Antiproliferative Activity of Buddleja saligna (Willd.) against Melanoma and In Vivo Modulation of Angiogenesis

Detalhes bibliográficos
Autor(a) principal: Twilley, Danielle
Data de Publicação: 2022
Outros Autores: Thipe, Velaphi C., Kishore, Navneet, Bloebaum, Pierce, Roma-Rodrigues, Catarina, Baptista, Pedro V., Fernandes, Alexandra R., Selepe, Mamoalosi A., Langhansova, Lenka, Katti, Kattesh, Lall, Namrita
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/150557
Resumo: This research was funded by the University of Pretoria, the National Research Foundation-DAAD (SFD13080220333), the Department of Science and Innovation (DST/CON 0059/2019), the Innovation Hub, L’Oréal-UNESCO. Publisher Copyright: © 2022 by the authors.
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spelling Antiproliferative Activity of Buddleja saligna (Willd.) against Melanoma and In Vivo Modulation of Angiogenesisangiogenesisantiproliferative activityBuddleja salignaex ovo YSMmelanomaMolecular MedicinePharmaceutical ScienceDrug DiscoverySDG 3 - Good Health and Well-beingThis research was funded by the University of Pretoria, the National Research Foundation-DAAD (SFD13080220333), the Department of Science and Innovation (DST/CON 0059/2019), the Innovation Hub, L’Oréal-UNESCO. Publisher Copyright: © 2022 by the authors.Melanoma cells secrete pro-angiogenic factors, which stimulates growth, proliferation and metastasis, and therefore are key therapeutic targets. Buddleja saligna (BS), and an isolated triterpenoid mixture (DT-BS-01) showed a fifty percent inhibitory concentration (IC50) of 33.80 ± 1.02 and 5.45 ± 0.19 µg/mL, respectively, against melanoma cells (UCT-MEL-1) with selectivity index (SI) values of 1.64 and 5.06 compared to keratinocytes (HaCat). Cyclooxygenase-2 (COX-2) inhibition was observed with IC50 values of 35.06 ± 2.96 (BS) and 26.40 ± 4.19 µg/mL (DT-BS-01). BS (30 µg/mL) significantly inhibited interleukin (IL)-6 (83.26 ± 17.60%) and IL-8 (100 ± 0.2%) production, whereas DT-BS-01 (5 µg/mL) showed 51.07 ± 2.83 (IL-6) and 0 ± 6.7% (IL-8) inhibition. Significant vascular endothelial growth factor (VEGF) inhibition, by 15.84 ± 4.54 and 12.21 ± 3.48%, respectively, was observed. In the ex ovo chick embryo yolk sac membrane assay (YSM), BS (15 µg/egg) significantly reduced new blood vessel formation, with 53.34 ± 11.64% newly formed vessels. Silver and palladium BS nanoparticles displayed noteworthy SI values. This is the first report on the significant anti-angiogenic activity of BS and DT-BS-01 and should be considered for preclinical trials as there are currently no US Food and Drug Administration (FDA) approved drugs to inhibit angiogenesis in melanoma.UCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaRUNTwilley, DanielleThipe, Velaphi C.Kishore, NavneetBloebaum, PierceRoma-Rodrigues, CatarinaBaptista, Pedro V.Fernandes, Alexandra R.Selepe, Mamoalosi A.Langhansova, LenkaKatti, KatteshLall, Namrita2023-03-14T22:43:03Z2022-11-302022-11-30T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article24application/pdfhttp://hdl.handle.net/10362/150557eng1424-8247PURE: 55634892https://doi.org/10.3390/ph15121497info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:32:34Zoai:run.unl.pt:10362/150557Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:54:10.799850Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Antiproliferative Activity of Buddleja saligna (Willd.) against Melanoma and In Vivo Modulation of Angiogenesis
title Antiproliferative Activity of Buddleja saligna (Willd.) against Melanoma and In Vivo Modulation of Angiogenesis
spellingShingle Antiproliferative Activity of Buddleja saligna (Willd.) against Melanoma and In Vivo Modulation of Angiogenesis
Twilley, Danielle
angiogenesis
antiproliferative activity
Buddleja saligna
ex ovo YSM
melanoma
Molecular Medicine
Pharmaceutical Science
Drug Discovery
SDG 3 - Good Health and Well-being
title_short Antiproliferative Activity of Buddleja saligna (Willd.) against Melanoma and In Vivo Modulation of Angiogenesis
title_full Antiproliferative Activity of Buddleja saligna (Willd.) against Melanoma and In Vivo Modulation of Angiogenesis
title_fullStr Antiproliferative Activity of Buddleja saligna (Willd.) against Melanoma and In Vivo Modulation of Angiogenesis
title_full_unstemmed Antiproliferative Activity of Buddleja saligna (Willd.) against Melanoma and In Vivo Modulation of Angiogenesis
title_sort Antiproliferative Activity of Buddleja saligna (Willd.) against Melanoma and In Vivo Modulation of Angiogenesis
author Twilley, Danielle
author_facet Twilley, Danielle
Thipe, Velaphi C.
Kishore, Navneet
Bloebaum, Pierce
Roma-Rodrigues, Catarina
Baptista, Pedro V.
Fernandes, Alexandra R.
Selepe, Mamoalosi A.
Langhansova, Lenka
Katti, Kattesh
Lall, Namrita
author_role author
author2 Thipe, Velaphi C.
Kishore, Navneet
Bloebaum, Pierce
Roma-Rodrigues, Catarina
Baptista, Pedro V.
Fernandes, Alexandra R.
Selepe, Mamoalosi A.
Langhansova, Lenka
Katti, Kattesh
Lall, Namrita
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DCV - Departamento de Ciências da Vida
RUN
dc.contributor.author.fl_str_mv Twilley, Danielle
Thipe, Velaphi C.
Kishore, Navneet
Bloebaum, Pierce
Roma-Rodrigues, Catarina
Baptista, Pedro V.
Fernandes, Alexandra R.
Selepe, Mamoalosi A.
Langhansova, Lenka
Katti, Kattesh
Lall, Namrita
dc.subject.por.fl_str_mv angiogenesis
antiproliferative activity
Buddleja saligna
ex ovo YSM
melanoma
Molecular Medicine
Pharmaceutical Science
Drug Discovery
SDG 3 - Good Health and Well-being
topic angiogenesis
antiproliferative activity
Buddleja saligna
ex ovo YSM
melanoma
Molecular Medicine
Pharmaceutical Science
Drug Discovery
SDG 3 - Good Health and Well-being
description This research was funded by the University of Pretoria, the National Research Foundation-DAAD (SFD13080220333), the Department of Science and Innovation (DST/CON 0059/2019), the Innovation Hub, L’Oréal-UNESCO. Publisher Copyright: © 2022 by the authors.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-30
2022-11-30T00:00:00Z
2023-03-14T22:43:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/150557
url http://hdl.handle.net/10362/150557
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1424-8247
PURE: 55634892
https://doi.org/10.3390/ph15121497
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 24
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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