Purification of polysaccharides from a biofilm matrix by selective precipitation of proteins

Detalhes bibliográficos
Autor(a) principal: Oliveira, Rosário
Data de Publicação: 1999
Outros Autores: Marques, F., Azeredo, Joana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/1383
Resumo: The objective was to obtain the polysaccharide fraction presented in the extracted polymeric matrix of a biofilm by differential precipitation of the protein content. This was tried by pH adjustment and by addition of trichloroacetic acid (TCA). The best yield of protein precipitation was with 20% (w/v) TCA, with more than 97% of the protein removed and without affecting the amount of polysaccharides in solution.
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spelling Purification of polysaccharides from a biofilm matrix by selective precipitation of proteinsDifferential precipitationPolysaccharidesProteinsScience & TechnologyThe objective was to obtain the polysaccharide fraction presented in the extracted polymeric matrix of a biofilm by differential precipitation of the protein content. This was tried by pH adjustment and by addition of trichloroacetic acid (TCA). The best yield of protein precipitation was with 20% (w/v) TCA, with more than 97% of the protein removed and without affecting the amount of polysaccharides in solution.KluwerUniversidade do MinhoOliveira, RosárioMarques, F.Azeredo, Joana19991999-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/1383eng"Biotechnology techniques" ISSN 0951-208X. 13:6 (1999) 391-393.0951-208X10.1023/A:1008954301470info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:43:24Zoai:repositorium.sdum.uminho.pt:1822/1383Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:40:53.024186Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Purification of polysaccharides from a biofilm matrix by selective precipitation of proteins
title Purification of polysaccharides from a biofilm matrix by selective precipitation of proteins
spellingShingle Purification of polysaccharides from a biofilm matrix by selective precipitation of proteins
Oliveira, Rosário
Differential precipitation
Polysaccharides
Proteins
Science & Technology
title_short Purification of polysaccharides from a biofilm matrix by selective precipitation of proteins
title_full Purification of polysaccharides from a biofilm matrix by selective precipitation of proteins
title_fullStr Purification of polysaccharides from a biofilm matrix by selective precipitation of proteins
title_full_unstemmed Purification of polysaccharides from a biofilm matrix by selective precipitation of proteins
title_sort Purification of polysaccharides from a biofilm matrix by selective precipitation of proteins
author Oliveira, Rosário
author_facet Oliveira, Rosário
Marques, F.
Azeredo, Joana
author_role author
author2 Marques, F.
Azeredo, Joana
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Oliveira, Rosário
Marques, F.
Azeredo, Joana
dc.subject.por.fl_str_mv Differential precipitation
Polysaccharides
Proteins
Science & Technology
topic Differential precipitation
Polysaccharides
Proteins
Science & Technology
description The objective was to obtain the polysaccharide fraction presented in the extracted polymeric matrix of a biofilm by differential precipitation of the protein content. This was tried by pH adjustment and by addition of trichloroacetic acid (TCA). The best yield of protein precipitation was with 20% (w/v) TCA, with more than 97% of the protein removed and without affecting the amount of polysaccharides in solution.
publishDate 1999
dc.date.none.fl_str_mv 1999
1999-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/1383
url http://hdl.handle.net/1822/1383
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Biotechnology techniques" ISSN 0951-208X. 13:6 (1999) 391-393.
0951-208X
10.1023/A:1008954301470
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Kluwer
publisher.none.fl_str_mv Kluwer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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