Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 mice
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/26067 |
Resumo: | Obesity is an epidemic disease worldwide, associated with oxidative stress and the development of several other diseases. Bauhinia rufa (Bong.) Steud. is a native Brazilian Cerrado medicinal plant popularly used for the treatment of obesity. In this context, we investigated the chemical composition of the methanolic extract of B. rufa leaves (MEBr) and evaluated the antioxidant activity and its impact on the prevention and treatment of obesity in mice fed a high-fat diet (HFD 60%). Additionally, the acute oral toxicity of MEBr was evaluated. In MEBr, 17 glycosylated compounds were identified, including myricetin, quercetin, kaempferol, coumaroyl, cyanoglucoside, and megastigmane. In vitro, MEBr showed antioxidant activity in different methods: DPPH•, ABTS•+, FRAP, iron-reducing power, inhibition of β-carotene bleaching, and inhibition of DNA fragmentation. In human erythrocytes, MEBr increased the activities of antioxidant enzymes, superoxide dismutase, and catalase. Under oxidative stress, MEBr reduced oxidative hemolysis, and the malondialdehyde (MDA) levels generated in erythrocytes. Mice treated acutely with MEBr (2000 mg/kg) showed no signs of toxicity. During 90 days, the mice received water or MEBr simultaneously with HFD for induction of obesity. At this stage, MEBr was able to reduce the gain of subcutaneous white adipose tissue (WAT) and prevent the increase of MDA in the heart and brain. After 180 days of HFD for obesity induction, mice that received MEBr simultaneously with HFD (HFD-MEBr) in the last 60 days of treatment (120-180 days) showed a reduction of retroperitoneal and mesenteric WAT deposits and MDA levels in the heart, liver, kidney, and brain, compared to the HFD-Control group. These effects of MEBr were similar to mice treated with sibutramine (HFD-Sibutramine, 2 mg/kg). Combined, the results show that compounds from the leaves of B. rufa affect controlling oxidative stress and actions in the prevention and treatment of obesity. Thus, associated oxidative stress reduction and body composition modulation, in obese people, can contribute to the prevention of obesity-related comorbidities and improve quality of life. |
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Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 miceAntioxidantsBloodFlavonoidsQuality ControlObesity is an epidemic disease worldwide, associated with oxidative stress and the development of several other diseases. Bauhinia rufa (Bong.) Steud. is a native Brazilian Cerrado medicinal plant popularly used for the treatment of obesity. In this context, we investigated the chemical composition of the methanolic extract of B. rufa leaves (MEBr) and evaluated the antioxidant activity and its impact on the prevention and treatment of obesity in mice fed a high-fat diet (HFD 60%). Additionally, the acute oral toxicity of MEBr was evaluated. In MEBr, 17 glycosylated compounds were identified, including myricetin, quercetin, kaempferol, coumaroyl, cyanoglucoside, and megastigmane. In vitro, MEBr showed antioxidant activity in different methods: DPPH•, ABTS•+, FRAP, iron-reducing power, inhibition of β-carotene bleaching, and inhibition of DNA fragmentation. In human erythrocytes, MEBr increased the activities of antioxidant enzymes, superoxide dismutase, and catalase. Under oxidative stress, MEBr reduced oxidative hemolysis, and the malondialdehyde (MDA) levels generated in erythrocytes. Mice treated acutely with MEBr (2000 mg/kg) showed no signs of toxicity. During 90 days, the mice received water or MEBr simultaneously with HFD for induction of obesity. At this stage, MEBr was able to reduce the gain of subcutaneous white adipose tissue (WAT) and prevent the increase of MDA in the heart and brain. After 180 days of HFD for obesity induction, mice that received MEBr simultaneously with HFD (HFD-MEBr) in the last 60 days of treatment (120-180 days) showed a reduction of retroperitoneal and mesenteric WAT deposits and MDA levels in the heart, liver, kidney, and brain, compared to the HFD-Control group. These effects of MEBr were similar to mice treated with sibutramine (HFD-Sibutramine, 2 mg/kg). Combined, the results show that compounds from the leaves of B. rufa affect controlling oxidative stress and actions in the prevention and treatment of obesity. Thus, associated oxidative stress reduction and body composition modulation, in obese people, can contribute to the prevention of obesity-related comorbidities and improve quality of life.HindawiBiblioteca Digital do IPBRocha, Paola dos SantosOrué, Sarah LamLeite, Daniel FerreiraEspindola, Priscilla Pereira de ToledoCassemiro, Nadla SoaresSilva, Denise BrentanCarollo, Carlos AlexandreNunes-Souza, ValériaRabelo, Luiza AntasCampos, Jaqueline FerreiraEstevinho, Leticia M.Santos, Edson Lucas dos2022-11-04T10:38:05Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/26067engRocha, Paola dos Santos da; Orué, Sarah Lam; Leite, Daniel Ferreira; Espindola, Priscilla Pereira de Toledo; Cassemiro, Nadla Soares; Silva, Denise Brentan da; Carollo, Carlos Alexandre; Nunes-Souza, Valéria; Rabelo, Luiza Antas; Campos, Jaqueline Ferreira; Estevinho, Leticia M.; Santos, Edson Lucas dos. (2022). Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 mice. Oxidative Medicine and Cellular Longevity. ISSN 19420900. 2022, p. 1-1319420900info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:58:16Zoai:bibliotecadigital.ipb.pt:10198/26067Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:16:36.879413Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 mice |
