Profiling the occurrence of biogenic amines in different types of tuna samples using an improved analytical approach
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.13/3866 |
Resumo: | Food deterioration caused by microbial agents often involve the formation of biogenic amines (BAs), which can have harmful effects on human health. In this study a set of BAs - tryptamine, cadaverine, putrescine, spermine, histamine, tyramine, and spermidine, were simultaneously analysed to monitor their occurrence in different types of tuna samples. An improved extraction approach involving ultrasound-assisted microextraction (USAμET) followed by derivatization with dansyl chloride (DnsCl) and analysis by ultrahigh performance liquid chroma tography (UHPLC) with fluorescence detection was validated for BAs quantification. The performance of the USAμET/UHPLC-FLD was assessed by studying the limits of detection (LOD) and quantification (LOQ), linear dynamic range (LDR), precision (intra and inter-day) and matrix effect (ME). Good linearity (r2 > 0.98), LODs (from 0.98 to 8.57 mg kg− 1 ) and LOQs (3.20–25.6 mg kg− 1 ) were achieved for all BAs analysed. Recoveries ranged from 76% to 106%, with relative standard deviations (RSD) lower than 5.0%. ME was determined from 7.52 to 50% and the intra and inter-day precisions ranged from 4.7 to 11.6% and 5.5–14.2%, respectively. BAs levels varied significantly from 4.09 ± 0.8 mg kg− 1 of putrescine in olive oil canned samples to 577.9 ± 5.9 mg kg− 1 of cadaverine in natural canned samples. Tryptamine and tyramine were not detected in any of the samples analysed, while spermine and spermidine were found in 85.7% of the assayed tuna samples. Cadaverine was the most dominant BA with concentrations ranging from 54.3 ± 2.5 mg kg− 1 , in olive oil canned tuna, to 577.9 ± 5.9 mg kg− 1 , in natural canned samples, whereas putrescine had the lowest concentration (average 6.9 ± 2.5 mg kg− 1 ). The validated methodology revealed important improvements in terms of simplification of the experi mental layout, expressed in the low sample and reagent amounts, in addition to less time-consuming and labour intensive requirements that did not compromise the analytical performance. |
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Profiling the occurrence of biogenic amines in different types of tuna samples using an improved analytical approachBiogenic amines (BAs)Tuna samplesUSAμETDnsClUHPLC-FLDFood safety.Faculdade de Ciências Exatas e da EngenhariaFaculdade de Ciências da VidaCentro de Química da MadeiraFood deterioration caused by microbial agents often involve the formation of biogenic amines (BAs), which can have harmful effects on human health. In this study a set of BAs - tryptamine, cadaverine, putrescine, spermine, histamine, tyramine, and spermidine, were simultaneously analysed to monitor their occurrence in different types of tuna samples. An improved extraction approach involving ultrasound-assisted microextraction (USAμET) followed by derivatization with dansyl chloride (DnsCl) and analysis by ultrahigh performance liquid chroma tography (UHPLC) with fluorescence detection was validated for BAs quantification. The performance of the USAμET/UHPLC-FLD was assessed by studying the limits of detection (LOD) and quantification (LOQ), linear dynamic range (LDR), precision (intra and inter-day) and matrix effect (ME). Good linearity (r2 > 0.98), LODs (from 0.98 to 8.57 mg kg− 1 ) and LOQs (3.20–25.6 mg kg− 1 ) were achieved for all BAs analysed. Recoveries ranged from 76% to 106%, with relative standard deviations (RSD) lower than 5.0%. ME was determined from 7.52 to 50% and the intra and inter-day precisions ranged from 4.7 to 11.6% and 5.5–14.2%, respectively. BAs levels varied significantly from 4.09 ± 0.8 mg kg− 1 of putrescine in olive oil canned samples to 577.9 ± 5.9 mg kg− 1 of cadaverine in natural canned samples. Tryptamine and tyramine were not detected in any of the samples analysed, while spermine and spermidine were found in 85.7% of the assayed tuna samples. Cadaverine was the most dominant BA with concentrations ranging from 54.3 ± 2.5 mg kg− 1 , in olive oil canned tuna, to 577.9 ± 5.9 mg kg− 1 , in natural canned samples, whereas putrescine had the lowest concentration (average 6.9 ± 2.5 mg kg− 1 ). The validated methodology revealed important improvements in terms of simplification of the experi mental layout, expressed in the low sample and reagent amounts, in addition to less time-consuming and labour intensive requirements that did not compromise the analytical performance.ElsevierDigitUMaPataca, Joanna K.G.Porto-Figueira, PriscillaPereira, Jorge A. M.Caldeira, HelenaCâmara, José S.2021-11-29T15:29:07Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/3866engPataca, J. K., Porto-Figueira, P., Pereira, J. A., Caldeira, H., & Câmara, J. S. (2021). Profiling the occurrence of biogenic amines in different types of tuna samples using an improved analytical approach. LWT, 139, 110804. https://doi.org/10.1016/j.lwt.2020.11080410.1016/j.lwt.2020.110804info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-17T05:57:46Zoai:digituma.uma.pt:10400.13/3866Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:07:26.308118Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Profiling the occurrence of biogenic amines in different types of tuna samples using an improved analytical approach |
