Assessing the performance of analytical methods for propolis – A collaborative trial by the international honey commission
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/28305 |
Resumo: | Propolis is a resinous beehive product with extraordinary bioactivity and chemical richness, linked with the botanical sources of the resin. The potential of this product keeps captivating the scientific community, conducting to continuous and growing research on plant sources, composition, or applications in agriculture, cosmetics, pharmacy, odontology, etc. In all cases, the quality assessment is a requirement and relies on methods to extract the bioactive substances from the raw propolis and quantify different components. Unfortunately, besides the absence of international quality requirements, there is also a lack of standardized analytical procedures, despite the presence of several methodologies with unknown reliability, often not comparable. To overcome the current status, the International Honey Commission established an inter-laboratory study, with propolis samples from around the globe, to harmonize analytical methods and evaluate their accuracy. A common set of protocols was matched between twelve laboratories from nine countries, for quantification of ash, wax, and balsamic content in raw propolis, and spectrophotometric evaluation of total phenolics, flavone/flavonol, and flavanone/ dihydroflavonol in the extract. A total of 3428 results (97% valid data), were used to assess the methods’ accuracy following ISO-5725 guidelines. The within-laboratory precision, revealed good agreement levels for the majority of the methods, with relative variance below 5%. As expected, the between-laboratory variance increased, but, with exception of the flavanone method that revealed a clear lack of consistency, all the others maintained acceptable variability levels, below 30%. Because the performance of ultrasounds procedures was low, they cannot be recommended until further improvements are made. |
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Assessing the performance of analytical methods for propolis – A collaborative trial by the international honey commissionPropolisCollaborative studyMethods harmonizationStandard methodsInternational Honey CommissionPropolis is a resinous beehive product with extraordinary bioactivity and chemical richness, linked with the botanical sources of the resin. The potential of this product keeps captivating the scientific community, conducting to continuous and growing research on plant sources, composition, or applications in agriculture, cosmetics, pharmacy, odontology, etc. In all cases, the quality assessment is a requirement and relies on methods to extract the bioactive substances from the raw propolis and quantify different components. Unfortunately, besides the absence of international quality requirements, there is also a lack of standardized analytical procedures, despite the presence of several methodologies with unknown reliability, often not comparable. To overcome the current status, the International Honey Commission established an inter-laboratory study, with propolis samples from around the globe, to harmonize analytical methods and evaluate their accuracy. A common set of protocols was matched between twelve laboratories from nine countries, for quantification of ash, wax, and balsamic content in raw propolis, and spectrophotometric evaluation of total phenolics, flavone/flavonol, and flavanone/ dihydroflavonol in the extract. A total of 3428 results (97% valid data), were used to assess the methods’ accuracy following ISO-5725 guidelines. The within-laboratory precision, revealed good agreement levels for the majority of the methods, with relative variance below 5%. As expected, the between-laboratory variance increased, but, with exception of the flavanone method that revealed a clear lack of consistency, all the others maintained acceptable variability levels, below 30%. Because the performance of ultrasounds procedures was low, they cannot be recommended until further improvements are made.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support by national funds FCT/MCTES to CIMO (UIDB/00690/2020). Thanks to the Programa Apíıcola Nacional 2020-2022 (National Beekeeping Program) for funding the project "Standardization of production procedures and quality parameters of bee products" and to Project PDR2020-1.0.1- FEADER-031734: “DivInA-Diversification and Innovation on Beekeeping Production”. National funding by FCT – Foundation for Science and Technology, through the institutional scientific employment program-contract with Soraia I. Falcão. A special thanks is given to Hartmut Scheiter and Allwex Food Trading GmbH, Bremen, Germany, for providing, handling and delivering the propolis blind samples.Taylor & FrancisBiblioteca Digital do IPBVilas-Boas, MiguelLopes, MélissaNunes, L. F.Pereyra, A.Kunert, C.Beckh, G.Çelemli, Ömür GençaySorkun, KadriyeGeorgé, StéphanePaulo, LuísaGardini, SilviaSancho, M.TeresaOsés, Sandra MaríaFernandez Muiño, Miguel AngelBankova, VassyaPopova, Milena PetkovaTrusheva, Boryana S.Petrov, N.Dastan, T.Tananaki, Chrysoula H.Falcão, Soraia2023-05-18T08:25:02Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/28305engVilas-Boas, Miguel; Lopes, Mélissa; Nunes, L.F.; Pereyra, A.; Kunert, C.; Beckh, G.; Çelemli, Ömür Gençay; Sorkun, Kadriye; Georgé, Stéphane; Paulo, Luísa; Gardini, Silvia; Sancho, M. Teresa; Osés, Sandra María; Fernandez Muiño, Miguel Angel; Bankova, Vassya; Popova, Milena Petkova; Trusheva, Boryana S.; Petrov, N.; Dastan, T.; Tananaki, Chrysoula H.; Falcão, Soraia (2023). Assessing the performance of analytical methods for propolis – A collaborative trial by the international honey commission. Journal of Apicultural Research. ISSN 0021-8839. 62:3, p. 542–5550021-883910.1080/00218839.2022.2053320info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T01:20:32Zoai:bibliotecadigital.ipb.pt:10198/28305Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:16:09.437934Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Assessing the performance of analytical methods for propolis – A collaborative trial by the international honey commission |
