Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/54421 |
Resumo: | Glycerol is a main co-product of biodiesel production. Crude glycerol may serve as a cheap and attractive substrate in biotechnological applications, e.g. for the production of valuable chemicals or as an electron donor for reduction processes. In this work, sulfate reduction with glycerol was studied at neutral and acidic pH using bioreactor sludge samples and Tinto River sediments as a source of inoculum, respectively. Communities of sulfate-reducing bacteria (SRB) and fermentative bacteria were co-enriched at both pH values. Molecular analyses revealed that sequences belonging to Desulfomicrobium genus were dominant in the cultures enriched at pH 7, while Desulfosporosinus sequences dominated in the culture enriched at pH 4. Glycerol conversion was coupled to sulfate reduction, but the substrate was incompletely oxidized to acetate in the neutrophilic enrichments, and acetate, lactate, and 1,3-propanediol under low pH conditions. Two strains belonging to Desulfomicrobium and Proteiniphilum genera were isolated from the neutrophilic enrichments, but the first isolate was not able to use glycerol, which suggests a syntrophic relationship between glycerol-degrading fermentative bacteria and SRB. A Clostridium strain able to grow with glycerol was isolated from the low pH enrichment. Our data indicate that glycerol promotes the growth of sulfate-reducing communities to form sulfide, which can be used to precipitate and recover heavy metals. |
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Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pHFermentative bacteriaGlycerolMetal recoverySulfate-reducing bacteriaSyntrophyScience & TechnologyGlycerol is a main co-product of biodiesel production. Crude glycerol may serve as a cheap and attractive substrate in biotechnological applications, e.g. for the production of valuable chemicals or as an electron donor for reduction processes. In this work, sulfate reduction with glycerol was studied at neutral and acidic pH using bioreactor sludge samples and Tinto River sediments as a source of inoculum, respectively. Communities of sulfate-reducing bacteria (SRB) and fermentative bacteria were co-enriched at both pH values. Molecular analyses revealed that sequences belonging to Desulfomicrobium genus were dominant in the cultures enriched at pH 7, while Desulfosporosinus sequences dominated in the culture enriched at pH 4. Glycerol conversion was coupled to sulfate reduction, but the substrate was incompletely oxidized to acetate in the neutrophilic enrichments, and acetate, lactate, and 1,3-propanediol under low pH conditions. Two strains belonging to Desulfomicrobium and Proteiniphilum genera were isolated from the neutrophilic enrichments, but the first isolate was not able to use glycerol, which suggests a syntrophic relationship between glycerol-degrading fermentative bacteria and SRB. A Clostridium strain able to grow with glycerol was isolated from the low pH enrichment. Our data indicate that glycerol promotes the growth of sulfate-reducing communities to form sulfide, which can be used to precipitate and recover heavy metals.The authors thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), the Spanish Ministerio de Ciencia e Innovación (grant CTM2009-10521), the European Research Council (project 323009) and the graduate school WIMEK SENSE for support. The research was part of the PhD program of the Northeast Brazil Biotechnology Network (RENORBIO).info:eu-repo/semantics/publishedVersionMicrobiology Research FoundationUniversidade do MinhoSantos, Sidnei C.Liebensteiner, Martin G.van Gelder, Antonie H.Dimitrov, Mauricio R.Almeida, Paulo F.Quintella, Cristina M.Stams, Alfons Johannes MariaSánchez-Andrea, Irene20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/54421engSantos, Sidnei C.; Liebensteiner, Martin G.; van Gelder, Antonie H.; Dimitrov, Mauricio R.; Almeida, Paulo F.; Quintella, Cristina M.; Stams, A. J. M.; Sánchez-Andrea, Irene, Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH. Journal of General and Applied Microbiology, 64(1), 1-8, 20180022-12601349-803710.2323/jgam.2017.02.00929187682https://www.jstage.jst.go.jp/browse/jgam/-char/eninfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:09:04Zoai:repositorium.sdum.uminho.pt:1822/54421Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:00:25.561642Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH |
title |
Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH |
spellingShingle |
Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH Santos, Sidnei C. Fermentative bacteria Glycerol Metal recovery Sulfate-reducing bacteria Syntrophy Science & Technology |
title_short |
Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH |
title_full |
Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH |
title_fullStr |
Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH |
title_full_unstemmed |
Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH |
title_sort |
Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH |
author |
Santos, Sidnei C. |
author_facet |
Santos, Sidnei C. Liebensteiner, Martin G. van Gelder, Antonie H. Dimitrov, Mauricio R. Almeida, Paulo F. Quintella, Cristina M. Stams, Alfons Johannes Maria Sánchez-Andrea, Irene |
author_role |
author |
author2 |
Liebensteiner, Martin G. van Gelder, Antonie H. Dimitrov, Mauricio R. Almeida, Paulo F. Quintella, Cristina M. Stams, Alfons Johannes Maria Sánchez-Andrea, Irene |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Santos, Sidnei C. Liebensteiner, Martin G. van Gelder, Antonie H. Dimitrov, Mauricio R. Almeida, Paulo F. Quintella, Cristina M. Stams, Alfons Johannes Maria Sánchez-Andrea, Irene |
dc.subject.por.fl_str_mv |
Fermentative bacteria Glycerol Metal recovery Sulfate-reducing bacteria Syntrophy Science & Technology |
topic |
Fermentative bacteria Glycerol Metal recovery Sulfate-reducing bacteria Syntrophy Science & Technology |
description |
Glycerol is a main co-product of biodiesel production. Crude glycerol may serve as a cheap and attractive substrate in biotechnological applications, e.g. for the production of valuable chemicals or as an electron donor for reduction processes. In this work, sulfate reduction with glycerol was studied at neutral and acidic pH using bioreactor sludge samples and Tinto River sediments as a source of inoculum, respectively. Communities of sulfate-reducing bacteria (SRB) and fermentative bacteria were co-enriched at both pH values. Molecular analyses revealed that sequences belonging to Desulfomicrobium genus were dominant in the cultures enriched at pH 7, while Desulfosporosinus sequences dominated in the culture enriched at pH 4. Glycerol conversion was coupled to sulfate reduction, but the substrate was incompletely oxidized to acetate in the neutrophilic enrichments, and acetate, lactate, and 1,3-propanediol under low pH conditions. Two strains belonging to Desulfomicrobium and Proteiniphilum genera were isolated from the neutrophilic enrichments, but the first isolate was not able to use glycerol, which suggests a syntrophic relationship between glycerol-degrading fermentative bacteria and SRB. A Clostridium strain able to grow with glycerol was isolated from the low pH enrichment. Our data indicate that glycerol promotes the growth of sulfate-reducing communities to form sulfide, which can be used to precipitate and recover heavy metals. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 2018-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/54421 |
url |
http://hdl.handle.net/1822/54421 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Santos, Sidnei C.; Liebensteiner, Martin G.; van Gelder, Antonie H.; Dimitrov, Mauricio R.; Almeida, Paulo F.; Quintella, Cristina M.; Stams, A. J. M.; Sánchez-Andrea, Irene, Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH. Journal of General and Applied Microbiology, 64(1), 1-8, 2018 0022-1260 1349-8037 10.2323/jgam.2017.02.009 29187682 https://www.jstage.jst.go.jp/browse/jgam/-char/en |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Microbiology Research Foundation |
publisher.none.fl_str_mv |
Microbiology Research Foundation |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132399561342976 |