Genetic Diversity of norA, Coding for a Main Efflux Pump of Staphylococcus aureus

Detalhes bibliográficos
Autor(a) principal: Santos Costa, Sofia
Data de Publicação: 2019
Outros Autores: Sobkowiak, Benjamin, Parreira, Ricardo, Edgeworth, Jonathan D., Viveiros, Miguel, Clark, Taane G., Couto, Isabel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/116697
Resumo: Funding Information: This work was partially supported by Fundação para a Ciência e a Tecnologia (FCT, Portugal), through funds to GHTM – UID/Multi/04413/2013. SC was supported by grant SFRH/BPD/97508/2013 from FCT, Portugal. TC was funded by the Medical Research Council United Kingdom (Grant Nos. MR/K000551/1, MR/M01360X/1, MR/N010469/1, and MR/R020973/1) and BBSRC United Kingdom (BB/R013063/1). BS was funded by the Medical Research Council United Kingdom (Grant No. MR/N010469/1). Publisher Copyright: © 2007 - 2019 Frontiers Media S.A. All Rights Reserved.
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spelling Genetic Diversity of norA, Coding for a Main Efflux Pump of Staphylococcus aureusStaphylococcus aureusnorAAllelesVariabilityEffluxBiochemistry, Genetics and Molecular Biology (miscellaneous)MicrobiologyInfectious DiseasesSDG 3 - Good Health and Well-beingFunding Information: This work was partially supported by Fundação para a Ciência e a Tecnologia (FCT, Portugal), through funds to GHTM – UID/Multi/04413/2013. SC was supported by grant SFRH/BPD/97508/2013 from FCT, Portugal. TC was funded by the Medical Research Council United Kingdom (Grant Nos. MR/K000551/1, MR/M01360X/1, MR/N010469/1, and MR/R020973/1) and BBSRC United Kingdom (BB/R013063/1). BS was funded by the Medical Research Council United Kingdom (Grant No. MR/N010469/1). Publisher Copyright: © 2007 - 2019 Frontiers Media S.A. All Rights Reserved.NorA is the best studied efflux system of Staphylococcus aureus and therefore frequently used as a model for investigating efflux-mediated resistance in this pathogen. NorA activity is associated with resistance to fluoroquinolones, several antiseptics and disinfectants and several reports have pointed out the role of efflux systems, including NorA, as a first-line response to antimicrobials in S. aureus. Genetic diversity studies of the gene norA have described three alleles; norAI, norAII and norAIII. However, the epidemiology of these alleles and their impact on NorA activity remains unclear. Additionally, increasing studies do not account for norA variability when establishing relations between resistance phenotypes and norA presence or reported absence, which actually corresponds, as we now demonstrate, to different norA alleles. In the present study we assessed the variability of the norA gene present in the genome of over 1,000 S. aureus isolates, corresponding to 112 S. aureus strains with whole genome sequences publicly available; 917 MRSA strains sourced from a London-based study and nine MRSA isolates collected in a major Hospital in Lisbon, Portugal. Our analyses show that norA is part of the core genome of S. aureus. It also suggests that occurrence of norA variants reflects the population structure of this major pathogen. Overall, this work highlights the ubiquitous nature of norA in S. aureus which must be taken into account when studying the role played by this important determinant on S. aureus resistance to antimicrobials.TB, HIV and opportunistic diseases and pathogens (THOP)Global Health and Tropical Medicine (GHTM)Instituto de Higiene e Medicina Tropical (IHMT)Vector borne diseases and pathogens (VBD)RUNSantos Costa, SofiaSobkowiak, BenjaminParreira, RicardoEdgeworth, Jonathan D.Viveiros, MiguelClark, Taane G.Couto, Isabel2021-05-02T22:40:27Z2019-01-092019-01-09T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://hdl.handle.net/10362/116697eng1664-8021PURE: 11385065https://doi.org/10.3389/fgene.2018.00710info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:59:22Zoai:run.unl.pt:10362/116697Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:43:12.738842Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genetic Diversity of norA, Coding for a Main Efflux Pump of Staphylococcus aureus
title Genetic Diversity of norA, Coding for a Main Efflux Pump of Staphylococcus aureus
spellingShingle Genetic Diversity of norA, Coding for a Main Efflux Pump of Staphylococcus aureus
Santos Costa, Sofia
Staphylococcus aureus
norA
Alleles
Variability
Efflux
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Microbiology
Infectious Diseases
SDG 3 - Good Health and Well-being
title_short Genetic Diversity of norA, Coding for a Main Efflux Pump of Staphylococcus aureus
title_full Genetic Diversity of norA, Coding for a Main Efflux Pump of Staphylococcus aureus
title_fullStr Genetic Diversity of norA, Coding for a Main Efflux Pump of Staphylococcus aureus
title_full_unstemmed Genetic Diversity of norA, Coding for a Main Efflux Pump of Staphylococcus aureus
title_sort Genetic Diversity of norA, Coding for a Main Efflux Pump of Staphylococcus aureus
author Santos Costa, Sofia
author_facet Santos Costa, Sofia
Sobkowiak, Benjamin
Parreira, Ricardo
Edgeworth, Jonathan D.
Viveiros, Miguel
Clark, Taane G.
Couto, Isabel
author_role author
author2 Sobkowiak, Benjamin
Parreira, Ricardo
Edgeworth, Jonathan D.
Viveiros, Miguel
Clark, Taane G.
Couto, Isabel
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv TB, HIV and opportunistic diseases and pathogens (THOP)
Global Health and Tropical Medicine (GHTM)
Instituto de Higiene e Medicina Tropical (IHMT)
Vector borne diseases and pathogens (VBD)
RUN
dc.contributor.author.fl_str_mv Santos Costa, Sofia
Sobkowiak, Benjamin
Parreira, Ricardo
Edgeworth, Jonathan D.
Viveiros, Miguel
Clark, Taane G.
Couto, Isabel
dc.subject.por.fl_str_mv Staphylococcus aureus
norA
Alleles
Variability
Efflux
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Microbiology
Infectious Diseases
SDG 3 - Good Health and Well-being
topic Staphylococcus aureus
norA
Alleles
Variability
Efflux
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Microbiology
Infectious Diseases
SDG 3 - Good Health and Well-being
description Funding Information: This work was partially supported by Fundação para a Ciência e a Tecnologia (FCT, Portugal), through funds to GHTM – UID/Multi/04413/2013. SC was supported by grant SFRH/BPD/97508/2013 from FCT, Portugal. TC was funded by the Medical Research Council United Kingdom (Grant Nos. MR/K000551/1, MR/M01360X/1, MR/N010469/1, and MR/R020973/1) and BBSRC United Kingdom (BB/R013063/1). BS was funded by the Medical Research Council United Kingdom (Grant No. MR/N010469/1). Publisher Copyright: © 2007 - 2019 Frontiers Media S.A. All Rights Reserved.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-09
2019-01-09T00:00:00Z
2021-05-02T22:40:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/116697
url http://hdl.handle.net/10362/116697
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1664-8021
PURE: 11385065
https://doi.org/10.3389/fgene.2018.00710
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11
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dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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