title |
Profiling the occurrence of biogenic amines in different types of tuna samples using an improved analytical approach |
spellingShingle |
Profiling the occurrence of biogenic amines in different types of tuna samples using an improved analytical approach Pataca, Joanna K.G. Biogenic amines (BAs) Tuna samples USAμET DnsCl UHPLC-FLD Food safety . Faculdade de Ciências Exatas e da Engenharia Faculdade de Ciências da Vida Centro de Química da Madeira |
title_short |
Profiling the occurrence of biogenic amines in different types of tuna samples using an improved analytical approach |
title_full |
Profiling the occurrence of biogenic amines in different types of tuna samples using an improved analytical approach |
title_fullStr |
Profiling the occurrence of biogenic amines in different types of tuna samples using an improved analytical approach |
title_full_unstemmed |
Profiling the occurrence of biogenic amines in different types of tuna samples using an improved analytical approach |
title_sort |
Profiling the occurrence of biogenic amines in different types of tuna samples using an improved analytical approach |
author |
Pataca, Joanna K.G. |
author_facet |
Pataca, Joanna K.G. Porto-Figueira, Priscilla Pereira, Jorge A. M. Caldeira, Helena Câmara, José S. |
author_role |
author |
author2 |
Porto-Figueira, Priscilla Pereira, Jorge A. M. Caldeira, Helena Câmara, José S. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
DigitUMa |
dc.contributor.author.fl_str_mv |
Pataca, Joanna K.G. Porto-Figueira, Priscilla Pereira, Jorge A. M. Caldeira, Helena Câmara, José S. |
dc.subject.por.fl_str_mv |
Biogenic amines (BAs) Tuna samples USAμET DnsCl UHPLC-FLD Food safety . Faculdade de Ciências Exatas e da Engenharia Faculdade de Ciências da Vida Centro de Química da Madeira |
topic |
Biogenic amines (BAs) Tuna samples USAμET DnsCl UHPLC-FLD Food safety . Faculdade de Ciências Exatas e da Engenharia Faculdade de Ciências da Vida Centro de Química da Madeira |
description |
Food deterioration caused by microbial agents often involve the formation of biogenic amines (BAs), which can have harmful effects on human health. In this study a set of BAs - tryptamine, cadaverine, putrescine, spermine, histamine, tyramine, and spermidine, were simultaneously analysed to monitor their occurrence in different types of tuna samples. An improved extraction approach involving ultrasound-assisted microextraction (USAμET) followed by derivatization with dansyl chloride (DnsCl) and analysis by ultrahigh performance liquid chroma tography (UHPLC) with fluorescence detection was validated for BAs quantification. The performance of the USAμET/UHPLC-FLD was assessed by studying the limits of detection (LOD) and quantification (LOQ), linear dynamic range (LDR), precision (intra and inter-day) and matrix effect (ME). Good linearity (r2 > 0.98), LODs (from 0.98 to 8.57 mg kg− 1 ) and LOQs (3.20–25.6 mg kg− 1 ) were achieved for all BAs analysed. Recoveries ranged from 76% to 106%, with relative standard deviations (RSD) lower than 5.0%. ME was determined from 7.52 to 50% and the intra and inter-day precisions ranged from 4.7 to 11.6% and 5.5–14.2%, respectively. BAs levels varied significantly from 4.09 ± 0.8 mg kg− 1 of putrescine in olive oil canned samples to 577.9 ± 5.9 mg kg− 1 of cadaverine in natural canned samples. Tryptamine and tyramine were not detected in any of the samples analysed, while spermine and spermidine were found in 85.7% of the assayed tuna samples. Cadaverine was the most dominant BA with concentrations ranging from 54.3 ± 2.5 mg kg− 1 , in olive oil canned tuna, to 577.9 ± 5.9 mg kg− 1 , in natural canned samples, whereas putrescine had the lowest concentration (average 6.9 ± 2.5 mg kg− 1 ). The validated methodology revealed important improvements in terms of simplification of the experi mental layout, expressed in the low sample and reagent amounts, in addition to less time-consuming and labour intensive requirements that did not compromise the analytical performance. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-29T15:29:07Z 2021 2021-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.13/3866 |
url |
http://hdl.handle.net/10400.13/3866 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pataca, J. K., Porto-Figueira, P., Pereira, J. A., Caldeira, H., & Câmara, J. S. (2021). Profiling the occurrence of biogenic amines in different types of tuna samples using an improved analytical approach. LWT, 139, 110804. https://doi.org/10.1016/j.lwt.2020.110804 10.1016/j.lwt.2020.110804 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799129942971121664 |