Occurrence of ESBL-producing Escherichia coli in soils subjected to livestock grazing in Azores archipelago : an environment-health pollution issue?
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.3/6034 |
Resumo: | Antibiotics are successful drugs used in human and animal therapy; however, they must be considered as environmental pollutants. This study aims to isolate and characterize the extended-spectrum β-lactamase (ESBL) producing Escherichia coli soil from Azores Archipelago subjected to livestock agricultural practices. Twenty-four soil samples were collected from three different pasture systems with different number of cattle heads, and from a control site. Antibiotic susceptibility method was performed by Kirby-Bauer disk diffusion method against 16 antibiotics, and the presence of genes encoding lactamases, antimicrobial resistance genes, virulence factors, and phylogenetic groups was determined by polymerase chain reaction (PCR). Nine ESBLs were recovered from the three grazing sites, and all isolates presented the beta-lactamase genes blaCTX-M-3 and blaSHV. E. coli isolates were resistance to tetracycline and streptomycin and harbored the tetB, strA, and strB genes. One isolate also showed resistance to sulfonamides, and the genes sul1 and sul2 were detected. The isolates were grouped into the following phylogenic groups: B1 (n = 6), D (n = 2), and A (n = 1). The presence of antibiotics and resistance genes in soils may be the source to the development of antimicrobial resistance, which may have negative consequences in human and animal health. |
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Occurrence of ESBL-producing Escherichia coli in soils subjected to livestock grazing in Azores archipelago : an environment-health pollution issue?CTX-MEscherichia coliEnvironmental PollutionExtended-spectrum beta-lactamaseLivestockSoilAntibiotics are successful drugs used in human and animal therapy; however, they must be considered as environmental pollutants. This study aims to isolate and characterize the extended-spectrum β-lactamase (ESBL) producing Escherichia coli soil from Azores Archipelago subjected to livestock agricultural practices. Twenty-four soil samples were collected from three different pasture systems with different number of cattle heads, and from a control site. Antibiotic susceptibility method was performed by Kirby-Bauer disk diffusion method against 16 antibiotics, and the presence of genes encoding lactamases, antimicrobial resistance genes, virulence factors, and phylogenetic groups was determined by polymerase chain reaction (PCR). Nine ESBLs were recovered from the three grazing sites, and all isolates presented the beta-lactamase genes blaCTX-M-3 and blaSHV. E. coli isolates were resistance to tetracycline and streptomycin and harbored the tetB, strA, and strB genes. One isolate also showed resistance to sulfonamides, and the genes sul1 and sul2 were detected. The isolates were grouped into the following phylogenic groups: B1 (n = 6), D (n = 2), and A (n = 1). The presence of antibiotics and resistance genes in soils may be the source to the development of antimicrobial resistance, which may have negative consequences in human and animal health.This work was supported by the Associate Laboratory for Green Chemistry- LAQV which is financed by national funds from FCT/MCTES (UID/QUI/50006/2020). Vanessa Silva received a PhD grant from FCT (Fundação para a Ciência e Tecnologia) (SFRH/BD/137947/2018).Springer - Spanish Society for MicrobiologyRepositório da Universidade dos AçoresSilva, VanessaPeixoto, FernandoParelho, Carolina Paula Furtado de MedeirosGarcia, PatríciaRodrigues, ArmindoSilva, AdrianaCarvalho, IsabelPereira, José EduardoIgrejas, GilbertoPoeta, Patrícia Alexandra Curado Quintas Dinis2021-09-07T12:19:13Z2020-062020-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.3/6034engSilva, V., Peixoto, F., Parelho, C., Garcia, P., Rodrigues, A., Carvalho, I., Pereira, J.E., Igrejas, G. & Poeta P. (2020). Occurrence of ESBL-producing Escherichia coli in soils subjected to livestock grazing in Azores archipelago. An environment-health pollution issue? "International Microbiology", 23(4), 619-624. DOI:10.1007/s10123-020-00134-01139-670910.1007/s10123-020-00134-01618-190532514644info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-12-20T14:34:23Zoai:repositorio.uac.pt:10400.3/6034Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:28:10.542754Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Occurrence of ESBL-producing Escherichia coli in soils subjected to livestock grazing in Azores archipelago : an environment-health pollution issue? |
