Effect of functional feed additive on symptoms reduction of enteromyxosis in gilthead seabream “sparus aurata” (field experiment)

Detalhes bibliográficos
Autor(a) principal: Pierantozzi, Mirko
Data de Publicação: 2022
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/18782
Resumo: Aquaculture is a fast-growing sector, very important for the food, nutrition, income, and livelihood of hundreds of millions of people. The demand for seafood is increasing and aquaculture plays an important role in satisfying that. The increasing number of fish farmed go at the same pace as the health and welfare problems especially because of the high density of the fish. One of the biggest sanitary problems for the gilthead seabream industry is Enteromyxum leei, a myxosporean parasite since the infection has a chronic course and is responsible for poor food conversion rate and difficulty to reach commercial size. Unfortunately, no treatments are authorized for this parasitosis, but a recent experimental study shows a good result using SANACORE® functional feed as a treatment for this parasitosis. This study aims to verify the efficacy and the feasibility of the functional feed as a possible treatment for E. leei. The trial was done in an important commercial facility of gilthead seabream in Tuscany (Italy) where enteromyxosis has been already diagnosed in the past. For this study, two different concentrations of SANACORE® are tested, respectively 0.5% and 0.7%. Prevalence and intensity of the parasite in the fish are evaluated using microscopy observation and molecular diagnosis (PCR) to combine qualitative and quantitative parasitological analyses comparing the groups under study. The weight and length of all the fish sampled were recorded and a biomass index was measured and compared along with the different fish groups. Unfortunately, the occurrence of a severe secondary bacterial infection influenced the evaluation of the efficacy of SANACORE's inclusion in fish feed. Concerning the comparison of the different diagnostic methods, the results showed that the qPCR is a more suitable method for the diagnosis of Enteromyxosis. Because of the possibility of a non-lethal technic to collect the samples (by rectal swab), it can be used as a valid screening test in a farm for newly introduced fish.
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spelling Effect of functional feed additive on symptoms reduction of enteromyxosis in gilthead seabream “sparus aurata” (field experiment)Mediterranean aquacultureGilthead seabreamEnteromyxum leeiFunctional feedSustainable aquacultureDomínio/Área Científica::Ciências Naturais::Outras Ciências NaturaisAquaculture is a fast-growing sector, very important for the food, nutrition, income, and livelihood of hundreds of millions of people. The demand for seafood is increasing and aquaculture plays an important role in satisfying that. The increasing number of fish farmed go at the same pace as the health and welfare problems especially because of the high density of the fish. One of the biggest sanitary problems for the gilthead seabream industry is Enteromyxum leei, a myxosporean parasite since the infection has a chronic course and is responsible for poor food conversion rate and difficulty to reach commercial size. Unfortunately, no treatments are authorized for this parasitosis, but a recent experimental study shows a good result using SANACORE® functional feed as a treatment for this parasitosis. This study aims to verify the efficacy and the feasibility of the functional feed as a possible treatment for E. leei. The trial was done in an important commercial facility of gilthead seabream in Tuscany (Italy) where enteromyxosis has been already diagnosed in the past. For this study, two different concentrations of SANACORE® are tested, respectively 0.5% and 0.7%. Prevalence and intensity of the parasite in the fish are evaluated using microscopy observation and molecular diagnosis (PCR) to combine qualitative and quantitative parasitological analyses comparing the groups under study. The weight and length of all the fish sampled were recorded and a biomass index was measured and compared along with the different fish groups. Unfortunately, the occurrence of a severe secondary bacterial infection influenced the evaluation of the efficacy of SANACORE's inclusion in fish feed. Concerning the comparison of the different diagnostic methods, the results showed that the qPCR is a more suitable method for the diagnosis of Enteromyxosis. Because of the possibility of a non-lethal technic to collect the samples (by rectal swab), it can be used as a valid screening test in a farm for newly introduced fish.A aquacultura é um sector em rápido crescimento, muito importante para a alimentação, nutrição e sustento de centenas de milhões de pessoas. A procura dos produtos do mar tem crescido e a aquacultura tem tido um papel importante de modo a satisfazer as necessidades do mercado. O aumento do número de peixes cultivados contribui também para o aumento dos problemas associados à produção, especialmente devido à elevada densidade de peixe nos locais em que é cultivado. Um dos maiores problemas sanitários que a indústria de produção de dourada (Sparus aurata) enfrenta é Enteromyxum leei, um parasita mixosporiano, que causa infeções crónicas e é responsável por baixas taxas de conversão alimentar causando dificuldades em que os peixes atinjam os tamanhos comercializados. Infelizmente, ainda não há nenhum tratamento autorizado para esta parasitose, mas recentemente um estudo experimental demonstrou bons resultados através do uso da ração funcional SANACORE®. O objetivo deste estudo é verificar a eficácia e viabilidade do alimento funcional como possível tratamento para a E. leei. O ensaio foi realizado