Electrospun nanofibrous poly (Lactic Acid)/Titanium dioxide nanocomposite membranes for cutaneous scar minimization
| Autor(a) principal: | |
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| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/1822/62736 |
Resumo: | The animal study was reviewed and approved by 10/2015-CEUA/ICT/CJSC-UNESP. |
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Electrospun nanofibrous poly (Lactic Acid)/Titanium dioxide nanocomposite membranes for cutaneous scar minimizationPLAnanocompositeselectrospinningcutaneous scarringgene expressionin vivoEngenharia e Tecnologia::Engenharia dos MateriaisScience & TechnologyThe animal study was reviewed and approved by 10/2015-CEUA/ICT/CJSC-UNESP.Poly (lactic acid) (PLA) has been increasingly used in cutaneous tissue engineering due to its low cost, ease of handling, biodegradability, and biocompatibility, as well as its ability to form composites. However, these polymers possess a structure with nanoporous that mimic the cellular environment. In this study, nanocomposites are prepared using PLA and titanium dioxide (TiO2) (10 and 35%—w/w) nanoparticles that also function as an active anti-scarring agent. The nanocomposites were prepared using an electrospinning technique. Three different solutions were prepared as follows: PLA, 10% PLA/TiO2, and 35% PLA/TiO2 (w/w%). Electrospun PLA and PLA/TiO2 nanocomposites were characterized morphologically, structurally, and chemically using electron scanning microscopy, transmission electron microscopy, goniometry, and X-ray diffraction. L929 fibroblast cells were used for in vitro tests. The cytotoxic effect was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. Versicam (VCAN), biglicam (BIG), interleukin-6 (IL6), interleukin-10 (IL-10), and type-1 collagen (COL1A1) genes were evaluated by RT-qPCR. In vivo tests using Wistar rats were conducted for up to 15 days. Nanofibrous fibers were obtained for all groups that did not contain residual solvents. No cytotoxic effects were observed for up to 168 h. The genes expressed showed the highest values of versican and collagen-1 (p < 0.05) for PLA/TiO2 nanocomposite scaffolds when compared to the control group (cells). Histological images showed that PLA at 10 and 35% w/w led to a discrete inflammatory infiltration and expression of many newly formed vessels, indicating increased metabolic activity of this tissue. To summarize, this study supported the potential of PLA/TiO2 nanocomposites ability to reduce cutaneous scarring in scaffolds.This work was supported by the National Council for Scientificand Technological Development (CNPq, #303752/2017-3 and#404683/2018-5 to AL and #304133/2017-5 and #424163/2016-0 to FM). AZ acknowledges financial support of the FCT throughUID/CTM/00264/2019 and Investigator FCT Research contract(IF/00071/2015) and the project PTDC/CTM-TEX/28295/2017 financed by FCT, FEDER, and POCI.Frontiers MediaUniversidade do MinhoMarsi, Teresa C. O.Ricci, RitchelliToniato, Tatiane V.Vasconcellos, Luana M. R.Elias, Conceição de Maria VazSilva, Andre D. R.Furtado, Andre S. A.Magalhães, Leila S. S. M.Silva-Filho, Edson C.Marciano, Fernanda R.Zille, AndreaWebster, Thomas J.Lobo, Anderson O.20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/62736engMarsi TCO, Ricci R, Toniato TV,Vasconcellos LMR, Elias CdMV,Silva ADR, Furtado ASA,Magalhães LSSM, Silva-Filho EC,Marciano FR, Zille A, Webster TJ andLobo AO (2019) ElectrospunNanofibrous Poly (LacticAcid)/Titanium DioxideNanocomposite Membranes forCutaneous Scar Minimization.Front. Bioeng. Biotechnol. 7:421.doi: 10.3389/fbioe.2019.004212296-418510.3389/fbioe.2019.00421info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:17:16Zoai:repositorium.sdum.uminho.pt:1822/62736Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:09:52.192171Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Electrospun nanofibrous poly (Lactic Acid)/Titanium dioxide nanocomposite membranes for cutaneous scar minimization |
