Brachyury identifies a class of enteroendocrine cells in normal human intestinal crypts and colorectal cancer
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/45060 |
Resumo: | Normal homeostasis of adult intestinal epithelium and repair following tissue damage is maintained by a balance of stem and differentiated cells, many of which are still only poorly characterised. Enteroendocrine cells of the gut are a small population of differentiated, secretory cells that are critical for integrating nutrient sensing with metabolic responses, dispersed amongst other epithelial cells. Recent evidence suggests that sub-sets of secretory enteroendocrine cells can act as reserve stem cells. Given the link between cells with stem-like properties and cancer, it is important that we identify factors that might provide a bridge between the two. Here, we identify a sub-set of chromogranin A-positive enteroendocrine cells that are positive for the developmental and cancer-associated transcription factor Brachyury in normal human small intestinal and colonic crypts. Whilst chromogranin A-positive enteroendocrine cells are also Brachyury-positive in colorectal tumours, expression of Brachyury becomes more diffuse in these samples, suggesting a more widespread function in cancer. The finding of the developmental transcription factor Brachyury in normal adult human intestinal crypts may extend the functional complexity of enteroendocrine cells and serves as a platform for assessment of the molecular processes of intestinal homeostasis that underpins our understanding of human health, cancer and aging. |
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Brachyury identifies a class of enteroendocrine cells in normal human intestinal crypts and colorectal cancerBrachyuryEnteroendocrine cellsSmall intestine/colonCryptsColorectal cancerScience & TechnologyNormal homeostasis of adult intestinal epithelium and repair following tissue damage is maintained by a balance of stem and differentiated cells, many of which are still only poorly characterised. Enteroendocrine cells of the gut are a small population of differentiated, secretory cells that are critical for integrating nutrient sensing with metabolic responses, dispersed amongst other epithelial cells. Recent evidence suggests that sub-sets of secretory enteroendocrine cells can act as reserve stem cells. Given the link between cells with stem-like properties and cancer, it is important that we identify factors that might provide a bridge between the two. Here, we identify a sub-set of chromogranin A-positive enteroendocrine cells that are positive for the developmental and cancer-associated transcription factor Brachyury in normal human small intestinal and colonic crypts. Whilst chromogranin A-positive enteroendocrine cells are also Brachyury-positive in colorectal tumours, expression of Brachyury becomes more diffuse in these samples, suggesting a more widespread function in cancer. The finding of the developmental transcription factor Brachyury in normal adult human intestinal crypts may extend the functional complexity of enteroendocrine cells and serves as a platform for assessment of the molecular processes of intestinal homeostasis that underpins our understanding of human health, cancer and aging.The authors would like to thank the following organisations for supporting this work: J. Jezkova, R.J. McFarlane & J.A. Wakeman are supported by Cancer Research Wales; J.S. Williams is supported by Coleg Cymraeg Cenedlaethol; J. Sammut and R.J. McFarlane are supported by NWCR (grant no. CR950); S. Gollins is supported by National Institute for Social Care and Health Research Academic Health Science Collaboration (NISCHR AHSC) Clinical Research Fellow. F.Pinto is supported by Fundação para a Ciência e Tecnologia (FCT; grant no. SFRH/BD/81369/2011). R.M. Reis is recipient of a Brazilian National Counsel of Technological and Scientific Development (CNPq) scholarship. Geraint Williams is supported by the Wales Gene Park. are supported by NWCR (grant no. CR950); S. Gollins is supported by National Institute for Social Care and Health Research Academic Health Science Collaboration (NISCHR AHSC) Clinical Research Fellow. F.Pinto is supported by Fundação para a Ciência e Tecnologia (FCT; grant no. SFRH/BD/81369/2011). R.M. Reis is recipient of a Brazilian National Counsel of Technological and Scientific Development (CNPq) scholarship. Geraint Williams is supported by the Wales Gene Park.info:eu-repo/semantics/publishedVersionImpact Journals LLCUniversidade do MinhoJezkova, JanaWilliams, Jason S.Pinto, Filipe Manuel TeixeiraSammut Stephen J.Williams, Geraint T.Gollins, SimonMcFarlane, Ramsay J.Reis, R. M.Wakeman, Jane A.2016-02-142016-02-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/45060engJezkova, J., Williams, J. S., Pinto, F., Sammut, S. J., Williams, G. T., Gollins, S., . . . Wakeman, J. A. (2016). Brachyury identifies a class of enteroendocrine cells in normal human intestinal crypts and colorectal cancer. Oncotarget, 7(10), 11478-11486. doi: 10.18632/oncotarget.72021949-255310.18632/oncotarget.720226862851http://www.impactjournals.com/oncotargetinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:07:56Zoai:repositorium.sdum.uminho.pt:1822/45060Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:59:01.990241Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Brachyury identifies a class of enteroendocrine cells in normal human intestinal crypts and colorectal cancer |