title |
Occurrence of ESBL-producing Escherichia coli in soils subjected to livestock grazing in Azores archipelago : an environment-health pollution issue? |
spellingShingle |
Occurrence of ESBL-producing Escherichia coli in soils subjected to livestock grazing in Azores archipelago : an environment-health pollution issue? Silva, Vanessa CTX-M Escherichia coli Environmental Pollution Extended-spectrum beta-lactamase Livestock Soil |
title_short |
Occurrence of ESBL-producing Escherichia coli in soils subjected to livestock grazing in Azores archipelago : an environment-health pollution issue? |
title_full |
Occurrence of ESBL-producing Escherichia coli in soils subjected to livestock grazing in Azores archipelago : an environment-health pollution issue? |
title_fullStr |
Occurrence of ESBL-producing Escherichia coli in soils subjected to livestock grazing in Azores archipelago : an environment-health pollution issue? |
title_full_unstemmed |
Occurrence of ESBL-producing Escherichia coli in soils subjected to livestock grazing in Azores archipelago : an environment-health pollution issue? |
title_sort |
Occurrence of ESBL-producing Escherichia coli in soils subjected to livestock grazing in Azores archipelago : an environment-health pollution issue? |
author |
Silva, Vanessa |
author_facet |
Silva, Vanessa Peixoto, Fernando Parelho, Carolina Paula Furtado de Medeiros Garcia, Patrícia Rodrigues, Armindo Silva, Adriana Carvalho, Isabel Pereira, José Eduardo Igrejas, Gilberto Poeta, Patrícia Alexandra Curado Quintas Dinis |
author_role |
author |
author2 |
Peixoto, Fernando Parelho, Carolina Paula Furtado de Medeiros Garcia, Patrícia Rodrigues, Armindo Silva, Adriana Carvalho, Isabel Pereira, José Eduardo Igrejas, Gilberto Poeta, Patrícia Alexandra Curado Quintas Dinis |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade dos Açores |
dc.contributor.author.fl_str_mv |
Silva, Vanessa Peixoto, Fernando Parelho, Carolina Paula Furtado de Medeiros Garcia, Patrícia Rodrigues, Armindo Silva, Adriana Carvalho, Isabel Pereira, José Eduardo Igrejas, Gilberto Poeta, Patrícia Alexandra Curado Quintas Dinis |
dc.subject.por.fl_str_mv |
CTX-M Escherichia coli Environmental Pollution Extended-spectrum beta-lactamase Livestock Soil |
topic |
CTX-M Escherichia coli Environmental Pollution Extended-spectrum beta-lactamase Livestock Soil |
description |
Antibiotics are successful drugs used in human and animal therapy; however, they must be considered as environmental pollutants. This study aims to isolate and characterize the extended-spectrum β-lactamase (ESBL) producing Escherichia coli soil from Azores Archipelago subjected to livestock agricultural practices. Twenty-four soil samples were collected from three different pasture systems with different number of cattle heads, and from a control site. Antibiotic susceptibility method was performed by Kirby-Bauer disk diffusion method against 16 antibiotics, and the presence of genes encoding lactamases, antimicrobial resistance genes, virulence factors, and phylogenetic groups was determined by polymerase chain reaction (PCR). Nine ESBLs were recovered from the three grazing sites, and all isolates presented the beta-lactamase genes blaCTX-M-3 and blaSHV. E. coli isolates were resistance to tetracycline and streptomycin and harbored the tetB, strA, and strB genes. One isolate also showed resistance to sulfonamides, and the genes sul1 and sul2 were detected. The isolates were grouped into the following phylogenic groups: B1 (n = 6), D (n = 2), and A (n = 1). The presence of antibiotics and resistance genes in soils may be the source to the development of antimicrobial resistance, which may have negative consequences in human and animal health. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-06 2020-06-01T00:00:00Z 2021-09-07T12:19:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.3/6034 |
url |
http://hdl.handle.net/10400.3/6034 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Silva, V., Peixoto, F., Parelho, C., Garcia, P., Rodrigues, A., Carvalho, I., Pereira, J.E., Igrejas, G. & Poeta P. (2020). Occurrence of ESBL-producing Escherichia coli in soils subjected to livestock grazing in Azores archipelago. An environment-health pollution issue? "International Microbiology", 23(4), 619-624. DOI:10.1007/s10123-020-00134-0 1139-6709 10.1007/s10123-020-00134-0 1618-1905 32514644 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer - Spanish Society for Microbiology |
publisher.none.fl_str_mv |
Springer - Spanish Society for Microbiology |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799130738839257088 |