numa importante instalação comercial de Dourada na Toscana (Itália), onde enteromixose já tinha sido diagnosticada. Para este estudo, foram testadas duas concentrações diferentes de SANACORE®, 0,5% e 0,7%, respetivamente. A prevalência e intensidade do parasita nos peixes foi avaliada através de observações microscópicas e diagnósticos moleculares (PCR) para combinar análises parasitológicas qualitativas e quantitativas comparando os grupos em estudo. O peso e comprimento dos peixes amostrados foram registados e o índice de biomassa foi medido e comparado entre os diferentes grupos de peixes. Foi esperado um aumento da biomassa e redução da intensidade do parasita nos peixes tratados com ração funcional o que poderá ser uma boa oportunidade para o aquicultor reduzir os custos associados a baixas taxas de conversão alimentar. Infelizmente, a ocorrência de uma severa segunda infeção influenciou a avaliação da eficácia da inclusão de SANACORE na ração para peixe. Relativamente à comparação dos diferentes métodos de diagnóstico, os resultados demonstraram que o qPCR é o método mais adequado para o diagnóstico de Enteromixosismo. Devido à possibilidade de uma técnica não letal para recolher as amostras (esfregaço retal), esta pode ser usada como um teste de triagem válido numa aquacultura para os peixes recém chegados.Cabral Silva, R.Gustinelli, AndreaSapientiaPierantozzi, Mirko2023-01-10T14:05:06Z2022-03-312022-03-31T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.1/18782TID:203072413enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:31:08Zoai:sapientia.ualg.pt:10400.1/18782Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:08:30.891374Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Effect of functional feed additive on symptoms reduction of enteromyxosis in gilthead seabream “sparus aurata” (field experiment)
title Effect of functional feed additive on symptoms reduction of enteromyxosis in gilthead seabream “sparus aurata” (field experiment)
spellingShingle Effect of functional feed additive on symptoms reduction of enteromyxosis in gilthead seabream “sparus aurata” (field experiment)
Pierantozzi, Mirko
Mediterranean aquaculture
Gilthead seabream
Enteromyxum leei
Functional feed
Sustainable aquaculture
Domínio/Área Científica::Ciências Naturais::Outras Ciências Naturais
title_short Effect of functional feed additive on symptoms reduction of enteromyxosis in gilthead seabream “sparus aurata” (field experiment)
title_full Effect of functional feed additive on symptoms reduction of enteromyxosis in gilthead seabream “sparus aurata” (field experiment)
title_fullStr Effect of functional feed additive on symptoms reduction of enteromyxosis in gilthead seabream “sparus aurata” (field experiment)
title_full_unstemmed Effect of functional feed additive on symptoms reduction of enteromyxosis in gilthead seabream “sparus aurata” (field experiment)
title_sort Effect of functional feed additive on symptoms reduction of enteromyxosis in gilthead seabream “sparus aurata” (field experiment)
author Pierantozzi, Mirko
author_facet Pierantozzi, Mirko
author_role author
dc.contributor.none.fl_str_mv Cabral Silva, R.
Gustinelli, Andrea
Sapientia
dc.contributor.author.fl_str_mv Pierantozzi, Mirko
dc.subject.por.fl_str_mv Mediterranean aquaculture
Gilthead seabream
Enteromyxum leei
Functional feed
Sustainable aquaculture
Domínio/Área Científica::Ciências Naturais::Outras Ciências Naturais
topic Mediterranean aquaculture
Gilthead seabream
Enteromyxum leei
Functional feed
Sustainable aquaculture
Domínio/Área Científica::Ciências Naturais::Outras Ciências Naturais
description Aquaculture is a fast-growing sector, very important for the food, nutrition, income, and livelihood of hundreds of millions of people. The demand for seafood is increasing and aquaculture plays an important role in satisfying that. The increasing number of fish farmed go at the same pace as the health and welfare problems especially because of the high density of the fish. One of the biggest sanitary problems for the gilthead seabream industry is Enteromyxum leei, a myxosporean parasite since the infection has a chronic course and is responsible for poor food conversion rate and difficulty to reach commercial size. Unfortunately, no treatments are authorized for this parasitosis, but a recent experimental study shows a good result using SANACORE® functional feed as a treatment for this parasitosis. This study aims to verify the efficacy and the feasibility of the functional feed as a possible treatment for E. leei. The trial was done in an important commercial facility of gilthead seabream in Tuscany (Italy) where enteromyxosis has been already diagnosed in the past. For this study, two different concentrations of SANACORE® are tested, respectively 0.5% and 0.7%. Prevalence and intensity of the parasite in the fish are evaluated using microscopy observation and molecular diagnosis (PCR) to combine qualitative and quantitative parasitological analyses comparing the groups under study. The weight and length of all the fish sampled were recorded and a biomass index was measured and compared along with the different fish groups. Unfortunately, the occurrence of a severe secondary bacterial infection influenced the evaluation of the efficacy of SANACORE's inclusion in fish feed. Concerning the comparison of the different diagnostic methods, the results showed that the qPCR is a more suitable method for the diagnosis of Enteromyxosis. Because of the possibility of a non-lethal technic to collect the samples (by rectal swab), it can be used as a valid screening test in a farm for newly introduced fish.
publishDate 2022
dc.date.none.fl_str_mv 2022-03-31
2022-03-31T00:00:00Z
2023-01-10T14:05:06Z
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