Changes associated with Na,K-ATPase in brain, kidney, heart and liver of the spontaneously diabetic Goto-Kakizaki rat
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/13205 |
Resumo: | Alterations in Na,K-ATPase activity, in isoenzyme expression and/or number of units of the pump present in the plasma membrane have been associated with diabetes. These changes were described in different organs and tissues such as brain, heart, kidney, among others, and may result from altered insulin levels. However, the vast majority of studies were conducted in animal models of chemically induced diabetes, which are not consensual models for type 2 diabetes (T2D). The major goal of this work was to investigate putative modifications in Na,K-ATPase enzymatic activity or expression in brain, kidney, heart and liver in T2D. The Goto-Kakizaky rat (GK) strain was used as a model of spontaneously developed T2D, and Wistar rats as controls. Na,K-ATPase activity was assessed by the hydrolysis of ATP (Pi formed in the presence/absence of ouabain was measured using a colorimetric assay) and the isoenzymatic expression by Westernblot. A decrease in Na,K-ATPase activity in renal and cardiac tissues from GK comparatively to controls (55.7% and 77.5%, respectively) was observed. The pump activity was similar in liver and brain tissues. In renal tissue, expression of α1-Na,K-ATPase was similar between GK and controls but α2- was 2.3x higher and α3- was detected only in GK. Contrastingly a decreased expression of α1- (49.5%) and α2-isoforms (67.6%) was found in cardiac tissue. Despite similar Na,K-ATPase activity in liver and brain tissues, α1-isoform expression was decreased (33.9%) in the liver from GK while in the brain an increase of α1-isoform (~2x) together with a decrease of α2-isoform (14.0%) expressions were observed. It is unclear, except maybe for cardiac tissue where lower expression is potentially underlying the diminished pump activity in GK, whether the changes in isoenzyme expression is a key factor for differential Na,K-ATPase activity. Being responsive to complex regulation, other regulatory mechanisms may contribute to the impaired activity observed in kidney and heart. These results have uncover changes in Na,K-ATPase activity and/or enzymatic expression in GK brain, kidney, heart and liver that may contribute to the undesirable conditions associated with T2D. This work highlight the relevance of further investigation about Na,K-ATPase regulation and role in physiopathology of T2D. |
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Changes associated with Na,K-ATPase in brain, kidney, heart and liver of the spontaneously diabetic Goto-Kakizaki ratNa,K-ATPasediabetesAlterations in Na,K-ATPase activity, in isoenzyme expression and/or number of units of the pump present in the plasma membrane have been associated with diabetes. These changes were described in different organs and tissues such as brain, heart, kidney, among others, and may result from altered insulin levels. However, the vast majority of studies were conducted in animal models of chemically induced diabetes, which are not consensual models for type 2 diabetes (T2D). The major goal of this work was to investigate putative modifications in Na,K-ATPase enzymatic activity or expression in brain, kidney, heart and liver in T2D. The Goto-Kakizaky rat (GK) strain was used as a model of spontaneously developed T2D, and Wistar rats as controls. Na,K-ATPase activity was assessed by the hydrolysis of ATP (Pi formed in the presence/absence of ouabain was measured using a colorimetric assay) and the isoenzymatic expression by Westernblot. A decrease in Na,K-ATPase activity in renal and cardiac tissues from GK comparatively to controls (55.7% and 77.5%, respectively) was observed. The pump activity was similar in liver and brain tissues. In renal tissue, expression of α1-Na,K-ATPase was similar between GK and controls but α2- was 2.3x higher and α3- was detected only in GK. Contrastingly a decreased expression of α1- (49.5%) and α2-isoforms (67.6%) was found in cardiac tissue. Despite similar Na,K-ATPase activity in liver and brain tissues, α1-isoform expression was decreased (33.9%) in the liver from GK while in the brain an increase of α1-isoform (~2x) together with a decrease of α2-isoform (14.0%) expressions were observed. It is unclear, except maybe for cardiac tissue where lower expression is potentially underlying the diminished pump activity in GK, whether the changes in isoenzyme expression is a key factor for differential Na,K-ATPase activity. Being responsive to complex regulation, other regulatory mechanisms may contribute to the impaired activity observed in kidney and heart. These results have uncover changes in Na,K-ATPase activity and/or enzymatic expression in GK brain, kidney, heart and liver that may contribute to the undesirable conditions associated with T2D. This work highlight the relevance of further investigation about Na,K-ATPase regulation and role in physiopathology of T2D.Sociedade Portuguesa de Bioquímica2015-03-09T11:34:59Z2015-03-092014-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/13205http://hdl.handle.net/10174/13205engRoque, C, Costa A.R., Capela-Silva, F., Antunes C.M. (2014) Changes associated with Na,K-ATPase in brain, kidney, heart and liver of the spontaneously diabetic Goto-Kakizaki rat. XVIII Congress of the Portuguese Biochemical Society, 17-20 Dec, Coimbra, Portugal, K.17, pp 121 - Poster.http://spb2014.com/media/AbstractBook_fv.pdfnaonaosimkatuxinha_st_star_69@hotmail.comacrc@uevora.ptfcs@uevora.ptcmma@uevora.pt365Roque, CátiaCosta, Ana R.Capela-Silva, FernandoAntunes, Célia Minfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:58:26Zoai:dspace.uevora.pt:10174/13205Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:06:37.759917Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Changes associated with Na,K-ATPase in brain, kidney, heart and liver of the spontaneously diabetic Goto-Kakizaki rat |