title |
Brachyury identifies a class of enteroendocrine cells in normal human intestinal crypts and colorectal cancer |
spellingShingle |
Brachyury identifies a class of enteroendocrine cells in normal human intestinal crypts and colorectal cancer Jezkova, Jana Brachyury Enteroendocrine cells Small intestine/colon Crypts Colorectal cancer Science & Technology |
title_short |
Brachyury identifies a class of enteroendocrine cells in normal human intestinal crypts and colorectal cancer |
title_full |
Brachyury identifies a class of enteroendocrine cells in normal human intestinal crypts and colorectal cancer |
title_fullStr |
Brachyury identifies a class of enteroendocrine cells in normal human intestinal crypts and colorectal cancer |
title_full_unstemmed |
Brachyury identifies a class of enteroendocrine cells in normal human intestinal crypts and colorectal cancer |
title_sort |
Brachyury identifies a class of enteroendocrine cells in normal human intestinal crypts and colorectal cancer |
author |
Jezkova, Jana |
author_facet |
Jezkova, Jana Williams, Jason S. Pinto, Filipe Manuel Teixeira Sammut Stephen J. Williams, Geraint T. Gollins, Simon McFarlane, Ramsay J. Reis, R. M. Wakeman, Jane A. |
author_role |
author |
author2 |
Williams, Jason S. Pinto, Filipe Manuel Teixeira Sammut Stephen J. Williams, Geraint T. Gollins, Simon McFarlane, Ramsay J. Reis, R. M. Wakeman, Jane A. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Jezkova, Jana Williams, Jason S. Pinto, Filipe Manuel Teixeira Sammut Stephen J. Williams, Geraint T. Gollins, Simon McFarlane, Ramsay J. Reis, R. M. Wakeman, Jane A. |
dc.subject.por.fl_str_mv |
Brachyury Enteroendocrine cells Small intestine/colon Crypts Colorectal cancer Science & Technology |
topic |
Brachyury Enteroendocrine cells Small intestine/colon Crypts Colorectal cancer Science & Technology |
description |
Normal homeostasis of adult intestinal epithelium and repair following tissue damage is maintained by a balance of stem and differentiated cells, many of which are still only poorly characterised. Enteroendocrine cells of the gut are a small population of differentiated, secretory cells that are critical for integrating nutrient sensing with metabolic responses, dispersed amongst other epithelial cells. Recent evidence suggests that sub-sets of secretory enteroendocrine cells can act as reserve stem cells. Given the link between cells with stem-like properties and cancer, it is important that we identify factors that might provide a bridge between the two. Here, we identify a sub-set of chromogranin A-positive enteroendocrine cells that are positive for the developmental and cancer-associated transcription factor Brachyury in normal human small intestinal and colonic crypts. Whilst chromogranin A-positive enteroendocrine cells are also Brachyury-positive in colorectal tumours, expression of Brachyury becomes more diffuse in these samples, suggesting a more widespread function in cancer. The finding of the developmental transcription factor Brachyury in normal adult human intestinal crypts may extend the functional complexity of enteroendocrine cells and serves as a platform for assessment of the molecular processes of intestinal homeostasis that underpins our understanding of human health, cancer and aging. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-02-14 2016-02-14T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/45060 |
url |
http://hdl.handle.net/1822/45060 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Jezkova, J., Williams, J. S., Pinto, F., Sammut, S. J., Williams, G. T., Gollins, S., . . . Wakeman, J. A. (2016). Brachyury identifies a class of enteroendocrine cells in normal human intestinal crypts and colorectal cancer. Oncotarget, 7(10), 11478-11486. doi: 10.18632/oncotarget.7202 1949-2553 10.18632/oncotarget.7202 26862851 http://www.impactjournals.com/oncotarget |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Impact Journals LLC |
publisher.none.fl_str_mv |
Impact Journals LLC |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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