Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles

Detalhes bibliográficos
Autor(a) principal: Neri, D. F. M.
Data de Publicação: 2009
Outros Autores: Balcão, V. M., Dourado, Fernando, Oliveira, J. M., Carvalho Junior, L. B., Teixeira, J. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/8820
Resumo: Magnetized polysiloxane coated with polyaniline (mPOS–PANI) was used as a support for β-galactosidase immobilization via glutaraldehyde. The galactooligosaccharides (GOS) production by this derivative was investigated under different initial lactose concentrations (5–50%) and temperatures (30–60 °C). The initial lactose concentration in the reaction media affected the total amounts of produced GOS and their time course production was described as a “bell-shaped” curve as a result of the balance between transgalactosylation and hydrolysis. No significative difference was observed for the free and immobilized enzymes. The reaction rates for lactose hydrolysis and GOS formation increased with increasing temperature from 30 °C to 60 °C, but GOS production at all lactose conversion levels was almost unchanged with changing temperature. The mPOS–PANI matrix was also characterized by scanning electronic microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), vibrating sample magnetometry (VSM), thermomagnetization, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA).
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spelling Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particlesβ-galactosidaseMagnetic supportPolysiloxanePolyanilineGalactooligosaccharidesCovalent immobilizationbeta-galactosidaseScience & TechnologyMagnetized polysiloxane coated with polyaniline (mPOS–PANI) was used as a support for β-galactosidase immobilization via glutaraldehyde. The galactooligosaccharides (GOS) production by this derivative was investigated under different initial lactose concentrations (5–50%) and temperatures (30–60 °C). The initial lactose concentration in the reaction media affected the total amounts of produced GOS and their time course production was described as a “bell-shaped” curve as a result of the balance between transgalactosylation and hydrolysis. No significative difference was observed for the free and immobilized enzymes. The reaction rates for lactose hydrolysis and GOS formation increased with increasing temperature from 30 °C to 60 °C, but GOS production at all lactose conversion levels was almost unchanged with changing temperature. The mPOS–PANI matrix was also characterized by scanning electronic microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), vibrating sample magnetometry (VSM), thermomagnetization, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA).European Union Programme of High Level Scholarships for Latin America - Programme Alban ((Scholarship No. E05D057787BR)Brazilian National Research Council (CNPq)Elsevier Ltd.Universidade do MinhoNeri, D. F. M.Balcão, V. M.Dourado, FernandoOliveira, J. M.Carvalho Junior, L. B.Teixeira, J. A.20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/8820engNERI, David F. M. [et al.] - Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles. “Reactive and Functional Polymers” [Em linha]. 69:4 (Apr. 2009) 246-251. [Consult. 25 Mar. 2009]. Disponível em WWW:<URL: http://www.sciencedirect.com/science?_ob= ArticleURL&_udi=B6TY0-4VDS8FN-1&_user= 2459786&_rdoc=1&_fmt=&_orig=search&_sort= d&view=c&_acct=C000057396&_version=1&_urlVersion=0&_userid=2459786&md5= c69b9a88ad4302f0fc6a009cb9b5e402>. ISSN 1381-5148.1381-514810.1016/j.reactfunctpolym.2009.01.002http://www.sciencedirect.com/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:25:24Zoai:repositorium.sdum.uminho.pt:1822/8820Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:19:38.499902Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles
title Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles
spellingShingle Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles
Neri, D. F. M.
β-galactosidase
Magnetic support
Polysiloxane
Polyaniline
Galactooligosaccharides
Covalent immobilization
beta-galactosidase
Science & Technology
title_short Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles
title_full Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles
title_fullStr Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles
title_full_unstemmed Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles
title_sort Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles
author Neri, D. F. M.
author_facet Neri, D. F. M.
Balcão, V. M.
Dourado, Fernando
Oliveira, J. M.
Carvalho Junior, L. B.
Teixeira, J. A.
author_role author
author2 Balcão, V. M.
Dourado, Fernando
Oliveira, J. M.
Carvalho Junior, L. B.
Teixeira, J. A.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Neri, D. F. M.
Balcão, V. M.
Dourado, Fernando
Oliveira, J. M.
Carvalho Junior, L. B.
Teixeira, J. A.
dc.subject.por.fl_str_mv β-galactosidase
Magnetic support
Polysiloxane
Polyaniline
Galactooligosaccharides
Covalent immobilization
beta-galactosidase
Science & Technology
topic β-galactosidase
Magnetic support
Polysiloxane
Polyaniline
Galactooligosaccharides
Covalent immobilization
beta-galactosidase
Science & Technology
description Magnetized polysiloxane coated with polyaniline (mPOS–PANI) was used as a support for β-galactosidase immobilization via glutaraldehyde. The galactooligosaccharides (GOS) production by this derivative was investigated under different initial lactose concentrations (5–50%) and temperatures (30–60 °C). The initial lactose concentration in the reaction media affected the total amounts of produced GOS and their time course production was described as a “bell-shaped” curve as a result of the balance between transgalactosylation and hydrolysis. No significative difference was observed for the free and immobilized enzymes. The reaction rates for lactose hydrolysis and GOS formation increased with increasing temperature from 30 °C to 60 °C, but GOS production at all lactose conversion levels was almost unchanged with changing temperature. The mPOS–PANI matrix was also characterized by scanning electronic microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), vibrating sample magnetometry (VSM), thermomagnetization, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA).
publishDate 2009
dc.date.none.fl_str_mv 2009
2009-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/8820
url http://hdl.handle.net/1822/8820
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv NERI, David F. M. [et al.] - Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles. “Reactive and Functional Polymers” [Em linha]. 69:4 (Apr. 2009) 246-251. [Consult. 25 Mar. 2009]. Disponível em WWW:<URL: http://www.sciencedirect.com/science?_ob= ArticleURL&_udi=B6TY0-4VDS8FN-1&_user= 2459786&_rdoc=1&_fmt=&_orig=search&_sort= d&view=c&_acct=C000057396&_version=1&_urlVersion=0&_userid=2459786&md5= c69b9a88ad4302f0fc6a009cb9b5e402>. ISSN 1381-5148.
1381-5148
10.1016/j.reactfunctpolym.2009.01.002
http://www.sciencedirect.com/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier Ltd.
publisher.none.fl_str_mv Elsevier Ltd.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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