Response surface methodology (RSM) for optimization of Euphorbia resinifera and Euphorbia officinarum extracts with antioxidant and anti-diabetic activities
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/18990 |
Resumo: | Background: Euphorbia is one of the plants most used by herbalists and therapists in Morocco. Objectives: The aerial part of two plant samples (Euphorbia resinifera and Euphorbia officinarum) collected in Morocco was examined for the solvent effect, extraction time, and plant concentration in order to determine the best extraction conditions. Materials and Methods: To achieve this goal, a response surface methodology (RSM) using a full three-level factorial design was used to optimize the conditions for the extraction of antioxidants and a-glucosidase inhibitors. Temperature, time, and plant-to-solvent ratio (PSR) and their linear and quadratic interactions on TPC (total phenol concentration), TFC (total flavonoid concentration), DPPH (2,2-diphenyl-1-picrylhydrazyl) trapping activity, and a-glucosidase inhibiting activities were studied. Results: According to desirability functions, the optimum operating conditions to achieve a higher extraction yield of phenols and higher antioxidant and anti-diabetic activity were found by using extraction during 60 min at 30 degrees C using a PSR of 20 mg/mL, whereas a longer extraction time (270 min) was needed for E. resinifera and a higher extraction temperature (50 degrees C), with a lower PSR (10 mg/mL) for E. officinarum. Conclusion: In order to find the best conditions to extract secondary metabolites with biological activity and application in phytotherapy, the appropriate solvent generally used by populations, water in this case, should be used, but the best extraction conditions have to be found in order to enhance the pharmacological actions. |
id |
RCAP_69ed13cff86408502acbbb4aa0b6c038 |
---|---|
oai_identifier_str |
oai:sapientia.ualg.pt:10400.1/18990 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Response surface methodology (RSM) for optimization of Euphorbia resinifera and Euphorbia officinarum extracts with antioxidant and anti-diabetic activitiesa-glucosidase inhibitory activityFactorial designResponse surface methodologyTotal phenolic compoundsTotal flavonoidsBackground: Euphorbia is one of the plants most used by herbalists and therapists in Morocco. Objectives: The aerial part of two plant samples (Euphorbia resinifera and Euphorbia officinarum) collected in Morocco was examined for the solvent effect, extraction time, and plant concentration in order to determine the best extraction conditions. Materials and Methods: To achieve this goal, a response surface methodology (RSM) using a full three-level factorial design was used to optimize the conditions for the extraction of antioxidants and a-glucosidase inhibitors. Temperature, time, and plant-to-solvent ratio (PSR) and their linear and quadratic interactions on TPC (total phenol concentration), TFC (total flavonoid concentration), DPPH (2,2-diphenyl-1-picrylhydrazyl) trapping activity, and a-glucosidase inhibiting activities were studied. Results: According to desirability functions, the optimum operating conditions to achieve a higher extraction yield of phenols and higher antioxidant and anti-diabetic activity were found by using extraction during 60 min at 30 degrees C using a PSR of 20 mg/mL, whereas a longer extraction time (270 min) was needed for E. resinifera and a higher extraction temperature (50 degrees C), with a lower PSR (10 mg/mL) for E. officinarum. Conclusion: In order to find the best conditions to extract secondary metabolites with biological activity and application in phytotherapy, the appropriate solvent generally used by populations, water in this case, should be used, but the best extraction conditions have to be found in order to enhance the pharmacological actions.Wolters Kluwer Medknow PublicationsSapientiaBoutoub, OumaimaAAZZA, SmailEL-GUENDOUZ, SoukainaEl Ghadraoui, LahsenMiguel, Maria2023-02-02T13:42:08Z2022-102022-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/18990eng10.4103/pm.pm_2_220976-4062info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:31:22Zoai:sapientia.ualg.pt:10400.1/18990Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:08:40.288397Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Response surface methodology (RSM) for optimization of Euphorbia resinifera and Euphorbia officinarum extracts with antioxidant and anti-diabetic activities |
