Suppression by allogeneic-specific regulatory T cells is dependent on the degree of HLA compatibility

Detalhes bibliográficos
Autor(a) principal: Bianchi, Joana
Data de Publicação: 2021
Outros Autores: Vieira, Ana I. S., Ligeiro, Dário, Azevedo, Rita I., Lacerda, João
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/48183
Resumo: Copyright © 2021 The Authors. This article is distributed under the terms of the CC BY 4.0 Unported license.
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spelling Suppression by allogeneic-specific regulatory T cells is dependent on the degree of HLA compatibilityCopyright © 2021 The Authors. This article is distributed under the terms of the CC BY 4.0 Unported license.Regulatory T cell (Treg) infusion for graft-versus-host disease treatment has been increasingly investigated. However, polyclonal Treg may suppress the desired graft-versus-leukemia effect. Although allogeneic-specific (allo-specific) Treg may provide a more-targeted graft-versus-host disease treatment, there is the need to develop easily translatable expansion protocols and to better characterize their specificity and mechanisms of suppression. In this article, we provide a robust protocol for human allo-specific Treg expansion and characterize their phenotype, potency, and specificity of suppression by testing different expansion conditions and suppression assay milieus. We found that higher concentrations of IL-2 during expansion with allogeneic APC yielded allo-specific Treg that were more-potent suppressors and displayed a more activated phenotype. Although responses to the same APC present during expansion were the most suppressed, responses to third-party APC partially matched to the expansion APC were still significantly more suppressed than responses to fully mismatched APC. Furthermore, suppression of responses to the expansion APC was strictly contact dependent, whereas suppression of responses to mismatched APC was partially independent of contact. Finally, distinct subsets in fresh and expanded Treg could be described using multidimensional visualization techniques. We propose that allo-specific Treg are HLA specific and that the mechanisms of suppression elicited depend on their compatibility with the stimulators.This work was supported by Fundação para a Ciência e Tecnologia, Portugal, under the Harvard Medical School–Portugal Program Project Induction of Immune Tolerance in Human Allogeneic Hematopoietic Stem Cell Transplantation (HMSP-ICT/0001/2011) and Gilead Sciences, under Programa Gilead GÉNESE (FPJ001262). J.B. received a Ph.D. grant from Fundação para a Ciência e Tecnologia (PD/BD/105769/2014).The American Association of Immunologists, Inc.Repositório da Universidade de LisboaBianchi, JoanaVieira, Ana I. S.Ligeiro, DárioAzevedo, Rita I.Lacerda, João2021-05-26T13:31:15Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/48183engImmunohorizons. 2021 May 12;5(5):307-32110.4049/immunohorizons.21000212573-7732info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:51:24Zoai:repositorio.ul.pt:10451/48183Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:00:04.660969Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Suppression by allogeneic-specific regulatory T cells is dependent on the degree of HLA compatibility
title Suppression by allogeneic-specific regulatory T cells is dependent on the degree of HLA compatibility
spellingShingle Suppression by allogeneic-specific regulatory T cells is dependent on the degree of HLA compatibility
Bianchi, Joana
title_short Suppression by allogeneic-specific regulatory T cells is dependent on the degree of HLA compatibility
title_full Suppression by allogeneic-specific regulatory T cells is dependent on the degree of HLA compatibility
title_fullStr Suppression by allogeneic-specific regulatory T cells is dependent on the degree of HLA compatibility
title_full_unstemmed Suppression by allogeneic-specific regulatory T cells is dependent on the degree of HLA compatibility
title_sort Suppression by allogeneic-specific regulatory T cells is dependent on the degree of HLA compatibility
author Bianchi, Joana
author_facet Bianchi, Joana
Vieira, Ana I. S.
Ligeiro, Dário
Azevedo, Rita I.
Lacerda, João
author_role author
author2 Vieira, Ana I. S.
Ligeiro, Dário
Azevedo, Rita I.
Lacerda, João
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Bianchi, Joana
Vieira, Ana I. S.
Ligeiro, Dário
Azevedo, Rita I.
Lacerda, João
description Copyright © 2021 The Authors. This article is distributed under the terms of the CC BY 4.0 Unported license.
publishDate 2021
dc.date.none.fl_str_mv 2021-05-26T13:31:15Z
2021
2021-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/48183
url http://hdl.handle.net/10451/48183
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Immunohorizons. 2021 May 12;5(5):307-321
10.4049/immunohorizons.2100021
2573-7732
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The American Association of Immunologists, Inc.
publisher.none.fl_str_mv The American Association of Immunologists, Inc.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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