Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/8820 |
Resumo: | Magnetized polysiloxane coated with polyaniline (mPOS–PANI) was used as a support for β-galactosidase immobilization via glutaraldehyde. The galactooligosaccharides (GOS) production by this derivative was investigated under different initial lactose concentrations (5–50%) and temperatures (30–60 °C). The initial lactose concentration in the reaction media affected the total amounts of produced GOS and their time course production was described as a “bell-shaped” curve as a result of the balance between transgalactosylation and hydrolysis. No significative difference was observed for the free and immobilized enzymes. The reaction rates for lactose hydrolysis and GOS formation increased with increasing temperature from 30 °C to 60 °C, but GOS production at all lactose conversion levels was almost unchanged with changing temperature. The mPOS–PANI matrix was also characterized by scanning electronic microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), vibrating sample magnetometry (VSM), thermomagnetization, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). |
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7160 |
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Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particlesβ-galactosidaseMagnetic supportPolysiloxanePolyanilineGalactooligosaccharidesCovalent immobilizationbeta-galactosidaseScience & TechnologyMagnetized polysiloxane coated with polyaniline (mPOS–PANI) was used as a support for β-galactosidase immobilization via glutaraldehyde. The galactooligosaccharides (GOS) production by this derivative was investigated under different initial lactose concentrations (5–50%) and temperatures (30–60 °C). The initial lactose concentration in the reaction media affected the total amounts of produced GOS and their time course production was described as a “bell-shaped” curve as a result of the balance between transgalactosylation and hydrolysis. No significative difference was observed for the free and immobilized enzymes. The reaction rates for lactose hydrolysis and GOS formation increased with increasing temperature from 30 °C to 60 °C, but GOS production at all lactose conversion levels was almost unchanged with changing temperature. The mPOS–PANI matrix was also characterized by scanning electronic microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), vibrating sample magnetometry (VSM), thermomagnetization, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA).European Union Programme of High Level Scholarships for Latin America - Programme Alban ((Scholarship No. E05D057787BR)Brazilian National Research Council (CNPq)Elsevier Ltd.Universidade do MinhoNeri, D. F. M.Balcão, V. M.Dourado, FernandoOliveira, J. M.Carvalho Junior, L. B.Teixeira, J. A.20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/8820engNERI, David F. M. [et al.] - Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles. “Reactive and Functional Polymers” [Em linha]. 69:4 (Apr. 2009) 246-251. [Consult. 25 Mar. 2009]. Disponível em WWW:<URL: http://www.sciencedirect.com/science?_ob= ArticleURL&_udi=B6TY0-4VDS8FN-1&_user= 2459786&_rdoc=1&_fmt=&_orig=search&_sort= d&view=c&_acct=C000057396&_version=1&_urlVersion=0&_userid=2459786&md5= c69b9a88ad4302f0fc6a009cb9b5e402>. ISSN 1381-5148.1381-514810.1016/j.reactfunctpolym.2009.01.002http://www.sciencedirect.com/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:25:24Zoai:repositorium.sdum.uminho.pt:1822/8820Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:19:38.499902Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles |
title |
Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles |
spellingShingle |
Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles Neri, D. F. M. β-galactosidase Magnetic support Polysiloxane Polyaniline Galactooligosaccharides Covalent immobilization beta-galactosidase Science & Technology |
title_short |
Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles |
title_full |
Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles |
title_fullStr |
Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles |
title_full_unstemmed |
Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles |
title_sort |
Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles |
author |
Neri, D. F. M. |
author_facet |
Neri, D. F. M. Balcão, V. M. Dourado, Fernando Oliveira, J. M. Carvalho Junior, L. B. Teixeira, J. A. |
author_role |
author |
author2 |
Balcão, V. M. Dourado, Fernando Oliveira, J. M. Carvalho Junior, L. B. Teixeira, J. A. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Neri, D. F. M. Balcão, V. M. Dourado, Fernando Oliveira, J. M. Carvalho Junior, L. B. Teixeira, J. A. |
dc.subject.por.fl_str_mv |
β-galactosidase Magnetic support Polysiloxane Polyaniline Galactooligosaccharides Covalent immobilization beta-galactosidase Science & Technology |
topic |
β-galactosidase Magnetic support Polysiloxane Polyaniline Galactooligosaccharides Covalent immobilization beta-galactosidase Science & Technology |
description |
Magnetized polysiloxane coated with polyaniline (mPOS–PANI) was used as a support for β-galactosidase immobilization via glutaraldehyde. The galactooligosaccharides (GOS) production by this derivative was investigated under different initial lactose concentrations (5–50%) and temperatures (30–60 °C). The initial lactose concentration in the reaction media affected the total amounts of produced GOS and their time course production was described as a “bell-shaped” curve as a result of the balance between transgalactosylation and hydrolysis. No significative difference was observed for the free and immobilized enzymes. The reaction rates for lactose hydrolysis and GOS formation increased with increasing temperature from 30 °C to 60 °C, but GOS production at all lactose conversion levels was almost unchanged with changing temperature. The mPOS–PANI matrix was also characterized by scanning electronic microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), vibrating sample magnetometry (VSM), thermomagnetization, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009 2009-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/8820 |
url |
http://hdl.handle.net/1822/8820 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
NERI, David F. M. [et al.] - Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles. “Reactive and Functional Polymers” [Em linha]. 69:4 (Apr. 2009) 246-251. [Consult. 25 Mar. 2009]. Disponível em WWW:<URL: http://www.sciencedirect.com/science?_ob= ArticleURL&_udi=B6TY0-4VDS8FN-1&_user= 2459786&_rdoc=1&_fmt=&_orig=search&_sort= d&view=c&_acct=C000057396&_version=1&_urlVersion=0&_userid=2459786&md5= c69b9a88ad4302f0fc6a009cb9b5e402>. ISSN 1381-5148. 1381-5148 10.1016/j.reactfunctpolym.2009.01.002 http://www.sciencedirect.com/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Ltd. |
publisher.none.fl_str_mv |
Elsevier Ltd. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132656074489856 |