| title |
Electrospun nanofibrous poly (Lactic Acid)/Titanium dioxide nanocomposite membranes for cutaneous scar minimization |
| spellingShingle |
Electrospun nanofibrous poly (Lactic Acid)/Titanium dioxide nanocomposite membranes for cutaneous scar minimization Marsi, Teresa C. O. PLA nanocomposites electrospinning cutaneous scarring gene expression in vivo Engenharia e Tecnologia::Engenharia dos Materiais Science & Technology |
| title_short |
Electrospun nanofibrous poly (Lactic Acid)/Titanium dioxide nanocomposite membranes for cutaneous scar minimization |
| title_full |
Electrospun nanofibrous poly (Lactic Acid)/Titanium dioxide nanocomposite membranes for cutaneous scar minimization |
| title_fullStr |
Electrospun nanofibrous poly (Lactic Acid)/Titanium dioxide nanocomposite membranes for cutaneous scar minimization |
| title_full_unstemmed |
Electrospun nanofibrous poly (Lactic Acid)/Titanium dioxide nanocomposite membranes for cutaneous scar minimization |
| title_sort |
Electrospun nanofibrous poly (Lactic Acid)/Titanium dioxide nanocomposite membranes for cutaneous scar minimization |
| author |
Marsi, Teresa C. O. |
| author_facet |
Marsi, Teresa C. O. Ricci, Ritchelli Toniato, Tatiane V. Vasconcellos, Luana M. R. Elias, Conceição de Maria Vaz Silva, Andre D. R. Furtado, Andre S. A. Magalhães, Leila S. S. M. Silva-Filho, Edson C. Marciano, Fernanda R. Zille, Andrea Webster, Thomas J. Lobo, Anderson O. |
| author_role |
author |
| author2 |
Ricci, Ritchelli Toniato, Tatiane V. Vasconcellos, Luana M. R. Elias, Conceição de Maria Vaz Silva, Andre D. R. Furtado, Andre S. A. Magalhães, Leila S. S. M. Silva-Filho, Edson C. Marciano, Fernanda R. Zille, Andrea Webster, Thomas J. Lobo, Anderson O. |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade do Minho |
| dc.contributor.author.fl_str_mv |
Marsi, Teresa C. O. Ricci, Ritchelli Toniato, Tatiane V. Vasconcellos, Luana M. R. Elias, Conceição de Maria Vaz Silva, Andre D. R. Furtado, Andre S. A. Magalhães, Leila S. S. M. Silva-Filho, Edson C. Marciano, Fernanda R. Zille, Andrea Webster, Thomas J. Lobo, Anderson O. |
| dc.subject.por.fl_str_mv |
PLA nanocomposites electrospinning cutaneous scarring gene expression in vivo Engenharia e Tecnologia::Engenharia dos Materiais Science & Technology |
| topic |
PLA nanocomposites electrospinning cutaneous scarring gene expression in vivo Engenharia e Tecnologia::Engenharia dos Materiais Science & Technology |
| description |
The animal study was reviewed and approved by 10/2015-CEUA/ICT/CJSC-UNESP. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019 2019-01-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/62736 |
| url |
http://hdl.handle.net/1822/62736 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Marsi TCO, Ricci R, Toniato TV,Vasconcellos LMR, Elias CdMV,Silva ADR, Furtado ASA,Magalhães LSSM, Silva-Filho EC,Marciano FR, Zille A, Webster TJ andLobo AO (2019) ElectrospunNanofibrous Poly (LacticAcid)/Titanium DioxideNanocomposite Membranes forCutaneous Scar Minimization.Front. Bioeng. Biotechnol. 7:421.doi: 10.3389/fbioe.2019.00421 2296-4185 10.3389/fbioe.2019.00421 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Frontiers Media |
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Frontiers Media |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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