title |
Response surface methodology (RSM) for optimization of Euphorbia resinifera and Euphorbia officinarum extracts with antioxidant and anti-diabetic activities |
spellingShingle |
Response surface methodology (RSM) for optimization of Euphorbia resinifera and Euphorbia officinarum extracts with antioxidant and anti-diabetic activities Boutoub, Oumaima a-glucosidase inhibitory activity Factorial design Response surface methodology Total phenolic compounds Total flavonoids |
title_short |
Response surface methodology (RSM) for optimization of Euphorbia resinifera and Euphorbia officinarum extracts with antioxidant and anti-diabetic activities |
title_full |
Response surface methodology (RSM) for optimization of Euphorbia resinifera and Euphorbia officinarum extracts with antioxidant and anti-diabetic activities |
title_fullStr |
Response surface methodology (RSM) for optimization of Euphorbia resinifera and Euphorbia officinarum extracts with antioxidant and anti-diabetic activities |
title_full_unstemmed |
Response surface methodology (RSM) for optimization of Euphorbia resinifera and Euphorbia officinarum extracts with antioxidant and anti-diabetic activities |
title_sort |
Response surface methodology (RSM) for optimization of Euphorbia resinifera and Euphorbia officinarum extracts with antioxidant and anti-diabetic activities |
author |
Boutoub, Oumaima |
author_facet |
Boutoub, Oumaima AAZZA, Smail EL-GUENDOUZ, Soukaina El Ghadraoui, Lahsen Miguel, Maria |
author_role |
author |
author2 |
AAZZA, Smail EL-GUENDOUZ, Soukaina El Ghadraoui, Lahsen Miguel, Maria |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Boutoub, Oumaima AAZZA, Smail EL-GUENDOUZ, Soukaina El Ghadraoui, Lahsen Miguel, Maria |
dc.subject.por.fl_str_mv |
a-glucosidase inhibitory activity Factorial design Response surface methodology Total phenolic compounds Total flavonoids |
topic |
a-glucosidase inhibitory activity Factorial design Response surface methodology Total phenolic compounds Total flavonoids |
description |
Background: Euphorbia is one of the plants most used by herbalists and therapists in Morocco. Objectives: The aerial part of two plant samples (Euphorbia resinifera and Euphorbia officinarum) collected in Morocco was examined for the solvent effect, extraction time, and plant concentration in order to determine the best extraction conditions. Materials and Methods: To achieve this goal, a response surface methodology (RSM) using a full three-level factorial design was used to optimize the conditions for the extraction of antioxidants and a-glucosidase inhibitors. Temperature, time, and plant-to-solvent ratio (PSR) and their linear and quadratic interactions on TPC (total phenol concentration), TFC (total flavonoid concentration), DPPH (2,2-diphenyl-1-picrylhydrazyl) trapping activity, and a-glucosidase inhibiting activities were studied. Results: According to desirability functions, the optimum operating conditions to achieve a higher extraction yield of phenols and higher antioxidant and anti-diabetic activity were found by using extraction during 60 min at 30 degrees C using a PSR of 20 mg/mL, whereas a longer extraction time (270 min) was needed for E. resinifera and a higher extraction temperature (50 degrees C), with a lower PSR (10 mg/mL) for E. officinarum. Conclusion: In order to find the best conditions to extract secondary metabolites with biological activity and application in phytotherapy, the appropriate solvent generally used by populations, water in this case, should be used, but the best extraction conditions have to be found in order to enhance the pharmacological actions. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-10 2022-10-01T00:00:00Z 2023-02-02T13:42:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/18990 |
url |
http://hdl.handle.net/10400.1/18990 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.4103/pm.pm_2_22 0976-4062 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wolters Kluwer Medknow Publications |
publisher.none.fl_str_mv |
Wolters Kluwer Medknow Publications |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799133333382234112 |