB, and uidA, suggesting that both methods captured fecal-sourced bacteria. The genes encoding resistance to sulphonamides (sul1 and sul2) were the most abundant, followed by genes encoding resistance to tetracyclines (tet(A) and tet(O)) and β-lactams (blaOXA-1 and, blaCTX-M), which was in agreement with the culture-based enumerations. The findings can help inform future application of methods being considered for international antibiotic resistance surveillance in the environment. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11-12T19:30:56Z 2019 2019-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/28705 |
url |
http://hdl.handle.net/10400.14/28705 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Rocha, J., Fernandes, T., Riquelme, M. V., Zhu, N., Pruden, A., & Manaia, C. M. (2019). Comparison of culture- and quantitative PCR-Based indicators of antibiotic resistance in wastewater, recycled water, and tap water. International Journal of Environmental Research and Public Health, 16(21), 4217. https://doi.org/10.3390/ijerph16214217 10.3390/ijerph16214217 1660-4601 85074343266 PMC6862664 31671709 000498842000163 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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