Siderophore production by Bacillus megaterium : effect of growth-phase and cultural conditions
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/32124 |
Resumo: | Siderophore production by Bacillus megaterium was detected, in an iron-deficient culture medium, during the exponential growth phase, prior to the sporulation, in the presence of glucose; these results suggested that the onset of siderophore production did not require glucose depletion and was not related with the sporulation. The siderophore production by B. megaterium was affected by the carbon source used. The growth on glycerol promoted the very high siderophore production (1,182 μmol g−1 dry weight biomass); the opposite effect was observed in the presence of mannose (251 μmol g−1 dry weight biomass). The growth in the presence of fructose, galactose, glucose, lactose, maltose or sucrose, originated similar concentrations of siderophore (546–842 μmol g−1 dry weight biomass). Aeration had a positive effect on the production of siderophore. Incubation of B. megaterium under static conditions delayed and reduced the growth and the production of siderophore, compared with the incubation in stirred conditions. |
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Siderophore production by Bacillus megaterium : effect of growth-phase and cultural conditionsBacillus megateriumCarbon sourceEnvironmental-friendly chelating agentsIron chelatorSiderophore productionScience & TechnologySiderophore production by Bacillus megaterium was detected, in an iron-deficient culture medium, during the exponential growth phase, prior to the sporulation, in the presence of glucose; these results suggested that the onset of siderophore production did not require glucose depletion and was not related with the sporulation. The siderophore production by B. megaterium was affected by the carbon source used. The growth on glycerol promoted the very high siderophore production (1,182 μmol g−1 dry weight biomass); the opposite effect was observed in the presence of mannose (251 μmol g−1 dry weight biomass). The growth in the presence of fructose, galactose, glucose, lactose, maltose or sucrose, originated similar concentrations of siderophore (546–842 μmol g−1 dry weight biomass). Aeration had a positive effect on the production of siderophore. Incubation of B. megaterium under static conditions delayed and reduced the growth and the production of siderophore, compared with the incubation in stirred conditions.The authors thank Porto University/Totta Bank for their financial support through the project "Microbiological production of chelating agents" (Ref: 180). The authors also thank the Fundacao para a Ciencia e a Tecnologia (FCT) through the Portuguese Government for their financial support of this work through the grants Strategic project-LA23/2013-2014 (IBB) and PEST-C/EQB/LA0006/2011 (REQUIMTE). Manuela D. Machado gratefully acknowledges the postdoctoral (SFRH/BPD/72816/2010) grant from FCT.Springer VerlagUniversidade do MinhoSantos, S.Neto, F. F. I.Machado, Manuela D.Soares, Helena M. V. M.Soares, Eduardo V.20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/32124engSantos, S.; Neto, F. F. I.; Machado, Manuela D.; Soares, H. M. V. M.; Soares, Eduardo V., Siderophore production by Bacillus megaterium: effect of growth-phase and cultural conditions. Applied Biochemistry and Biotechnology, 172(1), 549-560, 20140273-22890273-228910.1007/s12010-013-0562-y24101562http://link.springer.com/journal/12010info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:09:14Zoai:repositorium.sdum.uminho.pt:1822/32124Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:00:35.782366Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Siderophore production by Bacillus megaterium : effect of growth-phase and cultural conditions |
title |
Siderophore production by Bacillus megaterium : effect of growth-phase and cultural conditions |
spellingShingle |
Siderophore production by Bacillus megaterium : effect of growth-phase and cultural conditions Santos, S. Bacillus megaterium Carbon source Environmental-friendly chelating agents Iron chelator Siderophore production Science & Technology |
title_short |
Siderophore production by Bacillus megaterium : effect of growth-phase and cultural conditions |
title_full |
Siderophore production by Bacillus megaterium : effect of growth-phase and cultural conditions |
title_fullStr |
Siderophore production by Bacillus megaterium : effect of growth-phase and cultural conditions |
title_full_unstemmed |
Siderophore production by Bacillus megaterium : effect of growth-phase and cultural conditions |
title_sort |
Siderophore production by Bacillus megaterium : effect of growth-phase and cultural conditions |
author |
Santos, S. |
author_facet |
Santos, S. Neto, F. F. I. Machado, Manuela D. Soares, Helena M. V. M. Soares, Eduardo V. |
author_role |
author |
author2 |
Neto, F. F. I. Machado, Manuela D. Soares, Helena M. V. M. Soares, Eduardo V. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Santos, S. Neto, F. F. I. Machado, Manuela D. Soares, Helena M. V. M. Soares, Eduardo V. |
dc.subject.por.fl_str_mv |
Bacillus megaterium Carbon source Environmental-friendly chelating agents Iron chelator Siderophore production Science & Technology |
topic |
Bacillus megaterium Carbon source Environmental-friendly chelating agents Iron chelator Siderophore production Science & Technology |
description |
Siderophore production by Bacillus megaterium was detected, in an iron-deficient culture medium, during the exponential growth phase, prior to the sporulation, in the presence of glucose; these results suggested that the onset of siderophore production did not require glucose depletion and was not related with the sporulation. The siderophore production by B. megaterium was affected by the carbon source used. The growth on glycerol promoted the very high siderophore production (1,182 μmol g−1 dry weight biomass); the opposite effect was observed in the presence of mannose (251 μmol g−1 dry weight biomass). The growth in the presence of fructose, galactose, glucose, lactose, maltose or sucrose, originated similar concentrations of siderophore (546–842 μmol g−1 dry weight biomass). Aeration had a positive effect on the production of siderophore. Incubation of B. megaterium under static conditions delayed and reduced the growth and the production of siderophore, compared with the incubation in stirred conditions. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/32124 |
url |
http://hdl.handle.net/1822/32124 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Santos, S.; Neto, F. F. I.; Machado, Manuela D.; Soares, H. M. V. M.; Soares, Eduardo V., Siderophore production by Bacillus megaterium: effect of growth-phase and cultural conditions. Applied Biochemistry and Biotechnology, 172(1), 549-560, 2014 0273-2289 0273-2289 10.1007/s12010-013-0562-y 24101562 http://link.springer.com/journal/12010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer Verlag |
publisher.none.fl_str_mv |
Springer Verlag |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132401834655744 |