title |
Changes associated with Na,K-ATPase in brain, kidney, heart and liver of the spontaneously diabetic Goto-Kakizaki rat |
spellingShingle |
Changes associated with Na,K-ATPase in brain, kidney, heart and liver of the spontaneously diabetic Goto-Kakizaki rat Roque, Cátia Na,K-ATPase diabetes |
title_short |
Changes associated with Na,K-ATPase in brain, kidney, heart and liver of the spontaneously diabetic Goto-Kakizaki rat |
title_full |
Changes associated with Na,K-ATPase in brain, kidney, heart and liver of the spontaneously diabetic Goto-Kakizaki rat |
title_fullStr |
Changes associated with Na,K-ATPase in brain, kidney, heart and liver of the spontaneously diabetic Goto-Kakizaki rat |
title_full_unstemmed |
Changes associated with Na,K-ATPase in brain, kidney, heart and liver of the spontaneously diabetic Goto-Kakizaki rat |
title_sort |
Changes associated with Na,K-ATPase in brain, kidney, heart and liver of the spontaneously diabetic Goto-Kakizaki rat |
author |
Roque, Cátia |
author_facet |
Roque, Cátia Costa, Ana R. Capela-Silva, Fernando Antunes, Célia M |
author_role |
author |
author2 |
Costa, Ana R. Capela-Silva, Fernando Antunes, Célia M |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Roque, Cátia Costa, Ana R. Capela-Silva, Fernando Antunes, Célia M |
dc.subject.por.fl_str_mv |
Na,K-ATPase diabetes |
topic |
Na,K-ATPase diabetes |
description |
Alterations in Na,K-ATPase activity, in isoenzyme expression and/or number of units of the pump present in the plasma membrane have been associated with diabetes. These changes were described in different organs and tissues such as brain, heart, kidney, among others, and may result from altered insulin levels. However, the vast majority of studies were conducted in animal models of chemically induced diabetes, which are not consensual models for type 2 diabetes (T2D). The major goal of this work was to investigate putative modifications in Na,K-ATPase enzymatic activity or expression in brain, kidney, heart and liver in T2D. The Goto-Kakizaky rat (GK) strain was used as a model of spontaneously developed T2D, and Wistar rats as controls. Na,K-ATPase activity was assessed by the hydrolysis of ATP (Pi formed in the presence/absence of ouabain was measured using a colorimetric assay) and the isoenzymatic expression by Westernblot. A decrease in Na,K-ATPase activity in renal and cardiac tissues from GK comparatively to controls (55.7% and 77.5%, respectively) was observed. The pump activity was similar in liver and brain tissues. In renal tissue, expression of α1-Na,K-ATPase was similar between GK and controls but α2- was 2.3x higher and α3- was detected only in GK. Contrastingly a decreased expression of α1- (49.5%) and α2-isoforms (67.6%) was found in cardiac tissue. Despite similar Na,K-ATPase activity in liver and brain tissues, α1-isoform expression was decreased (33.9%) in the liver from GK while in the brain an increase of α1-isoform (~2x) together with a decrease of α2-isoform (14.0%) expressions were observed. It is unclear, except maybe for cardiac tissue where lower expression is potentially underlying the diminished pump activity in GK, whether the changes in isoenzyme expression is a key factor for differential Na,K-ATPase activity. Being responsive to complex regulation, other regulatory mechanisms may contribute to the impaired activity observed in kidney and heart. These results have uncover changes in Na,K-ATPase activity and/or enzymatic expression in GK brain, kidney, heart and liver that may contribute to the undesirable conditions associated with T2D. This work highlight the relevance of further investigation about Na,K-ATPase regulation and role in physiopathology of T2D. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-01T00:00:00Z 2015-03-09T11:34:59Z 2015-03-09 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/13205 http://hdl.handle.net/10174/13205 |
url |
http://hdl.handle.net/10174/13205 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Roque, C, Costa A.R., Capela-Silva, F., Antunes C.M. (2014) Changes associated with Na,K-ATPase in brain, kidney, heart and liver of the spontaneously diabetic Goto-Kakizaki rat. XVIII Congress of the Portuguese Biochemical Society, 17-20 Dec, Coimbra, Portugal, K.17, pp 121 - Poster. http://spb2014.com/media/AbstractBook_fv.pdf nao nao sim katuxinha_st_star_69@hotmail.com acrc@uevora.pt fcs@uevora.pt cmma@uevora.pt 365 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Sociedade Portuguesa de Bioquímica |
publisher.none.fl_str_mv |
Sociedade Portuguesa de Bioquímica |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136551112802304 |