title |
Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 mice |
spellingShingle |
Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 mice Rocha, Paola dos Santos Antioxidants Blood Flavonoids Quality Control |
title_short |
Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 mice |
title_full |
Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 mice |
title_fullStr |
Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 mice |
title_full_unstemmed |
Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 mice |
title_sort |
Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 mice |
author |
Rocha, Paola dos Santos |
author_facet |
Rocha, Paola dos Santos Orué, Sarah Lam Leite, Daniel Ferreira Espindola, Priscilla Pereira de Toledo Cassemiro, Nadla Soares Silva, Denise Brentan Carollo, Carlos Alexandre Nunes-Souza, Valéria Rabelo, Luiza Antas Campos, Jaqueline Ferreira Estevinho, Leticia M. Santos, Edson Lucas dos |
author_role |
author |
author2 |
Orué, Sarah Lam Leite, Daniel Ferreira Espindola, Priscilla Pereira de Toledo Cassemiro, Nadla Soares Silva, Denise Brentan Carollo, Carlos Alexandre Nunes-Souza, Valéria Rabelo, Luiza Antas Campos, Jaqueline Ferreira Estevinho, Leticia M. Santos, Edson Lucas dos |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Rocha, Paola dos Santos Orué, Sarah Lam Leite, Daniel Ferreira Espindola, Priscilla Pereira de Toledo Cassemiro, Nadla Soares Silva, Denise Brentan Carollo, Carlos Alexandre Nunes-Souza, Valéria Rabelo, Luiza Antas Campos, Jaqueline Ferreira Estevinho, Leticia M. Santos, Edson Lucas dos |
dc.subject.por.fl_str_mv |
Antioxidants Blood Flavonoids Quality Control |
topic |
Antioxidants Blood Flavonoids Quality Control |
description |
Obesity is an epidemic disease worldwide, associated with oxidative stress and the development of several other diseases. Bauhinia rufa (Bong.) Steud. is a native Brazilian Cerrado medicinal plant popularly used for the treatment of obesity. In this context, we investigated the chemical composition of the methanolic extract of B. rufa leaves (MEBr) and evaluated the antioxidant activity and its impact on the prevention and treatment of obesity in mice fed a high-fat diet (HFD 60%). Additionally, the acute oral toxicity of MEBr was evaluated. In MEBr, 17 glycosylated compounds were identified, including myricetin, quercetin, kaempferol, coumaroyl, cyanoglucoside, and megastigmane. In vitro, MEBr showed antioxidant activity in different methods: DPPH•, ABTS•+, FRAP, iron-reducing power, inhibition of β-carotene bleaching, and inhibition of DNA fragmentation. In human erythrocytes, MEBr increased the activities of antioxidant enzymes, superoxide dismutase, and catalase. Under oxidative stress, MEBr reduced oxidative hemolysis, and the malondialdehyde (MDA) levels generated in erythrocytes. Mice treated acutely with MEBr (2000 mg/kg) showed no signs of toxicity. During 90 days, the mice received water or MEBr simultaneously with HFD for induction of obesity. At this stage, MEBr was able to reduce the gain of subcutaneous white adipose tissue (WAT) and prevent the increase of MDA in the heart and brain. After 180 days of HFD for obesity induction, mice that received MEBr simultaneously with HFD (HFD-MEBr) in the last 60 days of treatment (120-180 days) showed a reduction of retroperitoneal and mesenteric WAT deposits and MDA levels in the heart, liver, kidney, and brain, compared to the HFD-Control group. These effects of MEBr were similar to mice treated with sibutramine (HFD-Sibutramine, 2 mg/kg). Combined, the results show that compounds from the leaves of B. rufa affect controlling oxidative stress and actions in the prevention and treatment of obesity. Thus, associated oxidative stress reduction and body composition modulation, in obese people, can contribute to the prevention of obesity-related comorbidities and improve quality of life. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-04T10:38:05Z 2022 2022-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/26067 |
url |
http://hdl.handle.net/10198/26067 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Rocha, Paola dos Santos da; Orué, Sarah Lam; Leite, Daniel Ferreira; Espindola, Priscilla Pereira de Toledo; Cassemiro, Nadla Soares; Silva, Denise Brentan da; Carollo, Carlos Alexandre; Nunes-Souza, Valéria; Rabelo, Luiza Antas; Campos, Jaqueline Ferreira; Estevinho, Leticia M.; Santos, Edson Lucas dos. (2022). Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 mice. Oxidative Medicine and Cellular Longevity. ISSN 19420900. 2022, p. 1-13 19420900 |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Hindawi |
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Hindawi |
dc.source.none.fl_str_mv |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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