Algorithm to assess the presence of Aspergillus fumigatus resistant strains: The case of Norwegian sawmills

Detalhes bibliográficos
Autor(a) principal: Viegas, Carla
Data de Publicação: 2020
Outros Autores: Almeida, Beatriz, Aranha Caetano, Liliana, Afanou, Anani, Straumfors, Anne, Veríssimo, Cristina, Gonçalves, Paulo, Sabino, Raquel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/7631
Resumo: Association between selection pressure caused by the use of azole fungicides in sawmills and the development of fungal resistance has been described. The aim of this study was to implement an algorithm to assess the presence of Aspergillus section Fumigati resistant strains in sawmills. Eighty-six full-shift inhalable dust samples were collected from eleven industrial sawmills in Norway. Different culture media were used and molecular identification to species level in Aspergillus section Fumigati was done by calmodulin sequencing and TR34/L98H and TR46/Y121F/T289A mutations were screened by real-time PCR assay and confirmed by cyp51A sequencing. Six Fumigati isolates were identified as A. fumigatus sensu stricto and two of these grew on azole-supplemented media and were further analyzed by real-time PCR. One was confirmed to be a TR34/L98H mutant. The obtained results reinforce the need to assess the presence of A. fumigatus sensu stricto resistant isolates at other workplaces with fungicide pressure.
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spelling Algorithm to assess the presence of Aspergillus fumigatus resistant strains: The case of Norwegian sawmillsAspergillus fumigatus Resistant IsolatesSection FumigatiCross-ResistanceFungicide PressureOccupational ExposureInfecções Sistémicas e ZoonosesAssociation between selection pressure caused by the use of azole fungicides in sawmills and the development of fungal resistance has been described. The aim of this study was to implement an algorithm to assess the presence of Aspergillus section Fumigati resistant strains in sawmills. Eighty-six full-shift inhalable dust samples were collected from eleven industrial sawmills in Norway. Different culture media were used and molecular identification to species level in Aspergillus section Fumigati was done by calmodulin sequencing and TR34/L98H and TR46/Y121F/T289A mutations were screened by real-time PCR assay and confirmed by cyp51A sequencing. Six Fumigati isolates were identified as A. fumigatus sensu stricto and two of these grew on azole-supplemented media and were further analyzed by real-time PCR. One was confirmed to be a TR34/L98H mutant. The obtained results reinforce the need to assess the presence of A. fumigatus sensu stricto resistant isolates at other workplaces with fungicide pressure.This work was supported by the Research council of Norway [218232/H20]; Instituto Politécnico de Lisboa [IPL/ 2018/WasteFRPD_ESTeSL]; Fundação para Ciência e Tecnologia [02/SAICT/2016 – Project nº 23222]. H&TRC authors gratefully acknowledge the FCT/MCTES national support through the UIDB/05608/2020 and UIDP/05608/ 2020.Taylor and Francis/ International Federation of Environmental HealthRepositório Científico do Instituto Nacional de SaúdeViegas, CarlaAlmeida, BeatrizAranha Caetano, LilianaAfanou, AnaniStraumfors, AnneVeríssimo, CristinaGonçalves, PauloSabino, Raquel2022-08-19T00:30:22Z2020-08-192020-08-19T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/7631engInt J Environ Health Res. 2020 Aug 19;1-9. doi: 10.1080/09603123.2020.1810210. Online ahead of print0960-312310.1080/09603123.2020.1810210info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:07Zoai:repositorio.insa.pt:10400.18/7631Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:16.133710Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Algorithm to assess the presence of Aspergillus fumigatus resistant strains: The case of Norwegian sawmills
title Algorithm to assess the presence of Aspergillus fumigatus resistant strains: The case of Norwegian sawmills
spellingShingle Algorithm to assess the presence of Aspergillus fumigatus resistant strains: The case of Norwegian sawmills
Viegas, Carla
Aspergillus fumigatus Resistant Isolates
Section Fumigati
Cross-Resistance
Fungicide Pressure
Occupational Exposure
Infecções Sistémicas e Zoonoses
title_short Algorithm to assess the presence of Aspergillus fumigatus resistant strains: The case of Norwegian sawmills
title_full Algorithm to assess the presence of Aspergillus fumigatus resistant strains: The case of Norwegian sawmills
title_fullStr Algorithm to assess the presence of Aspergillus fumigatus resistant strains: The case of Norwegian sawmills
title_full_unstemmed Algorithm to assess the presence of Aspergillus fumigatus resistant strains: The case of Norwegian sawmills
title_sort Algorithm to assess the presence of Aspergillus fumigatus resistant strains: The case of Norwegian sawmills
author Viegas, Carla
author_facet Viegas, Carla
Almeida, Beatriz
Aranha Caetano, Liliana
Afanou, Anani
Straumfors, Anne
Veríssimo, Cristina
Gonçalves, Paulo
Sabino, Raquel
author_role author
author2 Almeida, Beatriz
Aranha Caetano, Liliana
Afanou, Anani
Straumfors, Anne
Veríssimo, Cristina
Gonçalves, Paulo
Sabino, Raquel
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Viegas, Carla
Almeida, Beatriz
Aranha Caetano, Liliana
Afanou, Anani
Straumfors, Anne
Veríssimo, Cristina
Gonçalves, Paulo
Sabino, Raquel
dc.subject.por.fl_str_mv Aspergillus fumigatus Resistant Isolates
Section Fumigati
Cross-Resistance
Fungicide Pressure
Occupational Exposure
Infecções Sistémicas e Zoonoses
topic Aspergillus fumigatus Resistant Isolates
Section Fumigati
Cross-Resistance
Fungicide Pressure
Occupational Exposure
Infecções Sistémicas e Zoonoses
description Association between selection pressure caused by the use of azole fungicides in sawmills and the development of fungal resistance has been described. The aim of this study was to implement an algorithm to assess the presence of Aspergillus section Fumigati resistant strains in sawmills. Eighty-six full-shift inhalable dust samples were collected from eleven industrial sawmills in Norway. Different culture media were used and molecular identification to species level in Aspergillus section Fumigati was done by calmodulin sequencing and TR34/L98H and TR46/Y121F/T289A mutations were screened by real-time PCR assay and confirmed by cyp51A sequencing. Six Fumigati isolates were identified as A. fumigatus sensu stricto and two of these grew on azole-supplemented media and were further analyzed by real-time PCR. One was confirmed to be a TR34/L98H mutant. The obtained results reinforce the need to assess the presence of A. fumigatus sensu stricto resistant isolates at other workplaces with fungicide pressure.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-19
2020-08-19T00:00:00Z
2022-08-19T00:30:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/7631
url http://hdl.handle.net/10400.18/7631
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Int J Environ Health Res. 2020 Aug 19;1-9. doi: 10.1080/09603123.2020.1810210. Online ahead of print
0960-3123
10.1080/09603123.2020.1810210
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor and Francis/ International Federation of Environmental Health
publisher.none.fl_str_mv Taylor and Francis/ International Federation of Environmental Health
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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