title |
Assessing the performance of analytical methods for propolis – A collaborative trial by the international honey commission |
spellingShingle |
Assessing the performance of analytical methods for propolis – A collaborative trial by the international honey commission Vilas-Boas, Miguel Propolis Collaborative study Methods harmonization Standard methods International Honey Commission |
title_short |
Assessing the performance of analytical methods for propolis – A collaborative trial by the international honey commission |
title_full |
Assessing the performance of analytical methods for propolis – A collaborative trial by the international honey commission |
title_fullStr |
Assessing the performance of analytical methods for propolis – A collaborative trial by the international honey commission |
title_full_unstemmed |
Assessing the performance of analytical methods for propolis – A collaborative trial by the international honey commission |
title_sort |
Assessing the performance of analytical methods for propolis – A collaborative trial by the international honey commission |
author |
Vilas-Boas, Miguel |
author_facet |
Vilas-Boas, Miguel Lopes, Mélissa Nunes, L. F. Pereyra, A. Kunert, C. Beckh, G. Çelemli, Ömür Gençay Sorkun, Kadriye Georgé, Stéphane Paulo, Luísa Gardini, Silvia Sancho, M.Teresa Osés, Sandra María Fernandez Muiño, Miguel Angel Bankova, Vassya Popova, Milena Petkova Trusheva, Boryana S. Petrov, N. Dastan, T. Tananaki, Chrysoula H. Falcão, Soraia |
author_role |
author |
author2 |
Lopes, Mélissa Nunes, L. F. Pereyra, A. Kunert, C. Beckh, G. Çelemli, Ömür Gençay Sorkun, Kadriye Georgé, Stéphane Paulo, Luísa Gardini, Silvia Sancho, M.Teresa Osés, Sandra María Fernandez Muiño, Miguel Angel Bankova, Vassya Popova, Milena Petkova Trusheva, Boryana S. Petrov, N. Dastan, T. Tananaki, Chrysoula H. Falcão, Soraia |
author2_role |
author author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Vilas-Boas, Miguel Lopes, Mélissa Nunes, L. F. Pereyra, A. Kunert, C. Beckh, G. Çelemli, Ömür Gençay Sorkun, Kadriye Georgé, Stéphane Paulo, Luísa Gardini, Silvia Sancho, M.Teresa Osés, Sandra María Fernandez Muiño, Miguel Angel Bankova, Vassya Popova, Milena Petkova Trusheva, Boryana S. Petrov, N. Dastan, T. Tananaki, Chrysoula H. Falcão, Soraia |
dc.subject.por.fl_str_mv |
Propolis Collaborative study Methods harmonization Standard methods International Honey Commission |
topic |
Propolis Collaborative study Methods harmonization Standard methods International Honey Commission |
description |
Propolis is a resinous beehive product with extraordinary bioactivity and chemical richness, linked with the botanical sources of the resin. The potential of this product keeps captivating the scientific community, conducting to continuous and growing research on plant sources, composition, or applications in agriculture, cosmetics, pharmacy, odontology, etc. In all cases, the quality assessment is a requirement and relies on methods to extract the bioactive substances from the raw propolis and quantify different components. Unfortunately, besides the absence of international quality requirements, there is also a lack of standardized analytical procedures, despite the presence of several methodologies with unknown reliability, often not comparable. To overcome the current status, the International Honey Commission established an inter-laboratory study, with propolis samples from around the globe, to harmonize analytical methods and evaluate their accuracy. A common set of protocols was matched between twelve laboratories from nine countries, for quantification of ash, wax, and balsamic content in raw propolis, and spectrophotometric evaluation of total phenolics, flavone/flavonol, and flavanone/ dihydroflavonol in the extract. A total of 3428 results (97% valid data), were used to assess the methods’ accuracy following ISO-5725 guidelines. The within-laboratory precision, revealed good agreement levels for the majority of the methods, with relative variance below 5%. As expected, the between-laboratory variance increased, but, with exception of the flavanone method that revealed a clear lack of consistency, all the others maintained acceptable variability levels, below 30%. Because the performance of ultrasounds procedures was low, they cannot be recommended until further improvements are made. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-05-18T08:25:02Z 2023 2023-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/28305 |
url |
http://hdl.handle.net/10198/28305 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Vilas-Boas, Miguel; Lopes, Mélissa; Nunes, L.F.; Pereyra, A.; Kunert, C.; Beckh, G.; Çelemli, Ömür Gençay; Sorkun, Kadriye; Georgé, Stéphane; Paulo, Luísa; Gardini, Silvia; Sancho, M. Teresa; Osés, Sandra María; Fernandez Muiño, Miguel Angel; Bankova, Vassya; Popova, Milena Petkova; Trusheva, Boryana S.; Petrov, N.; Dastan, T.; Tananaki, Chrysoula H.; Falcão, Soraia (2023). Assessing the performance of analytical methods for propolis – A collaborative trial by the international honey commission. Journal of Apicultural Research. ISSN 0021-8839. 62:3, p. 542–555 0021-8839 10.1080/00218839.2022.2053320 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Taylor & Francis |
publisher.none.fl_str_mv |
Taylor & Francis |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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