Evaluation of Opuntia spp. bioactive products as promising natural chemotherapeutical agents- an in vitro approach

Detalhes bibliográficos
Autor(a) principal: Poejo, Joana Margarida de Andrade
Data de Publicação: 2011
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/6289
Resumo: Dissertation to obtain a Master Degree in Biotechnology
id RCAP_69f8a101784837128c6146ad7f5e33e4
oai_identifier_str oai:run.unl.pt:10362/6289
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Evaluation of Opuntia spp. bioactive products as promising natural chemotherapeutical agents- an in vitro approachColon cancerPolyphenolsOpuntia sppAntiproliferative effectChemoresistance.Dissertation to obtain a Master Degree in BiotechnologyRecently, prevention of cancer through dietary intervention has received an increasing interest. In particular, dietary polyphenols mainly found in fruits and vegetables have become very attractive as chemopreventive and therapeutic agents. The main aim of this work was to investigate the potential activity of Portuguese Opuntia spp. on colon cancer therapy, as this fruits have been reported to be rich sources of bioactive compounds. Opuntia spp. fruits were collected in different sites of Portugal, namely Tramagal, Beja, Marvão Sines and Quarteira and processed in order to obtain fruit juices, polyphenol-rich concentrates and residues extracts from fruit juices production. All Opuntia products were investigated for antiproliferative activity of human colon cancer cells (HT29). Opuntia juices from Tramagal, Sines and Quarteira were the most effective on cancer cell growth inhibition due to the highest content in total polyphenol content and betalains. The most promising extracts were derived from Quarteira, Sines and Beja fruit juices residues using conventional solvent extraction, adsorption technology and pressurized liquid extraction, respectively. These extracts showed high antiproliferative effect inducing cell cycle arrest in different checkpoints. Additionally, the same extracts showed to induce ROS production in cancer cells, playing an important role in activation of pro-apoptic cell death mechanisms. The capacity of natural extracts in reverting chemoresistance of tumor cells was evaluated on a drug-resistant HT29 cell culture. The product derived from Beja residue using pressurized liquid extraction with 60%CO2 and 40%EtOH showed to increase cells sensitivity to the drug. Opuntia spp. fruits reveled to be promising natural sources for the production of functional products as well as for the development of novel chemotherapeutic agents.Faculdade de Ciências e TecnologiaSerra, AnaDuarte, CatarinaRUNPoejo, Joana Margarida de Andrade2011-12-12T11:17:48Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/6289enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:37:32Zoai:run.unl.pt:10362/6289Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:16:49.615655Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Evaluation of Opuntia spp. bioactive products as promising natural chemotherapeutical agents- an in vitro approach
title Evaluation of Opuntia spp. bioactive products as promising natural chemotherapeutical agents- an in vitro approach
spellingShingle Evaluation of Opuntia spp. bioactive products as promising natural chemotherapeutical agents- an in vitro approach
Poejo, Joana Margarida de Andrade
Colon cancer
Polyphenols
Opuntia spp
Antiproliferative effect
Chemoresistance.
title_short Evaluation of Opuntia spp. bioactive products as promising natural chemotherapeutical agents- an in vitro approach
title_full Evaluation of Opuntia spp. bioactive products as promising natural chemotherapeutical agents- an in vitro approach
title_fullStr Evaluation of Opuntia spp. bioactive products as promising natural chemotherapeutical agents- an in vitro approach
title_full_unstemmed Evaluation of Opuntia spp. bioactive products as promising natural chemotherapeutical agents- an in vitro approach
title_sort Evaluation of Opuntia spp. bioactive products as promising natural chemotherapeutical agents- an in vitro approach
author Poejo, Joana Margarida de Andrade
author_facet Poejo, Joana Margarida de Andrade
author_role author
dc.contributor.none.fl_str_mv Serra, Ana
Duarte, Catarina
RUN
dc.contributor.author.fl_str_mv Poejo, Joana Margarida de Andrade
dc.subject.por.fl_str_mv Colon cancer
Polyphenols
Opuntia spp
Antiproliferative effect
Chemoresistance.
topic Colon cancer
Polyphenols
Opuntia spp
Antiproliferative effect
Chemoresistance.
description Dissertation to obtain a Master Degree in Biotechnology
publishDate 2011
dc.date.none.fl_str_mv 2011-12-12T11:17:48Z
2011
2011-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/6289
url http://hdl.handle.net/10362/6289
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799137817432948736

Registros relacionados